ARI 
 JRI 
 ISERB 
blogger delicious digg diigo facebook googleplus linkedin netlog reddit twitter
Skip Navigation LinksJRI > Archive > April-June 2009, Volume 10, Issue 1 > Prevalence of Chlamydia trachomatis Infection in Fertile and Infertile Women; A Molecular and Serological Study



Volume 10, Issue 1, Number 38 / April-June
(pages 32-41)


Prevalence of Chlamydia trachomatis Infection in Fertile and Infertile Women; A Molecular and Serological Study




Vali-e- Asar Reproductive Health Research Center,Tehran Medical Sciences University, Tehran, Iran

 Corresponding Author
Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

Vali-e- Asar Reproductive Health Research Center,Tehran Medical Sciences University, Tehran, Iran

Vali-e- Asar Reproductive Health Research Center,Tehran Medical Sciences University, Tehran, Iran

Maternal-Fetal-Neonatal Health Research Center, Tehran University of Medical Sciences, Tehran, Iran

Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

1- Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
2- Department of Biostatistics and Epidemiology, International Branch of Tehran University of Medical Sciences, Tehran, Iran

Received: 11/15/2009 Accepted: 3/8/2009

Related Articles
in Google Scholar in PubMed

 

Other Format
pdfPDF Full Text (En) pdfPDF Full Text (Fa) pdfePUB Full Text (En) pdfPDF Abstract (En) pdfPDF Abstract (Fa) pdf BibTeX pdfRefMan pdfEndNote xmlPMC XML online readerPMC Reader

 


Abstract
Introduction: Chlamydia trachomatis is one of the most common sexually transmitted infections (STIs) in the world. About 80% of the infected women are asymptomatic, while ascending infections such as pelvic inflammatory disease (PID) and the resultant infertility due to fallopian tube occlusion are common manifestations. Due to the higher prevalence of C. trachomatis infection in infertile than fertile women and the importance of screening for this infection in different types of infertility, this study was undertaken to compare two serologic and ELISA methods for the diagnosis of the bacteria in the two groups.Materials and Methods: In this cross-sectional study, the participants included 233 infertile women attending Vali-e-Asr Infertility Clinic and 225 fertile women attending the Prenatal Clinic and Emergency Labor of Imam Khomeini Hospital. Each participant completed a researcher-devised questionnaire and subsequently 2 mls of peripheral blood for serological studies and 15 ml of the first catch urine (FCU) for molecular detection of the germ through Polymerase Chain Reaction (PCR) method were obtained.Results: PCR results showed C. trachomatis infection in 29 (13.8%) infertile and 19 (11.1%) pregnant participants with no significant statistical differences. Serological results showed presence of Chlamydia IgG in 20 (8.6%) infertile and 11 (4.9%) fertile participants and Chlamydia IgM was observed in 2 (0.9%) infertile and 4 (1.8%) fertile participants with no significant differences.Conclusion: The prevalence of C. trachomatis infection among fertile and infertile women by serological and molecular methods was not significantly different. However, considering the high specificity and sensitivity of PCR, it could be used as a noninvasive screening technique for C. trachomatis, compared to the invasive method of blood sampling.

Keywords: Chlamydia trachomatis, Infertility, Polymerase chain reaction, Pregnancy, Serology, Sexually transmitted infection, Tubal occlusion


To cite this article:



