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Nosratollah Zarghami Corresponding Author
- Biochemistry Department, Tabriz Univarsity of Medical Sciences, Tabriz, Iran
Mohammad Rahbani Nobar
- Biochemistry Department, Tabriz Univarsity of Medical Sciences, Tabriz, Iran
Jafar Nouroozzadeh
- Clinical Biochemistry Department, Faculty of Medicine, Urmia Medical Sciences University, Urmia, Iran
Yaghoub Deldar
- IVF Center of Kosar Hospital, Faculty of Medicine, Urmia Medical Sciences University, Urmia, Iran
Mohammad Nouri
- Biochemistry Department, Tabriz Univarsity of Medical Sciences, Tabriz, Iran
Maerefat Ghaffari
1- Biochemistry Department, Tabriz Univarsity of Medical Sciences, Tehran, Iran
2- Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

Received: 7/1/2002 Accepted: 7/1/2002 - Publisher : Avicenna Research Institute

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Abstract

Sperm DNA oxidative damage due to free radicals is a major contributing factor in a variety of human diseases, including male infertility. The aim of the present study was to compare the levels of sperm DNA oxidative damage in infertile (according to WHO criteria) and fertile men. Semen samples were obtained after 3 to 7 days of abstinence from 25 infertile and 23 fertile men who referred to infertility Center of Tabriz University of Medical Sciences. After semen analysis, sperm cells were separated from seminal fluid by percoll gradient centrifugation and kept at –20°C for next further analysis. Sperm DNA was extracted and its concentration and purity were determined by UV-spectrophotometer. DNA damage was studied by DNA Chromatography-mass spectrometry (GC/MS). Analysis of 8 hydroxy Guanine (8-OHG) as a marker of DNA oxidative damage by GC/MS was shown that rate of basal DNA oxidative damage in infertile group was 100-fold greater than fertile group. There was also correlation between semen parameter (morphology, motility and sperm count) and DNA oxidative damage. Result of this study showed that elevation of sperm DNA oxidative damage could be one of possible reasons for male infertility.


Keywords: Oxidative DNA damage, Free radical, Antioxidant, 8-hydroxy guanine, Infertility


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References

  1. Mosher, W.D and W.F. Pratt; “Fecundity and Infertility in the United Stated: Incidence and Trends”, Fertil Steril. 1991; 56:192.
  2. Kodama H., Yamaguchi R., Fukuda J., et al. Increased oxidative deoxyribonucleic acid damage in the spermatozoa of infertile male patients. Fertil Steril. 1997; 68(3): 519- 24.
  3. Halliwell B., Guttering J.M. Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem J. 1984; 219: 1- 14.
  4. Quzman E.G, Ollero M, Lopez M.C. Differential production of reactive oxygen species by subset of human spermatozoa at different stages of maturation. Hum Reprod 2001, 16(8): 1922- 30.
  5. Hendin EN, Kolettis PN, Sharma PK. Varicocele is associated with elevated spermatozoa active oxygen species production and diminished seminal plasma antioxidant capacity. J Urol 1999, 161(6): 1831-4.
  6. Aitken RJ. The amorosol lecture. The spermatozoon: a cell in crisis? J Reprod Fertil 1999, 115(1): 1-7.
  7. Ollero M, Enrique G, Lopez M.C. Characterization of subset of human spermatozoa at different stage of maturation: implication treatment of male infertility. In the diagnosis and treatment of male infertility. Hum Reprod. 2001, 16(9): 1912- 21.
  8. Rehman A., Jenner A., Halliwell B: Gas chromatography-mass spectrometry analysis of DNA:optimization of protocols for isolation and analysis of DNA from human blood. Methods in Enzymol. 2000; 319: 401- 17.
  9. Fraga CG, Shigenaga MK, Park JW, Degan P, Ames B. Oxidative damage to DNA during aging: 8-hydroxy,2-deoxyguanosine in rat organ DNA and urine. Proc Natl Acad Sci USA 1991; 87: 4533- 7.
  10. Fraga CG, Motchnik PA, Shigm. Enaga MK, Helbrock JH, Jacob RA, Ames B. Ascorbic acid protects against endogenous oxidative DNA damage in human sperm. Proc Natl Acad Sci USA. 1991; 88: 11003- 6.
  11. Shen HM, Chia SE, Ong CN. Evaluation of oxidative DNA damage in human sperm and its association with male infertility. J Androl. 1999; 20(6): 718- 23.
  12. Shen H, Ong C. Detection of oxidative DNA damage in human sperm and its association with sperm function and male infertility. Free Radic Biol Med. 2000; 28(4): 529- 36.
  13. Zenzes T.M., Bielecki R., reed E. Detection of benzopyrene diolepoxide DNA adducts in sperm of men exposed to cigarette smoke. Fertil Steril. 1999; 72(2): 330- 9.

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