- Mahmoud Dehghani Ashkezari
- - Department of Cellular and Molecular Biology, Molecular Research Center, Azad University, Yazd, Iran
- Seyyed Mehdi Kalantar
 - - Department of Reproduction & Genetics, Research & Clinical Center For Infertility, Yazd University of Medical Sciences & Health Services, Yazd, Iran
- Kazem Parivar
- - Department of Biology, Faculty of Sciences, Islamic Azad University, Science & Research Campus, Tehran, Iran
- Abbas Aflatoonian
- - Department of Obstet . and Gynecol Research and Clinical Center for Infertility, Faculty of Medicine, Yazd Shahid Sadooghi University of Medical Sciences, Yazd, Iran
Received: 1/1/2006 Accepted: 1/1/2006 - Publisher : Avicenna Research Institute |
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Abstract
Introduction: Assisted reproductive technologies have been used for the treatment of a consi-derable number of infertile couples. Conduction of several cycles of treatment, spending a lot of time, money and energy and the probable complications accompanying repeated anesthesia have made researchers find ways to predict the outcome of different methods used for the treatment of infertility. Male factor infertility is accountable for fifty percent of infertilities. Although semen analysis is an initial test to evaluate male fertility potentials but the results do not always predict fertilization outcomes. Sperm function tests have been suggested to predict the fertilization rate in ART treatment cycles. The objective of this study was to evaluate the diagnostic capabilities of double-stranded DNA in fertilization rate predictions. Materials & Methods: 100 infertile men were randomly selected. Based on WHO's 1999 cri-teria, semen analysis for each case was performed. DNA evaluation was performed by using Acridine orange. According to the fertilization rates (FR), the cases were divided into 3 groups: group I with FR>50%, group II with FR<50 % and group iii with a total fertilization failure (tff). the results were analyzed by using anova, correlation coefficient, and calculation of the area under receiver operating characteristic (roc) plot. the level of significance was consi-dered 5%. for the prediction of dna normality likelihood and the best cut-off points for the variables, calculation of the area under the roc plot was employed. results: there were no significant differences between fertilization rates (fr) and sperm para-meters in ivf treatment cycles. only a weak correlation was observed between tail defects and fr. regression analysis showed a correlation between double-stranded dna & fertilization rates (p="0.04)." the analysis of variance for the mean of double-stranded dna in cases with fr>50%, FR<50 % and tff showed a significant difference at the level of p<0.05. roc analysis showed that at 50% fertilization rate, the cut-off point for dna normality was 47.25%. conclusion: although semen analysis is an initial test for male fertility but it is not adequate for the prediction of ivf results. the use of dna normality function tests in conjunction with semen analysis may be helpful in choosing treatment protocols and predicting fertility success rates at 50%, < 50% and t.f.f. levels.< pan>
Keywords:
DNA normality, Double- stranded DNA, Acridine orange, IVF To cite this article:References
- Franken D.R., Acosta A.A., Kruger T.F., et al. The hemizona assay: its diagnostic role in male factor infertility. Fertil Steril.1993;59:1075-1080.
- Kalantar S.M., Lenton E.A., Cooke. Evaluating the ability of biological substancesfor induction of acro-some reaction in normospermic samples. Midd East Fertil Soci J.2000;5(2):71-78.
- World Health Organization. WHO Laboratory Manual for the Examination of Human Semen and Semen-Cer-vical Mucus Interaction. 4th Edition. Cambridge,United Kingdom: Cambridge University Press;1999.
- Hoshi K., Katayose H. The relationship acridine orange fluorescence of sperm nuclei and the fertilising ability of humun sperm. Fertil Steril.1996;66:634-639.
- Kalantar S.M. Dehghani Ashkezari M., Parivar K. Correlation between double stranded DNA and fertili-zation rate in infertile couples. 13th world congress on in vitro fertilization-Assisted Reproduction & Genetics. Istanbul, Turkey, May 26- 29, 2005.
- Lopes S., Sun J.G., Jurisicova A. et al. Sperm deoxy-ribonucleic acid fragmentation is increased in poor-quality semen samples and correlates with failed fertili-zation in intracytoplasmic sperm injection. Fertil Steril. 1998;69:528-532.
