%0 Journal Article %A R Aboutorabi %A E Behzadi %A MJ Sadegh %A SP Fatehi %A S Semsarzadeh %A Y Zarrin %A M Kazemi %A L Rafiee %A FS Mostafavi %B Journal of Reproduction & Infertility %C Tehran, Iran %D 2018 %T The Study of Association Between Polymorphism of TNF-α Gene’s Promoter Region and Recurrent Pregnancy Loss %J JRI %> https://www.jri.ir/documents/fullpaper/en/30033.pdf %U https://www.jri.ir/article/30033 %K %P 211-219 %V 19 %N 4 %G English %I Avicenna Research Institute %( Avicenna Research Institute %@ 2251-676X %X

Background: According to the literature review, polymorphisms of tumor necrosis factor alpha’s (TNF-α) promoter region are probably the genetic risk factors of recurrent pregnancy loss. This study has investigated five single nucleotide polymorphisms in the TNF-α gene’s promoter region to evaluate their relationship with recurrent pregnancy loss disorder.
Methods: Blood samples were taken from 65 women with recurrent pregnancy loss (Case group) and 65 healthy women with a history of successful pregnancy (Control group). Polymerase chain reaction with high resolution melting (PCR-HRM) analysis was done to determine the promoter region of -308G/A, -850T/C, -238G/A, -1031T/C and -863A/C TNF-α polymorphisms. The data were assessed using logistic regression models. P values less than 0.05 were considered statistically significant.
Results: Significant associations were found between recurrent pregnancy loss and -863C/A (p=0.000), -308G/A (p=0.045), and -238G/A (p=0.034) polymorphisms. TNF-α polymorphisms of -863C and -238G may be susceptible factors of recurrent pregnancy loss cases. The -308G polymorphism has an important role in maintaining pregnancy.
Conclusion: The -863C/A and -238G/A TNF-α polymorphisms are possible genetic risk factors of recurrent pregnancy loss and might be its predictive markers.