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Skip Navigation LinksJRI > Archive > July-September 2006, Volume 7, Issue 2 > Intrafollicular fluid antigamete antibodies in infertile patient candidates for ICSI



Volume 7, Issue 2, Number 27 / July-September
(pages 101-110)


Intrafollicular fluid antigamete antibodies in infertile patient candidates for ICSI




 Corresponding Author
1- Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
2- Monoclonal Antibody Research Center, Avicenna Research Institute (ACECR), Tehran, Iran

Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

Monoclonal Antibody Research Center, Avicenna Research Institute (ACECR), Tehran, Iran

Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

Monoclonal Antibody Research Center, Avicenna Research Institute (ACECR), Tehran, Iran

Department of Cardiac Surgery and Cardiology, Rajaie Heart Center, Tehran, Iran


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Abstract
Introduction: Immunologic disturbances must be considered as a major cause of infertility. Antigamete antibodies like antisperm antibodies (ASA) and to anti-zona antibodies (AZA) seem to be implicated in the etiology of infertility. These antibodies affect fertilization and embryo development. It is important to screen these antibodies in infertile women who are candidates for in-vitro fertilization (IVF), because the presence of these antibodies may switch the treatment from IVF to intra-cytoplasmic microinjection (ICSI). The objective of this study was to deter-mine the presence of ASA and AZA in the follicular fluids (FF) of women who sought candida-cy for ICSI. Materials & Methods: In this prospective study, the follicular fluids of 96 infertile women (20 to 39 years old, mean 31.5±5.1), who were candidates for ICSI, were evaluated. According to the etiologies, 80 women had explained whereas 16 had unexplained infertility. All the follicular fluids were evaluated for the presence of ASA by ELISA and Sperm MAR test and also for the presence of AZA by ELISA. The data were analyzed by Chi-square test using SPSS soft-ware and the significance level was considered p<0.05. Results: According to the results of ELISA and Sperm MAR test, none of the patients had ASA in their follicular fluids. However, twenty samples (20.8%) were positive for AZA. In patients with unexplained infertility, autoantibodies to zona pellucida were significantly higher in the follicular fluid than the group with proven etiologies for infertility (p=0.001). Conclusion: The low incidence of ASA and the high incidence of AZA in the infertile women in this study, especially in women with unexplained infertility in Iran have to be considered seriously. Determination of AZA is highly recommended in the evaluation of infertile couples, especially those with unexplained infertility.

Keywords: Anti- zona antibody, Follicular fluid, Unexpained infertility, In Vitro fertilization, Microinjection, Autoantibody, Antigamete antibody, Antisperm antibody