- Sakkas D., Urner F., Bianchi P.G., et al. Sperm chromatin anomalies can influence decondensation after intracytoplasmic sperm injection. Hum Reprod. 1996;11:837-843.
- Sun J.G., Jurisicova A., Casper R.F. Detection of deoxyribonucleic acid fragmentation in human sperm: correlation with fertilization in vitro. Biol Reprod. 1997;56:602-607.
- Spano M., Kolstad H., Larsen S.B., et al. The appli-cability of the flow cytometric sperm structure chroma-tin assay in epidemiological studies. Hum Reprod. 1998;13:2495-2505.
- Evenson D.P., Jost L.K. Utility of sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic. Hum Reprod.1999;14:1039-1049.
- Jeulin C., Feneux D., Serres C., et al. Sperm factors to failure of human in-vitro fertilization. J Reprod Fertil.1986;76:735-744.
- Tejada R.I., Mitchell J.C., Norman A., Marik J.J. and Friedman S. A test for the practical evaluation of male fertility by acridine orange (AO) fluorescence. Fertil Steril.1984;42:8791.
- Bartoov B., Etes F., Morphlogical characterization of abnormal human spermatozoa using transmassion elec-tron microscopy. Arch Androl.1980;5:305-322.
- Molina J., Castilla J.A., Gil T., Hortas M.L., Vergara F., Herruzo A. Influence of incubation on the chro-matin condensation and nuclear stability of human spermatozoa by flow cytometry. Hum Reprod.1995; 10:1280-6.
- Engh E., Clausen O.P. Relationship between sperm quality and chromatin condensation measured by DNA fluorescence using flow cytometry. Int J Androl.1992; l(15):407-415.
- Liu D.Y., Baker H.W.G. Tests of human sperm func-tion and fertilization in vitro. Fertil Steril.1992;58:465-483.
- Colleu D., Lescoat D., Boujard D., Le Lannou D. Human sperm nuclear marturity in normozoospermia and sthenozoospermia. Arch Androl.1988;21:155-62.
- Hofmann N., Hilscher B., Morchen B., et al. Compa-rative studies on various modes of classification of morphology of sperm heads and results in in vitro fertilization-a preliminary report. Andrologia.1995;27: 19-23.
- Chapman J.C., Michael S.D., et al. Proposed mecha-nism for sperm chromatin condensation/ deconden-sation in the male rat. Reprod Biol Endocrinol.2003; 1(1):20.
- Irvine D.S., Twigg J.P., Gordon E.L., Fulton N., Milne P.A., Aitken R.J. DNA integrity in human sper-matozoa: relationships with semen quality. J Androl. 2000;21:33-44.
- Larson K.L., DeJonge C.J., Barnes A.M., Jost L.K., Evenson D.P. Sperm chromatin structure assay parame-ters as predictors of failed pregnancy following assisted reproductive techniques. Hum Reprod.2000;15:1717-1722.
- Liu D.Y., Baker H.W.G. A new test for the assess-ment of sperm-zona pellucida penetration: relationship with results of other sperm tests and fertilization in vitro. Hum Reprod.1994;9:489-496.
- Duran E.H., Morshedi M., Taylor S., Oehninger S. Sperm DNA quality predicts intrauterine insemination outcome: a prospective cohort study. Hum Reprod. 2002;17(12):3122-3128.
- Erenpreiss J., Hlevicka S., Rauda R. High impact of male infertility in barren couples in Latvia, 1998-2001. Proc Latv Acad Sci Ser B.2002;56:48-51.
- Seli E., Gardner D.K., Schoolcraft W.B., Moffatt O., Sakkas D. Extent of nuclear DNA damage in ejaculated spermatozoa impacts on blastocyst development after in vitro fertilization. Fertil Steril. 2004;82:378-383.
- Hammadeh M.E., Zeginiadov T., Rosenbaum P., Georg T., Schmidt W., Strehler E. Predictive value of sperm chromatin condensation (aniline blue staining) in the assessment of male fertility. Arch Androl.2001;46: 99.
- Zweig M.H., Campbell G. Receiver operating charac-teristic (ROC) plots: A fundamental evaluation tool in clinical medicine. Clin Chem.1993;9:561-577.