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Skip Navigation LinksJRI > Archive > July-September 2010, Volume 11, Issue 2 > Evaluation of Serum Nitrite, Nitrate and Malondialdehyde Concentrations in Preeclampsia



Volume 11, Issue 2, Number 43 / July-September
(pages 105-112)


Evaluation of Serum Nitrite, Nitrate and Malondialdehyde Concentrations in Preeclampsia




 Corresponding Author
Department of Clinical Biochemistry, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Received: 8/23/2009 Accepted: 12/19/2009

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Abstract
Introduction: Preeclampsia is a complication of pregnancy that is associated with high blood pressure with an unknown etiology. There is much evidence that altered endothelial cell functions may play an important role in the pathogenesis of preeclampsia. The aims of this study were to determine serum levels of nitrite, nitrate and malondialdehyde (MDA) in pregnant women with and without preeclampsia. Materials and Methods: This observational-analytical study was performed in the third trimester of pregnancy on 35 pregnant primiparas with preeclampsia and 35 women with normal blood pressure. The women were recruited for the study among the mothers attending Andimeshk Urban Health Centers during March 2008 to March 2009. Aside from the demographic data, the gestational age and blood pres-sure were recorded too at the time of blood sampling. Serum levels of nitrite and nitrate were measured by Griess and MDA by thiobarbituric acid reactions. The data were statistically analyzed and compared between the two groups. Results: Serum levels of nitrite, nitrate and MDA, respectively were 12.6 ± 3.8, 23.8 ± 8.6 and 9.7 ± 2.8 mol/l in women with preeclampsia and 8.7 ± 2.5 (p < 0.001), 16.4 ± 5.4 (p < 0.01) and 6.34 ± 1.6 mol/l (p < 0.001) in the controls. The results depicted significantly higher serum concentrations of the three analyses in pregnant women with preeclampsia relative to the controls. Conclusion: Significantly higher serum concentrations of nitrite, nitrate and MDA in pregnant women with preeclampsia are suggestive of the role of these substances in the pathogenesis of the disease. Use of the three parameters may be useful in the early screening of preeclampsia.

Keywords: Blood pressure, Preeclampsia, Nitrate, Nitrite, Malondialdehyde, Third trimester, Pregnancy, Prenatal care