Figures, Charts, Tables

References
  1. Stamm WE, Jones RB, Batteiger BE, editors. Chla-mydia trachomatis (Trachoma, perinatal infections, lymphogranuloma venereum, and other genital infec-tions). Philadelphia: Elsevier Churchill Livingstone; 2005: 2239 p. (Mandell GL, Dolin R, Bennette JE, editors. Principles and practice of infectious diseases; vol. 2).
  2. Sciarra JJ. Sexually transmitted diseases: global import-ance. Int J Gynaecol Obstet. 1997;58(1):107-19.   [PubMed]
  3. Workowski KA, Levine WC, Wasserheit JN; Centers for Disease Control and Prevention, Atlanta, Georgia. US Centers for Disease Control and Prevention guide-lines for the treatment of sexually transmitted diseases: an opportunity to unify clinical and public health prac-tice. Ann Intern Med. 2002;137(4):255-62.   [PubMed]
  4. McGregor JA, French JI. Chlamydia trachomatis infec-tion during pregnancy. Am J Obstet Gynecol. 1991;164 (6 Pt 2): 1782-9. Review.   [PubMed]
  5. Sambrook J, Russell DW, editors. Molecular cloning: A laboratory manual. 3rd ed. Vol. 6, Preparation and analysis of eukaryotic genomic DNA. New York: Cold Spring Harbor Laboratory Press; 2001. 237 p.
  6. Chaudhry U, Saluja D. Detection of Neisseria gonor-rhoeae by PCR using orf1 gene as target. Sex Transm Infect. 2002;78(1):72.   [PubMed]
  7. Marushko IuV, Desiatnyk DH, Bychkova NH, Marush-ko TV, Iehipko HV. [Characteristics of modern diagno-sis of diseases caused by Chlamydia bacteria]. Lik Sprava. 2002;(7):3-6. Ukrainian.   [PubMed]
  8. Hacker NF, Moore JG. Essentials of obstetrics and gy-necology. 3rd ed. Philadelphia: WB Saunders Com-pany; 1998: p. 507-37.
  9. Sharma M, Sethi S, Daftari S, Malhotra S. Evidence of chlamydial infection in infertile women with fallopian tube obstruction. Indian J Pathol Microbiol. 2003;46 (4):680-3.   [PubMed]
  10. Practice Committee of American Society for Repro-ductive Medicine. Definitions of infertility and recur-rent pregnancy loss. Fertil Steril. 2008;90(5 Suppl): S60.   [PubMed]
  11. Debattista J, Gazzard CM, Wood RN, Allan JA, Allan JM, Scarman A, et al. Interaction of microbiology and pathology in women undergoing investigations for infertility. Infect Dis Obstet Gynecol. 2004;12(3-4): 135-45.   [PubMed]
  12. Siemer J, Theile O, Larbi Y, Fasching PA, Danso KA, Kreienberg R, et al. Chlamydia trachomatis infection as a risk factor for infertility among women in Ghana, West Africa. Am J Trop Med Hyg. 2008;78(2):323-7.   [PubMed]
  13. Rampersad J, Wang X, Gayadeen H, Ramsewak S, Ammons D. In-house polymerase chain reaction for affordable and sustainable Chlamydia trachomatis detection in Trinidad and Tobago. Rev Panam Salud Publica. 2007;22(5):317-22.   [PubMed]
  14. Badami N, Salari MH. Rate of Chlamydia tracho-matis, Mycoplasma hominis and Ureaplasma urea-lyticum in infertile females and control group. Iran J Public Health. 2001;30(1-2):57-60.   [Abstract]
  15. Chamani-Tabriz L, Tehrani M J, Zeraati H, Asgari S, Moini M, Rabbani H, et al. [A Molecular survey on prevalence of Chlamydia trachomatis infection in pregnant woman attending OB&GY clinics of Tehran]. Iran J Infect Dis Trop Med. 2007;13(41):45-50.Persian.
  16. Chen XS, Yin YP, Chen LP, Thuy NT, Zhang GY, Shi MQ, et al. Sexually transmitted infections among pregnant women attending an antenatal clinic in Fuzhou, China. Sex Transm Dis. 2006;33(5):296-301.   [PubMed]
  17. Tukur J, Shittu SO, Abdul AM. A case control study of active genital Chlamydia trachomatis infection among patients with tubal infertility in northern Nigeria. Trop Doct. 2006;36(1):14-6.   [PubMed]
