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Skip Navigation LinksJRI > Archive > July-September 2002, Volume 3, Issue 3 > Effect of nicotine on sperm of normospermic men: modulations by made antioxidants



Volume 3, Issue 3, Number 11 / July-September
(pages 11-22)


Effect of nicotine on sperm of normospermic men: modulations by made antioxidants




 Corresponding Author
Biology Department, Faculty of Sciences, Shahr Kord University, Shahr-e-Kord, Iran

Biology Department, Faculty of Zeology, Panjanb University, Chandigar, India


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Abstract
Nicotine is one of the major components of cigarette smoke, which have harmful effects like tobacco in human body. Aim of this study is to determine the effect of nicotine on sperm cells. For this purpose we studied the effect of 0.5 & 1mM of nicotine on extent of lipoperoxidation (LPO), balance between oxidized (GSSG) and reduced glutathione (GSH), glutathione S-transferase (GST) activity, and extent of DNA fragmentation with antioxidant interactions in spermatozoa of normospermic men. Vitamin C, glutathione (GSH), and trolox (a water-soluble analog of Vitamin E) were used as antioxidants. Nicotine treatments (0.5 & 1mM) could elevate the level of thiobarbituric acid reactive substances (TBARS) by 51.50% & 78% (P<0.01&P<0.001). Antioxidants could diminish the TBARS level, amongst trolox was shown better result, but Vitamin C acted as a pro-oxidant when ferrous ions were added to the medium. It was found that the glutathione redox ratio (GSH/GSSG) decreased upon nicotine additions by 49.20% & 60.30% (P<0.01). The GST activity also was increased by about 34.01% & 57.19%, in a significant manner respectively. The comet assay results revealed that nicotine could induce severe double-stranded breaks in sperm DNA ladder. Elevated level of TBARS and decreased GSH/GSSG demonstrated a severe peroxidation on sperm membrane lipids through oxygen-derived free radicals. The upgraded activity of GST is an indicator of propagation of LPO in sperm. Collectively, these changes are able to cause inactivation in sperm leading to infertility.

Keywords: Sperm, Nicotine, Free radical, Antioxidant, Glutathione-S, Transferase, Comet assay, Lipid peroxidation


