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Skip Navigation LinksJRI > Archive > April-June 2003, Volume 4, Issue 2 > The effect of sperm chromatin anomalies on pronucleus different size after IVF and ICSI



Volume 4, Issue 2, Number 14 / April-June
(pages 137-145)


The effect of sperm chromatin anomalies on pronucleus different size after IVF and ICSI




 Corresponding Author
1- Embryology Department, Royan Institute, Iranian Academic Center for Education, Culture & Research (ACECR), Isfahan, Iran
2- Andrology Department, Royan Research Center, Isfahan, Iran

3- Embryology Laboratory, Isfahan Fertility & Infertility Center, Isfahan, Iran

4- Department of Embryology, Faculty of Medicine, Isfahan Medical Science University, Isfahan, Iran

Department of Embryology, Faculty of Medicine, Isfahan Medical Science University, Isfahan, Iran

Department of Embryology, Faculty of Medicine, Isfahan Medical Science University, Isfahan, Iran

Department of Embryology, Faculty of Medicine, Isfahan Medical Science University, Isfahan, Iran

Department of Embryology, Faculty of Medicine, Isfahan Medical Science University, Isfahan, Iran

Department of Embryology, Faculty of Medicine, Isfahan Medical Science University, Isfahan, Iran


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Abstract
Pronucleus size, morphology and orientation are known to affect embryo quality, implantation and pregnancy success. The present study was aimed at evaluation of the possible relationship between sperm chromatin maturity tests (CMA3, aniline blue, SDS, SDS+EDTA) and semen parameters with percentage of zygotes with pronucleus size asynchrony. Semen of 115 men from IVF and ICSI couples were analyzed for sperm concentration, motility, morphology and the said tests. At least 17-19 hours after sperm insemination or ICSI, oocytes were scored for presence and relative size of their pronuclei and then the relationship between percentage of zygotes with pronucleus size asynchrony and semen parameters and the above tests was evaluated. A positive significant relationship was obtained between percentage of zygotes with unequal pronucleus size with protamine deficiency, excessive histones and ability of sperm to undergo decongestion in ICSI cases. However, no correlation was observed between this phenomenon and any of semen parameters and aforementioned tests in IVF cases. The result of this study and other studies show that evaluation of chromatin status is of paramount importance, since protamine deficiency not only directly affects fertilization rate, but also affects pronuclear morphology which influences embryo development, implantation and pregnancy success in IVF and ICSI. The effect of abnormal chromatin status on development of zygote might be mediated via abnormal pronucleus formation since sperms with abnormal chromatin are injected into oocytes during ICSI.

Keywords: Sperm chromatin, Pronucleus size asynchrony, ICSI, IVF, Protamine deficiency