  18. S?nmez S, S?nmez E, Yasar L, Aydin F, Coskun A, Süt N. Can screening Chlamydia trachomatis by sero-logical tests predict tubal damage in infertile patients? New Microbiol. 2008;31(1):75-9.   [PubMed]
  19. Omo-Aghoja LO, Okonofua FE, Onemu SO, Larsen U, Bergstrom S. Association of Chlamydia trachomatis serology with tubal infertility in Nigerian women. J Obstet Gynaecol Res. 2007;33(5):688-95.   [PubMed]
  20. Idahl A, Boman J, Kumlin U, Olofsson JI. Demonstra-tion of Chlamydia trachomatis IgG antibodies in the male partner of the infertile couple is correlated with a reduced likelihood of achieving pregnancy. Hum Reprod. 2004;19(5):1121-6.   [PubMed]
  21. Land JA, den Hartog JE. Chlamydia antibody testing in subfertile women. Drugs Today (Barc). 2006;42 Suppl A:35-42. Review.   [PubMed]
  22. Thomas K, Coughlin L, Mannion PT, Haddad NG. The value of Chlamydia trachomatis antibody testing as part of routine infertility investigations. Hum Reprod. 2000;15(5):1079-82.   [PubMed]
  23. Chamani-Tabriz L, Tehrani MJ, Akhondi MM, Mosavi-Jarrahi A, Zeraati H, Ghasemi J, et al. Chla-mydia trachomatis prevalence in Iranian women attending obstetrics and gynaecology clinics. Pak J Biol Sci. 2007;10(24):4490-4.   [PubMed]
  24. Chamani-Tabriz L, Tehrani MJ, Zeraati H, Asgari S, Tarahomi M, Moini M, et al. [A molecular survey of Chlamydia trachomatis infection in married women: a cross sectional study on 991 women]. Tehran Univ Med J. 2008;66(7):485-91. Persian.   [Abstract]
  25. Malik A, Jain S, Rizvi M, Shukla I, Hakim S. Chla-mydia trachomatis infection in women with secondary infertility. Fertil Steril. 2009;91(1):91-5.   [PubMed]
  26. Hvid M, Baczynska A, Deleuran B, Fedder J, Knud-sen HJ, Christiansen G, et al. Interleukin-1 is the initi-ator of fallopian tube destruction during Chlamydia trachomatis infection. Cell Microbiol. 2007;9(12): 2795-803.   [PubMed]
  27. Cravioto Mdel C, Matamoros O, Villalobos-Zapata Y, Pe?a O, Garc?a-Lara E, Mart?nez M, et al. [Prevalence of anti-Chlamydia trachomatis and anti-Neisseria gonorrhoeae antibodies in Mexican populations]. Salud Publica Mex. 2003;45(5 Suppl):S681-9. Review. Spanish.   [PubMed]
  28. Den Hartog JE, Land JA, Stassen FR, Slobbe-van Drunen ME, Kessels AG, Bruggeman CA. The role of chlamydia genus-specific and species-specific IgG antibody testing in predicting tubal disease in subfertile women. Hum Reprod. 2004;19(6):1380-4.   [PubMed]
  29. Wallace LA, Scoular A, Hart G, Reid M, Wilson P, Goldberg DJ. What is the excess risk of infertility in women after genital chlamydia infection? A systematic review of the evidence. Sex Transm Infect. 2008;84(3): 171-5.   [PubMed]
  30. Kanayama A, Fujihara E, Saika T, Kobayashi I, Onoye Y. [Detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine samples of males and females by the strand displacement amplification (SDA) method]. Kansenshogaku Zasshi. 2008;82(3): 182-6. Japanese.   [PubMed]
  31. Fedorova VA, Bannikova VA, Alikberov ShA, Eli-seev IuIu, Grashkin VA. [Comparative efficiency of detection of the causative agent of urogenital chla-mydiasis by immunofluorescence, polymerase chain reaction, and dot immunoassay]. Klin Lab Diagn. 2007;7:30-5. Russian.   [PubMed]



Home | About Us | Current Issue | Past Issues | Submit a Manuscript | Instructions for Authors | Subscribe | Search | Contact Us

"Journal of Reproduction & Infertility" is owned, published, and copyrighted by Avicenna Research Institute .
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.

Journal of Reproductoin and Infertility (JRI) is a member of COMMITTEE ON PUBLICATION ETHICS . Verify here .

©2016 - eISSN : 2251-676X, ISSN : 2228-5482, For any comments and questions please contact us.