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References
  1. Bohring C., Krause W. Characterization of spermato-zoa surface antigens by antisperm and it’s influence on acrosomal exocytosis. Am J Reprod Immunol. 2003;50 (5):411-19
  2. Moustafa M., Ozornek M.H., Krussel J.S., Cupisti S., Bodden-Heidrich R., Koldovsky U., et al. The effect of anti gamete antibodies on the success of assisted Rep-roduction. Clin Exp Obstet Gynecol. 1997;24(2):7-9
  3. Dunbar B.S., Avery S., Lee V., Prasad S., Schwahn D., Schwoebel E., et al. The mammalian Zona Pellucida: its biochemistry, immunochemistry, molecular biology and developmental expression. Reprod Fertil Dev. 1994;6(3):331-47
  4. Shabanowitz R.B. O’ Rand M.G. Characterization of the human zona pellucida from fertilized and unferti-lized eggs. J Reprod Fertil. 1988,82:151
  5. Shivers C.A., Dunbar B.S. Autoantibodies to the Zona pellucida: A possible cause for infertility in women. Science.1977; 197:1082-1086
  6. Dietl J., Freye J., Mettler L. Fertility inhibition using low-dose immunization with procine zona. pellucidae. Am J Reprod Immunol.1982;2(3):153-6
  7. Shibahara H., Shiraishi Y., Hirano Y., Suzuki T., Takamizawa S., Suzuki M. Divesity of the inhibitory effects on fertilization by antisperm antibodies bond to the smface of ejaculated human sperm. Hum Reprod. 2003;18(7):1469-73
  8. Kakagawa K., Kamada M., Maegawa M., Irahara M., Aono T. Sperm-immobilizing antibodies block capaci-tation in human spermatozoa. Arch Androl. 2001;47 (2):135-42
  9. Check J.H., Bollendorf A., Katsoff D., Kozak J. The frequency of antisperm antibodies in the cervical mucus of women with poor postcoital tests and their effect on pregnancy rates. Am J Reprod Immunol. 1994;32(1):38-42
  10. Naz R.K. Modalities for treatment of antisperm anti-body mediated infertility: Novel Prospective. Am J Reprod Immunol. 2004;51(2):390-7
  11. Saragueta P., Lanuza G., Miranda P.V., Tzon J.G., Baranao J.L. Immunoglobulins from human follicular fluid induce the acrosome reaction in human sperm. Mol Reprod Dev. 1994;39(3):280-8
  12. Mardesic T., Ulcova-Gallova Z., Huttelova R., Muller P., Voboril J., Mikova M., et al. The influence of different types of antibodies on in vitro fertilization results. Am J Reprod Immunol. 2000;43(1):1-5
  13. طالبيان اصغر، زرناني اميرحسن، جدي‌تهراني محمود، آخوندي محمدمهدي، صادقي محمدرضا. طراحي روش الايزا با استفاده از آنتي‌ژن‌هاي سطح اسپرم جهت اندازه‌گيري آنتي‌بادي عليه اسپرم در مقايسه آن با تست آگلوتيناسيون Sperm Mar. باروري و ناباروري: سال پنجم(1382) شمارة 1. صفحات:34-23
  14. Putowski et al. The immunological profile of infertile women after repeated IVF failure. Eur J Obstet Gynecol Reprod Biol. 2004;112(2):192-6
  15. Unlu C., Bohmer S., Maas D.H. Immunological factors in extracorporeal fertilization. The role of antibodies against spermatozoa and zona pellucida antigen. Fortschr Med. 1990;108(23):450-3
  16. Kohl B., Kohl H., Krause W., Deichert U. The clini-cal significance of antisperm antibodies in infertile couples. Hum Reprod. 1992;7(10):1384-7
  17. Curtis P., Burford G., Amso N., Keith E., Shaw R.W. Assessment of the relevance of zona pellucida anti-bodies in serum and cervical mucus in patients who have fertilization failure during in vitro fertilization. Fertil Steril. 1991;56(6):1124-7
  18. Ivanova M., Djarkova T., Mollova M., Petrov M., Tikhomirova T., Dakhno F. Zona pellucida autoanti-bodies in women undergoing ART. Folia Biol(Praha). 1999;45(2):59-62
  19. Taneichi A., Shibahara H., Takahashi K., Sasaki S., Kikuchi K., Sato I., et al. Effects of sera from infertile women with sperm immobilizing antibodies on ferti-lization and embryo development in vitro in mice. Am J Reprod Immunol. 2003;50(2):146-51
  20. Kay D.J., Boettcher B. Comparison of the Sperm Mar test with the currently accepted procedures for detec-ting human sperm antibodies. Reprod Fertil De. 1992; 175-81
  21. Chiu W.W., Chamley L.W. Human seminal plasma prolactin-inducible protein is an immunoglobulin G-binding protein. J Reprod Immunol. 2003;60(2):97-111
  22. Naz R.K., Involvement of fertilization anti sperm (FA-1) in involuntary immuno infertility in humans. J Clin Invert. 1987;80(5):1375-83
  23. Menge A.C., Medley N.E., Mangione C.M., Dietrich J.W. The incidence and influence of antisperm anti-
  24. de Almedia M., Gazagne I., Jeulin C., Herry M., Belaisch-Allart J., Frydman R., et al. In vitro process-ing of sperm with autoantibodies and in- vitro fertiliza-tion results. Hum Reprod. 1989;4(1):49-53
  25. Mandelbaum S.L., Diamond M.P., DeCherney A.H. The impact of antisperm antibodies on human infertil-ity. J Urol.1987;138(1):1-8
  26. كاظميني سيد محمد، صديقي گيلاني محمدعلي، دادخواه فريد، هادي ندوشن حسين. تعيين آنتي‌بادي عليه اسپرم در سرم و مايع انزالي مردان آزواسپرم به منظور پيشگويي موفقيت استخراج اسپرم از بيضه. باروري و ناباروري: سال چهارم (1382) شمارة 4. صفحات:279-273
  27. Mhaskar A., Buckshee K., Talwar G.P.Autoantibodies to zona pellucida in tubectomized women. Contracep-tion. 1984;29(1):75-82
  28. Nishimoto T., Mori T., Yamada I., Nishimura T. Autoantibodies to zonapellucida in infertile and aged women. Fertil Steril. 1980;34(6):552-6
  29. Ulcova-Gallova Z., Babcova K., Novakova P., Mica-nova Z., Rokyta Z. Antizonal antibodies in ovulatory cervical mucus and in serum of patients with fertility disorders. Ceska Gynekol. 2004;69(3):215-8
  30. Papale M.L., Grillo A., Leonardi E., Giuffrida G., Palumbo M., Palumbo G. Assessment of the relevance of zona pellucida antibodies in follicular fluid of in-vitro fertilization (IVF) patients. Hum Reprod. 1994;9 (10):1827-31
  31. Chang T.H., Jin M.H., Wu T.C. Relationship of sperm antibodies in women and men to human in vitro ferti-lization, cleavage and pregnancy rate. Am J Reprod Immunol. 1993;30(2-3):108-12



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