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References
  1. Palermo G.، Joris H.، Devrory P.، Van Steirteghem A.C. Pregnancy after intracytoplasmic injection of single spermatozoa into an oocyte. Lancet. 1992; 340:17-18.
  2. Van Steirteghem A.C., Liu J., Joris H., Nagy Z., Janssenwillen C., Tournay H., Deroey P.C. Higher success rate by intracytoplasmic sperm injection by sub zonal insemination: Report of a second series of 300 consecutive treatment. Hum Reprod. 1993;8: 1055-60.
  3. Iranpour F.G., Nasr-Esfahani M.H., Valojerdi M.R., al-Taraihi T.M. Chromomycin A3 staining as a useful tool for evaluation of male fertility. J Assist Reprod Genet. 2000;17(1):60-6.
  4. Sakkas D., Urner F., Bianchi P.G., BizzaroD., Wagnar I., Jaquenoud N., Manicardi G., Campana I. Sperm chromatin anomalies can influence decongestion after intracytoplasmic sperm injection. Hum Reprod.1996;11:837-43.
  5. Nasr-Esfahani M.H., Razavi S., Mardani M. Relation between different human sperm nuclear maturity tests and in vitro fertilization. J Assist Reprod Genet. 2001;18 (4):199-205.
  6. Esterhuizen A.D., Franken D.R., Lourens J.G.H., Van Zyl C., Muller I.I., Van Rooyen L.H. Chromatin packaging as an indicator of human sperm dysfunction. J Assist Reprod Genet. 2000;17(9):508-14.
  7. Ludwig M., Schopper B., Katalinic A., Sturm R., Al-Hasani S., Diedrich K. Experience with the elective transfer of two embryos under the conditions of the German embryo protection law: results of retrospective data analysis of 2573 transfer cycles. Hum Reprod. 2000;15(2):319-24.
  8. Scott L., Alvero R., Lenodires M., Miller B. The morphology of human pronuclear embryo is positively related to blastocyst development and implantation. Hum Reprod. 2000; 15(11): 2394-403.
  9. Wittemer C., Bettahar-Lebugle K., Ohl J., Rongieres C., Nisand I., Gerlinger P. Zygote evaluation: an efficient tool for embryo selection. Hum Reprod. 2000;15(12):2591-7.
  10. Garello C., Barker H., Rai J., Montgomery S., Wilson P., Kennedy C.R., Hartshorne G.M. Pronuclear orientation, polar body placement, and embryo quality after intracytoplasmic sperm injection and in-vitro fertilization: further evidence for polarity in human oocytes? Hum Reprod. 1999;14(10):2588-95.
  11. Goud P., Goud A., Van Oostveldt P., Van der Elst J., Dhont M. Fertilization abnormalities and pronucleus size asynchrony after intracytoplasmic sperm injection are related to oocyte post maturity. Fertil Steril.1999;72(2):245-52.
  12. Ma S., Yuen H. Intracytoplasmic sperm injection could minimize the incidence of prematurely condensed human sperm chromosomes. Fertil Steril. 2001;75(6):1095-101.
  13. Fishel S., Aslam I., Tesarik J. Spermatid conception: A stage too early, or a time too soon? Hum Reprod. 1996;11(3):1371-5.
  14. Terquem A., Dadoune J.P. Aniline blue staining of human spermatozoa chromatin: Evaluation of nuclear maturation.1983; In Andr J (editor), The sperm cell, J andr (ed), London, Martinus Nijhoff publishers, pp 696-701.
  15. Gonzales G.F., Salirrosas A., Dicina-Torres L.N., Sanchez A., Villena A. Use of clomiphene citrate in the treatment of men with high sperm chromatin stability. Fertil Steril. 1998;69:1109-114.
  16. Kruger T.F., Acosta A.A., Simmons K.F., Swanson R.J., Matta J.F., Oehninger S. Predictive value of abnormal sperm morphology in vitro fertilization. Fertil Steril. 1988;49:112-7.
  17. Perrault S.D., Barbee R.R., Slott V.L. Importance of glutathione in the activity and maintenance of sperm nuclear decondensing activity in maturing hamster oocytes. Dev Biol. 1988;125:181-6.
  18. Yoshida M., Ishigaki K., Nagai T., Chikyu M., Purset V.G. Glutathione concentration during maturation and after fertilization in pig oocytes: relevance of the ability of the oocytes to form male pronucleus. Biol Reprod. 1993;49: 89-94.
  19. Flaherty S.P., Payne D., Matthews C.D. Fertilization failures and abnormal fertilization after intracytoplasmic sperm injection. Hum Reprod. 1998;13(Suppl 1): 155-64.
  20. Bedford J.M., Bent M.J., Calvin H. Variation in the structural character and stability of the nuclear chromatin in morphology normal human spermatozoa. J Repod Fertil. 1973;33:19-29.
  21. Perrault S.D. Chromatin remodeling in mammalian zygotes. Mutat Res. 1992;296:43-55
  22. Sakkas D., Manicardi G.C., Tomlinson M., Mandrioli M., Bizzaro D., Bianchi P.G., Bianchi U. The use of two density gradient centrifugation techniques and swim up method to separate spermatozoa with chromatin and nuclear DNA anomalies. Hum Reprod. 2000; 15: 1112- 1116.
  23. Hammadeh M.E., Kuhnen A., Amer A.S., Rosenbaum P., Schmidth W. Comparison of sperm preparation methods: effect on chromatin and morphology recovery rates and their consequences on the clinical outcome after in vitro fertilization embryo transfer. Int J Androl. 2001;24(6):360-8.
  24. Foresta C., Zorzi M., Rossato M., Varotto I. Sperm nuclear instability and staining with aniline blue:Abnormal persistence of histones in spermatozoa in infertile men. Int J Androl. 1992;15:330-7.
  25. Sakkas D., Urner F., Bizzaro D., Manicardi G., Bianchi U., Shoukir Y., Campana A. Sperm nuclear DNA damage and altered chromatin structure :Effect on fertilization and embryo development. Hum Reprod.1998;13:11-19.
  26. Nasr-Esfahani M.H., Behdadi Pour Z., Abbasi H., Zaman Soltani F., Mardani M., Abuhamzeh B. The effect of varicocelectomy on sperm parameters, membrane integrity and chromatin condensation. Yakhteh (cell) 2000;5:7-11.
  27. Fulka J., Horska M., Moor R.M., Fulka J. and Kanaka J. Oocyte-Specific modulation of female pronuclear development in mice. Dev Biol. 1996;178:1-12.



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