- Fatemeh Haji- Maghsoudi
 - - Department of Biology and Anatomy, Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
- Mohammad Ali Khalili
- - Department of Biology and Anatomy, Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
- Ali Karimzade
- - Department o f Internal Medicine, Afzalipour Educational Hospital, Kerman University of Medical Sciences, Kerman, Iran
Received: 10/21/2009 Accepted: 1/18/2010 - Publisher : Avicenna Research Institute |
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Abstract
Introduction: Ecstasy drug abuse is relatively common among the youth undergoing psychosocial stresses. Categorized as a stimulant drug, ecstasy has nervous, cardiac and immunological side-effects. The endocrine system is not spared from the harmful effects of the drug and it could influence ovulation by exerting its effects on the hypothalamic-hypophyseal-gonadal axis. The aim of this study was to evaluate the effects of ecstasy on the quality of oocytes and on fertilization rate in mice. Materials and Methods: Thirty female NMRI mice were divided into three groups, after checking for their estrous cycles. At the beginning of each cycle, ecstasy was administered intraperitoneally to the animals in groups A (a minimum dose of 5 mg/kg/day) and B (a maximum dose of 20 mg/kg/day). At the end of the second day, 10 IU PMSG, and at the end of the 4th day, 10 IU HCG were administered intra-peritoneally. The mice in group C underwent the same procedures except receiving ecstasy. All the mice were sacrificed on the fifth day to aspirate their oocytes for further evaluation. In-vitro fertilization was done using mature or MII oocytes and fertilization was checked after 5 hours. The results were later statistically analyzed. Results: The number of retrieved oocytes was similar between the experimental and control groups. However, the rate of mature oocytes (MII) was significantly higher (p = 0.0001) in the controls (31.2%) than the mice in groups A (15.2%) and B (12.8%). Moreover, fertilization rate was significantly higher in the controls than the experi-mental groups. Conclusion: Administration of Ecstasy in mice can affect both oocyte maturity and the fertilization potential of oocytes. The overall effects of ecstasy on embryo devel-opment and implantation needs further studies to reach a definitive conclusion.
Keywords:
Ecstasy, Oocyte, Ovary, Fertilization, In Vitro fertilization, Beta-keto-3,4-methylenedioxymethamphetamine (MDMA), Fertility, Methamphetamine To cite this article:References
- Sprague JE, Banks ML, Cook VJ, Mills EM. Hypothalamic-pituitary-thyroid axis and sympathetic nervous system involvement in hyperthermia induced by 3,4-methylenedioxymethamphetamine (Ecstasy). J Pharmacol Exp Ther. 2003;305(1):159-66. [PubMed]
- Gerra G, Bassignana S, Zaimovic A, Moi G, Bussandri M, Caccavari R, et al. Hypothalamic-pituitary-adrenal axis responses to stress in subjects with 3,4 - methylenedioxy - methamphetamine ('ecstasy') use history: correlation with dopamine receptor sensitivity. Psychiatry Res. 2003;120(2):115-24. [PubMed]
- Hesami Z, Khatamsaz S, Mokhtari M. [The effects of ecstasy on pituitary-gonadal axis and spermatogenesis in mature male rats]. Tabib -e- Shargh. 2008;10(3):207-18. Persian. [Abstract]
- Faria R, Magalhães A, Monteiro PR, Gomes-Da-Silva J, Amélia Tavares M, Summavielle T. MDMA in adolescent male rats: decreased serotonin in the amygdala and behavioral effects in the elevated plus-maze test. Ann N Y Acad Sci. 2006;1074:643-9. [PubMed]
- Gelder M, Mayou R, Geddes J. Oxford core texts psychiatry. 2nd ed. Purafkari N, translator. Tehran: Golban medical publication; 2002. p. 202-4.
- Mas M, Farré M, de la Torre R, Roset PN, Ortuño J, Segura J, et al. Cardiovascular and neuroendocrine effects and pharmacokinetics of 3, 4-methylenedioxymethamphetamine in humans. J Pharmacol Exp Ther. 1999;290(1):136-45. [PubMed]
- Koesters SC, Rogers PD, Rajasingham CR. MDMA ('ecstasy') and other 'club drugs'. The new epidemic. Pediatr Clin North Am. 2002;49(2):415-33. Review. [PubMed]
- Cole JC, Sumnall HR. Altered states: the clinical effects of Ecstasy. Pharmacol Ther. 2003;98(1):35-58. Review. [PubMed]
- Oktaei H. [Gut Pharmacology]. 13th ed. Tehran: Hayan & Shaheed Beheshti University of Medical Science; 1992. p. 268-70. Persian.
- Galineau L, Belzung C, Kodas E, Bodard S, Guilloteau D, Chalon S. Prenatal 3,4-methylenedioxy-methamphetamine (ecstasy) exposure induces long-term alterations in the dopaminergic and serotonergic functions in the rat. Brain Res Dev Brain Res. 2005;154(2):165-76. [PubMed]
- Won L, Bubula N, Heller A. Fetal exposure to (+/-) methylenedioxymethamphetamine in utero en-hances the development and metabolism of serotonergic neurons in three-dimensional reaggregate tissue culture. Brain Res Dev Brain Res. 2002; 137(1):67-73. [PubMed]
- Knigge U, Wollesen F, Dejgaard A, Larsen K, Christiansen PM. Modulation of basal and LRH-stimulated gonadotrophin secretion by histamine in normal men. Neuroendocrinology. 1984;38(2):93-6. [PubMed]
- Tutian Z, Fazelipur S, Shadkhast M. [Study of histologic & histometric change on ovarian cortex after administration of morphine in mice]. Iran Vet Med J .2008;4(4):90-6. Persian.
- Sharpe RM, McNeilly AS. The effect of induced hyperprolactinaemia on Leydig cell function and LH-induced loss of LH-receptors in the rat testis. Mol Cell Endocrinol. 1979;16(1):19-27. [PubMed]
- Malberg JE, Seiden LS. Small changes in ambient temperature cause large changes in 3,4-methylene-dioxymethamphetamine (MDMA)-induced serotonin neurotoxicity and core body temperature in the rat. J Neurosci. 1998;18(13):5086-94. [PubMed]
- Nash JF Jr, Meltzer HY, Gudelsky GA. Elevation of serum prolactin and corticosterone concentra-tions in the rat after the administration of 3,4-methylenedioxymethamphetamine. J Pharmacol Exp Ther. 1988;245(3):873-9. [PubMed]
- Scearce-Levie K, Viswanathan SS, Hen R. Locomotor response to MDMA is attenuated in knockout mice lacking the 5-HT1B receptor. Psychopharmacology (Berl). 1999;141(2):154-61. [PubMed]
- Sahraei H, Kaka Gh, Ghoshooni H, Shams Lahijani M, Ramezani M. [Effect of oral morphine administration on fertility of Balb/c mice]. J Reprod Infertil. 2002;3(3):4-10. Persian. [Abstract]
- Simantov R, Tauber M. The abused drug MDMA (Ecstasy) induces programmed death of human serotonergic cells. FASEB J. 1997;11(2):141-6. [PubMed]