JRI 

Maryam Nazm Bojnordi
- Anatomy Department, Faculty of Medical Sciences, Tarbiyat Modares University, Tehran, Iran
Mojdeh Salehnia Corresponding Author
- Anatomy Department, Faculty of Medical Sciences, Tarbiyat Modares University, Tehran, Iran
Abdolamir Allameh
- Department of Biochemistry, Medical Faculty, Tarbiat Modarres University, Tehran, Iran

Received: 4/1/2006 Accepted: 4/1/2006 - Publisher : Avicenna Research Institute

Related Articles

 

Other Format

 


Abstract

Introduction: Acid phosphatase (ACP) is a lysosomal enzyme which contributes in ovarian metabolic functions such as oocyte maturation, resumption of mitotic divisions, germinal vesicle breakdown and ovulation. It digestes the corpus lutetium and helps the atresia of follicles by autophagia and hetrophagia activities. Considering the hormonal control of this enzyme, the present study was designed to deterimine the ovarian ACP activity after ovulation stimulation by the administration fo PMSG and hCG during preimplantation period. Materials & Methods: For this purpose a number of 6 to 10-week old female NMRI mice were selected and randomly divided into control and hyperstimulated groups after the administeration of PMSG and hCG, and later to pregnant and pseudopregnant groups. The mice were rendered pseudopregnant by mechanical vaginal stimulatin. Five mice in each group were sacrificed by cervical dislocation at the first to the sixth day of pregnancy for biochemical assays. The ovarian samples were obtained and were hemo-genated and centrifuged at 14000 g. The activity of the enzyme was determined using paranitrophenyl phosphate as substrate and later the specific activity of the enzyme was calculalated according to the amount of total protein. The data were analysed by Mann Whitheny test. Statistical significance was indicated by a P value less than 0.05. For hisotchemical evaluations, the sampels were obtained from one of the ovaries in each mouse and then 5 m thick cryosections were prepared. Cryosections were stained by Goumory method. Results: The ACP activity of ovarian tissues in the first day of pregnancy in the normal pregnant and pseudopregnant control groups, hyperstimulated normal pregnant and pseudopregnant groups were 0.340.04 IU/mg, 0.390.04 IU/mg, 0.40.08 IU/mg, 0.450.01 IU/mg respectively and in the fourth day were 0.690.1 IU/mg, 0.61 0.06 IU/mg, 1.090.10 IU/mg and 0.79 0.05 IU/mg. The results showed that biochemical findings correlated with histochemical observations. The ACP reaction changes were seen mainly in granulosa cells with a minimum enzyme activity in the first day (zero activity) and a maximum activity in the fourth day of pregnancy (+3). Conclusion: The increased ACP activity on the 3rd- 4th days of pregnancy, may be due to the este-roidogenic activity of granulosa cells. Also, the results showed that ovarian hyperstimulation could not change the pattern of ovarian ACP activity during early stages of pregnancy. More research is required in this area for a better understanding of the processes.


Keywords: Acid phosphatase, Ovulation induction, Ovary, Implantation, Pseudopregnancy, Preimplantation, Esteroidogenesis


To cite this article:


References

  1. Brayman M., Thathiah A., Carson D. MUCI: A multi-functional cell surface component of reproductive tissue epithelial. Reprod Biol.2004;2: 4-8.
  2. Aplin J.D. The cell biological basis of human implan-tation. Obstet Gynecol.2000;14:757-764.
  3. Tsiligiann T.H., Karagiannidis A., Saratsis P., Brikas P. Enzyme activity in bovine cervical mucus during spon-taneous and induced estrus. J Vet Res.2003;67:189-93.
  4. Bull H., Murray P., Thomas D., Fraser A., Nelson P. Acid phosphatase. J Clin Pathol.2002;55:65-72.
  5. Flood P.F., Tyler N.J.C., Read E.K., Rodway M.J., Chedrese P.J. Ovarian and placental production of pro-gesterone and oestradiol during pregnancy in reindeer. Anim Reprod Sci.2005;85:147-162.
  6. Cheema R., Dhanju C.K., Matharoo J.S. Response rela-ted enzymatic changes in ovaries of superovulated mice. Exp Biol.2003;41(2):171-173.
  7. Imai K., Khandoker M., Yonai M., Takashashi T., Sato T., Ito A. Matrix metalloproteinases-2 and- 9 activities in bovine follicular fluid of differentsized follicles: relationship to intrafollicular inhi-bin and steroid con-centrations. Domest Anim Endocrinol.2003;24:171-83.
  8. Havelok J., Rainey W., Carr B. Ovarian granulosa cell lines. Mol Cell Endocrinol.2004;228:67-78.
  9. Wang I., Fraser I. Lysosomes: An important mediator in the female reproductive tract. Obstet Gynecol.1989; 45(1):18-33.
  10. Kleinman D., Insler V., Leiberman J., Glezerman M., Albotiano S., Potashnik G., et al. Acid phosphatase levels in follicular fluids following induction of ovula-tion in in vitro fertilization patients. J In Vitro Fert Embryo Transf.1987;4(3):181-4.
  11. Banos M.E., Rosales A.M., Ballesteros L.M., Hrnan-dez O., Rosado A. Changes in lysosomal enzyme acti-vities in pre-ovulatory follicles and endometrium of PMSG superovulated rats. Arch Med Res.1996;27(1): 49-55.
  12. Tavanioton A., Albano A., Smitz J., Devroey P. Im-pact of ovarian stimulation on corpus luteum function and embryonic implantation. J Reprod Immunol.2002; 55:123-30.
  13. Rosales T., Avalos R., Vergara O., Hernandez P., Ballesteros L., Garcia M. Multiparametric study of atresia in ewe antral follicle: histology, flow cytomet-ry, internucleosomal DNA fragmentation, and lysoso-mal enzyme activities in granulosa cells and follicular fluid. Mol Reprod Dev.2000;55 (3):270-81.
  14. Beckman G., Beckman L., Lofstrand T. Acid and alkaline phosphatase in amniotic fluid in normal and complicated pregnancy. Act Obstet Gynecol.1978;57: 1-5.
  15. Lindhard A., Ley U., Ravn V., Islin H., Hviid T., Rex .S, et al. Biochemical evaluation of endometrial func-tion at the time of implantation. Fertil Steril.2002;78 (2):221-233.
  16. Wang I., Fraster I., Barsamian S., Monconi F., Street D., Cornillie F., et al. Endometrial lysosomal enzyme activity in ovulatory dysfunctional uterine bleeding, IUCD users and post-partum women. Mol Hum Reprod.2000;6 (3):258-263.
  17. Krajni M., Lenhardt L., Valocky L., Cigankova V., Kostecky M., Maragek I. Activity of alkaline and acid phosphatase and non specific esterase in the endomet-rium and oviduct of post partum does. Elsevier, 2003.
  18. Rosales T., Avalos R., Vergara O., Hernandez P., Ballesteros L., Garcia M. Multiparametric study of atresia in ewe antral follicle: histology, flow cytometry, internucleosomal DNA fragmentation, and lysosomal enzyme activities in granulosa cells and follicular fluid. Mol Reprod Dev.2000;55(3): 270-81.
  19. Bucci M., Murphy C.R. Hormonal control of enzyme activity during the plasma membrane transformation of uterus epithlial cells. Cell Biol. 2001;25(9):859-871.
  20. Henderson K.A., Cupps P.T. Acid and alkaline phos-phatase in bovine antral follicles. Nat Sci.1989;13: 1363-1369.
  21. Ertzeid G., Storeng R. The impact of ovarian stimula-tion on implantation and fetal development in mice. Hum Reprod.2001;16(2):221-225.
  22. Dhanju C.K., Sangha G.K., Sekhon P.K. Biochemical status of ovaries after induction of superovulation on different days of estrus cycle in mice. Ind J Exp Biol. 2001;39(8):777-80.
  23. Lindhim S., Sauer M., Carmina E., Chang P., Zim-merman R., Rogerio A. Circulating levels during ovu-lation induction: relation to adiposity and ovarian mor-phology.Fertil Steril.2000;73(3):493-498.
  24. Emadi M., Salehnia M. The morphological expression of endometrial pinopodes during implantation in mice after ovarian stimulation and pro-gesterone injection. Yakhteh.2004;5(20):140-145.

COPE
SID
NLM
AJMB
IJBMLE
IJBMLE

Home | About Us | Current Issue | Past Issues | Submit a Manuscript | Instructions for Authors | Subscribe | Search | Contact Us

"Journal of Reproduction & Infertility" is owned, published, and managed by Avicenna Research Institute .
Creative Commons License

This work is licensed under a Creative Commons Attribution –NonCommercial 4.0 International License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.

Journal of Reproductoin and Infertility (JRI) is a member of COMMITTEE ON PUBLICATION ETHICS . Verify here .

©2024 - eISSN : 2251-676X, ISSN : 2228-5482, For any comments and questions please contact us.