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Skip Navigation LinksJRI > Archive > January-March 2002, Volume 3, Issue 1 > Morphometric study of GnRH analogue, HMG and HCG effects on ultrastructure of human endometrial glandular epithelium in early luteal phase



Volume 3, Issue 1, Number 9 / January-March
(pages 4-12)


Morphometric study of GnRH analogue, HMG and HCG effects on ultrastructure of human endometrial glandular epithelium in early luteal phase




Histology Department, Faculty of Medicine, Tabriz Medical Sciences University, Tabriz, Iran

 Corresponding Author
1- Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
2- Majidi Infertility Center, Faculty of Medicine, Tabriz Medical Sciences University, Tehran, Iran

Anatomy Department, Faculty of Medicine, Tabriz Medical Sciences University, Tabriz, Iran

Anatomy Department, Faculty of Medicine, Shahid Beheshti Medical Sciences University, Tehran, Iran

Anatomy Department, Faculty of Medicine, Zahedan Medical Sciences University, Zahedan, Iran

Majidi Infertility Center, Faculty of Medicine, Tabriz Medical Sciences University, Tabriz, Iran

Majidi Infertility Center, Faculty of Medicine, Tabriz Medical Sciences University, Tabriz, Iran

Majidi Infertility Center, Faculty of Medicine, Tabriz Medical Sciences University, Tabriz, Iran


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Abstract
GnRH analogue, HMG and HCG administration are common protocol for ovulation induction in assisted reproductive technology (ART). Since implantation rate in stimulated ART cycles is lower than unstimulated cycles and as endometrium plays an important role in embryo receptivity, effect of this protocol on the ultrastructure of human endometrial glandular epithelium was studied at LH+4 (embryo transfer time). In this research endometrial biopsies were obtained from fertile women as well as infertile women who had undergone this protocol at LH+4. Quantitative and qualitative studies on endometrial glandular epithelium was performed by transmission electron microscopy (TEM) and morphometry and the results were statistically compared between the two groups. Qualitative results revealed presence of nuclear channel system (NCS), sub vacuole of glycogen and giant mitochondria (GM) in both groups. Similarly, in quantitative analysis, the volume fractions (Vv) of glycogen, mitochondria and rough endoplasmic reticulum to cell and also the Vv of euchromatin to nucleus were statistically not different (P> 0.05). These results suggest that ovulation induction by GnRH analogue, HMG and HCG are not associated with advanced endometrial development and consequently, embryo transfer at this stage (before advanced endometrial development which occurs normally at LH+7 to LH+10) may cause a lower rate of implantation.

Keywords: Endometrial development, GnRH agonist, Human menopausal genadotropin, HCG, Morphometry


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References
  1. Keye W., Chang R., Rebar R., et al. Infertility evaluation and treatment. W.B. Saunders company,Philadelphia. 1995: 115-26.
  2. Landgren B., Johannisson E. A new method to study the process of implantation of a human blastocysis in vitro. Fertil Steril. 1996; 65(5): 1967-70.
  3. Can A., Tekebiogbu M. Biberoglu K. Structure of premenstrual endometrium in HMG/ HCG induced an ovulatory women. Eur J Obs Gyn Reprod Biol. 1991; 42(2): 119-24.
  4. Csemiczky G., Wramsly H., Johannisson E., et al. Importance of endometrial quality in women with tubal infertility during a natural menstrual cycle for the outcome of IVF treatment. J Assist Reprod Genet. 1998; 15(2): 55-61.
  5. Bourgain C., Smitz J., Camus M. Human endometrial maturation is markedly improved after luteal supplementation of GnRH/HMG stimulated cycles. Hum Reprod. 1994; 9(1): 32-40.
  6. Garcia J. E., Acosta A., Hsiu J., et al. Advanced endomtrial maturation after ovulation with HMG/HCG for in vitro Fertilization. Fertil Steril. 1984; 41(1): 31-5.
  7. Ami M., Geslevich Y., Matilsky M., et al. Exogenous estrogen therapy concurrent with clomiphene citrate lack of effect on serum sex hormone levels and endometrial thickness. Obs Gyn. 1994; 37(3): 180-2.
  8. Ghaffari M. Morphorogy of human endometrial epithelial cells in vitro and its application for human implantation. J Shahid Sadoughi University Med Sci Health. 2000; 8(3).
  9. Li T., Rogers A., Dockery P., et al. A new method of histologic dating of human endometrium in the luteal phase. Fertil Steril. 1988; 50(1): 52-60.
  10. Sarani S., Ghaffari Novin M., Warren M., et al. Morphological evidence for the “implantation window” in human luminal endometrium. Hum Reprod. 1999; 14(12): 3101-6.
  11. Dockery P., Rogers A. The effect of steroids on the fine structure of the endometrium. Bailliere’s Clin Obs Gyn. 1989; 3(2): 227-47.
  12. Borell U., Nilsson O., Westman A. The cyclic changed occurring in the epithelium lining the endometrial glands. Acta Obs Gyn Scandinav. 1959; 38: 364-76.
  13. Bergh P., Navot D. The impact of embryonic development and endometrial maturity on the timing of implantation. Fertil Steril. 1992; 58(3): 537-42.
  14. Nikas G., Develioglu O., Toner J., et al. Endometrial pinopodes indicates a shift in the window of receptivity in IVF cycles. Hum Reprod. 1999; 14(3): 787-92.
  15. Macrow P., Li T., Seif M., et al. Endometrial structures after super ovulation: a prospective controlled study. Fertil Steril. 1994; 61(4): 696-9.
  16. Rogers P. A., Ploson D., Murphy C. R., et al. Correlation of endomtrial histology, morphometry and ultrasound appearance after different stimulation protocols for IVF. Fertil Steril. 1991; 55(3): 583-7.
  17. Bonhoff A., Naether O., Johannisson E. Effects of clomiphene citrate stimulation on endometrial structure in infertile women. Hum Reprod. 1996; 11(4): 844-9.
  18. Li T., Warren M. Ovulation induction for luteal phase defect and luteal phase defect after ovulation induction. Bailliere’s Clin Obs Gyn. 1993; 7(2): 389-419.
  19. Dockery P., Pritchard K., Taylor A., et al. The fine structure of the human endometrial glandular epithelium in cases of unexplained infertility: a morphometric study. Hum Reprod. 1993; 8(5): 667-73.
  20. Dockery P., Pritchard K., Warren M.,et al. Changes in nuclear morphology in the human endometrial glandular epithelium in women with unexplained infertility. Hum Reprod. 1996; 11(10): 2251-6.
  21. Shoham Z., Howles C., Jacobs H. Female infertility therapy current practice. Martin Dunitz Ltd. London. 1999; 3-11, 75-90, 126-33, 215-6, 325-37, 393-407.
  22. Miannay E., Boutemy J., Leroy Billiard M., et al. The possible endometrial risk of ovarian stimulation. J Obs Gyn Biol Reprod Paris. 1994; 23(1): 35-8.



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