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Skip Navigation LinksJRI > Archive > December 2010, Volume 11, Issue 4 > Effects of Vitamin E and Ginseng Extract on Fertility Changes Induced by Cyclophosphamide in Rats



Volume 11, Issue 4, Number 45 / December
(pages 227-237)


Effects of Vitamin E and Ginseng Extract on Fertility Changes Induced by Cyclophosphamide in Rats




Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran

Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran

 Corresponding Author
Department of Physiology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran

Department of Embryology, Faculty of Veterinary, Urmia University, Urmia, Iran

Received: 4/24/2010 Accepted: 6/28/2010

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Abstract
Background: Cyclophosphamide (CP) is a mustard alkylating agent used in the treatment of some neoplastic diseases such as leukemia, breast and pulmonary cancers. CP administration induces oxidative stress and has cytotoxic effects on normal cells, especially in the reproductive organs. A major side-effect of CP is the alteration of male reproductive function which may result in oligospermia or azoospermia. The aim of the present study was to evaluate the protective effects of vitamin E and ginseng extract on the reproductive system of male rats during cyclophosphamide administration. Methods: Fifty-six adult male Wistar rats (220±30 g) were randomly divided into seven groups of eight. To eliminate the stress induced by gavage, the animals in the first group were considered as the control group and only received water and food. The second group received the placebo for CP via gavage. The third group received CP, 6.1 mg/kg/day, through intraperitoneal administration. The fourth and fifth groups, respectively, received Ginseng, 500 mg/kg/day, and vitamin E 100 mg/kg/day via gavage. The eighth group received both antioxidants by intubation 1 h prior to CP administration for 50 days. The animals were sacrificed one day after the last injection. The testes, body weight, sperm parameters and fertility status of animals were evaluated at the end of the experiments. For the evaluation of fertility index, the male rats were mated with untreated female rats on the 40th day of the treatment period. Results: Cyclophosphamide decreased sperm count, lowered fertility rate and decreased testis weight while it increased the number of dead and abnormal sperms (p < 0.01). In addition, the number of pregnant animals and viable offspring were reduced too; while antioxidant use diminished the adverse effects of CP. Conclusion: The results of the study showed that antioxidative agents vitamin E and Ginseng could diminish the adverse effects of cyclophosphamide in the reproductive system of male rats during cyclophosphamide administration.

Keywords: Chemotherapy, Cyclophosphamide, Ginseng, Male infertility, Rat, Reproductive health, Sperm, Vitamin E


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