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References
  1. Lana K, Wagner MD. Diagnosis and Management of Preeclampsia. Am Fam Physician. 2004;70(12): 2317-24.   [Abstract]
  2. Rukmini MS, Kowsalya R, Pai B, Das P, Perriera J, Nandini M, et al. Plasma adenosine deaminase activity and antioxidant status in preeclampsia compared to healthy pregnant and nonpregnant women. Biomed Res. 2009;20(1):15-20.   [Abstract]
  3. Rao GM, Sumita P, Roshni M, Ashtagimatt MN. Plasma antioxidant vitamins and lipid peroxidation products in pregnancy induced hypertension. Indian J Clin Biochem. 2005;20(1):198-200.   [PubMed]
  4. Chang YJ, Lee WK, Kim HG. Oxidative stress in rat model of preeclampsia and clinical correlates. Korean J Physiol Pharmacol. 2007;11(3):129-133.   [Abstract]
  5. Ozan H, Ilcol Y, Kimya Y, Cengiz C, Ediz B. Plasma anti-oxidant status and lipid profile in nongravida women with a history of pre-eclampsia. J Obstet Gynaecol Res. 2002;28(5):274-9.   [PubMed]
  6. Teran E, Chedraui P, Vivero S, Villena F, Duchicela F, Nacevilla L. Plasma and placental nitric oxide levels in women with and without pre-eclampsia living at different altitudes. Int J Gynaecol Obstet. 2009;104(2):140-2.   [PubMed]
  7. Vatish M, Randeva HS, Grammatopoulos DK. Hormonal regulation of placental nitric oxide and pathogenesis of pre-eclampsia. Trends Mol Med. 2006;12 (5):223-33.   [PubMed]
  8. Sladek SM, Magness RR, Conrad KP. Nitric oxide and pregnancy. Am J Physiol. 1997;272(2 Pt 2):R 441-63.   [PubMed]
  9. Adiga U, D'souza V, Kamath A, Mangalore N. Antioxidant activity and lipid peroxidation in preeclampsia. J Chin Med Assoc. 2007;70(10):435-8.   [PubMed]
  10. Krishna Mohan S, Venkataramana G. Status of lipid peroxidation, glutathione, ascorbic acid, vitamin E and antioxidant enzymes in patients with pregnancy--induced hypertension. Indian J Physiol Pharmacol. 2007;51(3):284-8.   [PubMed]
  11. Paşaoğlu H, Bulduk G, Oğüş E, Paşaoğlu A, Onalan G. Nitric oxide, lipid peroxides, and uric acid levels in pre-eclampsia and eclampsia. Tohoku J Exp Med. 2004;202(2):87-92.   [PubMed]
  12. Seligman SP, Buyon JP, Clancy RM, Young BK, Abramson SB. The role of nitric oxide in the pathogenesis of preeclampsia. Am J Obstet Gynecol. 1994;171(4):944-8.   [PubMed]
  13. Begum S, Yamasaki M, Mochizuki M. The role of nitric oxide metabolites during pregnancy. Kobe J Med Sci. 1996;42(2):131-41.   [PubMed]
  14. Ranta V, Viinikka L, Halmesmäki E, Ylikorkala O. Nitric oxide production with preeclampsia. Obstet Gynecol. 1999;93(3):442-5.   [PubMed]
  15. Pasaoglu H, Bukan N, Bulduk G, Celen S. Lipid peroxidation, nitrate and nitrite levels in eclamptic and intrauterine growth retarded pregnancies. Turk J Med Sci. 2003;33(2):89-93.   [Abstract]
  16. Aksoy H, Aksoy Y, Ozbey I, Altuntas I, Akçay F. The relationship between varicocele and semen nitric oxide concentrations. Urol Res. 2000;28(6): 357-9.   [PubMed]
  17. Guevara I, Iwanejko J, Dembinska-Kiec A, Pankiewicz J, Wanat A, Anna P, et al. Determination of nitrite/nitrate in human biological material by the simple Griess reaction. Clin Chim Acta. 1998; 274(2):177-88.   [PubMed]
  18. Somogyi M. A method for the preparation of blood filtrates for the determination of sugar. J Biol Chem. 1930;86(2):643-53.   [Abstract]
  19. Rao B, Soufir JC, Martin M, David G. Lipid peroxidation in human spermatozoa as related to midpiece abnormalities and motility. Gamete Res. 1989;24(2):127-34.   [PubMed]
  20. Nourooz-Zadeh J, Tajaddini-Sarmadi J, McCarthy S, Betteridge DJ, Wolff SP. Elevated levels of authentic plasma hydroperoxides in NIDDM. Diabetes. 1995;44(9):1054-8.   [PubMed]
  21. Lorentzen B, Henriksen T. Plasma lipids and vascular dysfunction in preeclampsia. Semin Reprod Endocrinol. 1998;16(1):33-9.   [PubMed]
  22. Scholl TO, Leskiw M, Chen X, Sims M, Stein TP. Oxidative stress, diet, and the etiology of preeclampsia. Am J Clin Nutr. 2005;81(6):1390-6.   [PubMed]
  23. Tug N, Celik H, Cikim G, Ozcelik O, Ayar A. The correlation between plasma homocysteine and malondialdehyde levels in preeclampsia. Neuro Endocrinol Lett. 2003;24(6):445-8.   [PubMed]
  24. Takacs P, Kauma SW, Sholley MM, Walsh SW, Dinsmoor MJ, Green K. Increased circulating lipid peroxides in severe preeclampsia activate NF-kappaB and upregulate ICAM-1 in vascular endothelial cells. FASEB J. 2001;15(2):279-81.   [PubMed]
  25. Maseki M, Nishigaki I, Hagihara M, Tomoda Y, Yagi K. Lipid peroxide levels and lipids content of serum lipoprotein fractions of pregnant subjects with or without pre-eclampsia. Clin Chim Acta. 1981;115(2):155-61.   [PubMed]
  26. Walsh SW. Maternal-placental interactions of oxidative stress and antioxidants in preeclampsia. Semin Reprod Endocrinol. 1998;16(1):93-104.   [PubMed]
  27. Sharma JB, Sharma A, Bahadur A, Vimala N, Satyam A, Mittal S. Oxidative stress markers and antioxidant levels in normal pregnancy and preeclampsia. Int J Gynaecol Obstet. 2006;94(1):23-7.   [PubMed]
  28. Regan CL, Levine RJ, Baird DD, Ewell MG, Martz KL, Sibai BM, et al. No evidence for lipid peroxidation in severe preeclampsia. Am J Obstet Gynecol. 2001;185(3):572-8.   [PubMed]
  29. Paternoster DM, Stella A, Mussap M, Plebani M, Gambaro G, Grella PV. Predictive markers of preeclampsia in hypertensive disorders of pregnancy. Int J Gynaecol Obstet. 1999;66(3):237-43.   [PubMed]
  30. Mutlu-Turkoglu U, Aykac-Toker G, Ibrahimoglu L, Ademoglu E, Uysal M. Plasma nitric oxide metabolites and lipid peroxide levels in preeclamptic pregnant women before and after delivery. Gynecol Obstet Invest. 1999;48(4):247-50.   [PubMed]
  31. Norris LA, Higgins JR, Darling MR, Walshe JJ, Bonnar J. Nitric oxide in the uteroplacental, fetoplacental, and peripheral circulations in preeclampsia. Obstet Gynecol. 1999;93(6):958-63.   [PubMed]
  32. Nishikawa S, Miyamoto A, Yamamoto H, Ohshika H, Kudo R. The relationship between serum nitrate and endothelin-1 concentrations in preeclampsia. Life Sci. 2000;67(12):1447-54.   [PubMed]
  33. Gilbert JS, Ryan MJ, LaMarca BB, Sedeek M, Murphy SR, Granger JP. Pathophysiology of hypertension during preeclampsia: linking placental ischemia with endothelial dysfunction. Am J Physiol Heart Circ Physiol. 2008;294(2):H541-50.   [PubMed]
  34. Teran E, Escudero C, Moya W. Abnormal release of nitric oxide from nitrosoprotein in preeclampsia. Int J Gynaecol Obstet. 2006;92(3):260-1.   [PubMed]
  35. Teran E, Escudero C, Vivero S, Enriquez A, Calle A. Intraplatelet cyclic guanosine-3',5'-monophosphate levels during pregnancy and preeclampsia. Hypertens Pregnancy. 2004;23(3):303-8.   [PubMed]



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