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References
  1. Kulikauskas B., Blaustain D., Ablin R.J. Cigarette smoking and its possible effects on sperm. Fertil Steril. 1985; 44: 526- 8.
  2. Kavitharaj N.K., Vijaymmal P.L. Nicotine administration induced changes in the gonadal function in male rats. Pharmacol. 1999; 58: 2- 7.
  3. Gorrod J.W., Wahren J. Nicotine and related alkaloids: absorption, distribution, metabolism and excretion.Published by Chapman & Hall, London, Glasgow, New York, Tokyo. 1993.
  4. Racowsky C., Kaufman M.L. Nuclear degeneration and meiotic aberrations observed in human oocytes matured in vitro: analysis by light microscopy. Fertil Steril. 1992; 58: 8759- 5.
  5. Weisberg E. Smoking and reproductive health. Clin Reprod Fertil. 1985; 3: 175- 86.
  6. Kasson B.G., Hsueh A.J. Nicotinic cholinergic agonists inhibit androgen biosynthesis by cultural rat testicular cells. Endocrinol. 117: 1874- 80.
  7. Blackburn C.W., Peterson C.A., Hales H.A, et al. Nicotine but not cotinine, has a direct toxic effect on ovarian function in the immature gonadotropin-stimulated rats. Reprod Toxicol. 1996; 814: 325- 31.
  8. Cope G.F. The in vitro effects of nicotine and cotinine on sperm motility. Hum Reprod. 1998; 13: 777- 8.
  9. Sharma R.K., Agarwal A. Role of reactive oxygen species in male infertility. Urol. 1996; 48(6): 835- 50.
  10. Aitken R.J. Free radicals, Lipid peroxidation and sperm function. Reprod Fertil Dev. 1995; 7:65-70.
  11. Fraga C.G., Motchnik P.A., Wyrobek A.J., et al. Smoking and low antioxidant levels increase oxidative to sperm DNA. Mut Res. 1996; 35: 199-203.
  12. Reddy A., Sood A., Rust P.F., et al. The effects of nicotine on in vitro sperm motion characteristics. JAssist Reprod Genet. 1995; 12: 317- 23.
  13. Buege J.A., Steven A.V. Methods in enzymology (biomembranes; part C: biological oxidants), Published by Academic Press. 1978; 302- 10.
  14. Sedlak J., Lindsay R.H. Estimation of total, protein bound and non-protein bound sulphydryl groups in tissues with Ell man’s reagent. Anal Biochem. 1968; 25: 192- 205.
  15. Habig W.H., Pabst M.J., Jakoby S. Glutathione S-transferase: the first enzymatic step in mercapturic acid formation. J Biol Chem. 1974; 219: 7130- 9.
  16. Singh N.P., McCoy M.T., Tice R.R., et al. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res. 1988; 175: 184- 91.
  17. Pasqualotto F.F., Sharma R.K., Nelson D.R., et al. Relationship between oxidative stress, semen characteristics and clinical diagnosis in men undergoing infertility investigation. Fertil Steril. 2000; 73: 459- 64.
  18. Kobayashi H., Gil-Guzman E., Mahran A.M., et al. Quality control of reactive oxygen species measurement by Iuminal-dependent chemiluminescence assay. J Androl. 2001; 22: 568- 74.
  19. Ernster L. Lipid peroxidation in biological membranes: mechanisms and implications, in: Active oxygen, lipid peroxides and antioxidants. Published by CRC Press, Boca Raton. 1993; 1-38.
  20. Yuli I., Wilbrandt W., Shinitzky M. Glucose transport through cell membrane of modified lipid fluidity.Biochemistry. 1981; 20: 4250- 7
  21. Aitken R.J., Buckingham D., West K., Wu F.C., et al. Differential contribution of leucocytes and spermatozoa to high level of reactive oxygen species on human spermatozoa. J Reprod Fertil. 1992; 98: 257- 65.
  22. Aitken R.j., Clarkson J.S. Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa. J Reprod Fertil. 1987; 81: 459- 69.
  23. Wetscher G.J., Bagchi M., Bagchi D., et al. Free radical production in nicotine-treated pancreatic tissue.Free Radic Biol Med. 1995; 18: 877- 82.
  24. Reddy A., Sood A., Rust P.F., et al. The effects of nicotine on in vitro sperm motion characteristics. J Assist Reprod Genet. 1995; 12: 217- 23.
  25. Pekarsky A., Varn E., Mathur R.S., et al. Effects of nicotine on sperm attachment and penetration of zona-free hamster eggs. Arch Andro. 1995; 34: 77- 82.
  26. Barclay L.R.C., Vinqvist M.R. Membrane peroxidation: inhibiting effects of water-soluble antioxidants on phospholipids of different charge type. Free Radic Biol Med. 1994; 16: 779- 88.
  27. Gutteridge J.M.C. Antioxidants, nutritional supplements and life – threatening diseases. Br J Biomed Sci. 1994; 51:288- 95.
  28. Mammoto A., Masumoto N., Tahra M., et al. Reactive oxygen species block sperm-egg fusion via oxidation of sperm sufhydryl proteins in mice. Biol Repod. 1996; 55: 1063- 8.
  29. Nemeth I., Orvos H., Boda D. Blood glutathione redoxstatus in gestational hypertension. Free Radic Biol Med. 2001; 715-21.
  30. Gopalakrishnan B., Aravinda S., Pawsh C.H., et al. Studies on glutathion S-transferases important for sperm function: evidence of catalytic activity-independent functions. Biochem J. 1998; 329: 231- 41.
  31. Hughes C.M., lewis S.E.M., Mekelvey-Martin V.J., et al. A comparison of baseline and infertile men, using a modified comet assay. Mol Hum Reprod. 1996; 2: 613- 9.
  32. Barroso G., Morshedi M., Oehninger S. Analysis of DNA fragmentation, plasma membrane translocation of phosphatidylserine and oxidative stress in human spermatozoa. Hum Reprod. 2000; 15: 1338- 44.



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