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23926553
New Hopes for the Treatment of Primary Ovarian Insufficiency/ Premature Ovarian Failure
01
3
https://www.jri.ir/article/577
https://www.jri.ir/documents/fullpaper/en/577.pdf
Mohammad RezaSadeghiEditor-in-chief, Tehran, Iran محمدرضاصادقی77
en
23926554
The Concepts and Consequences of Early Ovarian Ageing: A Caveat to Women’s Health
Apparent rise in the incidence of infertility in females and the trend shifting towards delayed child bearing brought up the concept of ovarian ageing. Women in their early thirties show poor ovarian reserve which is an entity named as early ovarian ageing. Early ovarian ageing is mostly genetically determined, but acquired modifiable factors like smoking, or ovarian surgery have some roles. Infertility and subfertility are the only clinical recognizable sequelae in the early ovarian ageing. The worrisome fact is that the outcome of assisted reproductive techniques is also not that much encouraging. Even if ovarian priming with DHEA has raised hope in the assisted reproductive techniques for these patients, but more randomized trials are needed to support this. Screening of these women with antimullerian hormone, antral follicle count and genetic analysis may be useful for recommendation at appropriate biological time regarding conception or fertility preservation.
Assisted Reproductive Techniques, Early ovarian ageing, Infertility, Oocyte
03
8
https://www.jri.ir/article/511
https://www.jri.ir/documents/fullpaper/en/511.pdf
PandaSubratDepartment of Obstetrics and Gynaecology, NEIGRIHMS, Shillong, IndiaPandaSubrat1073
SinghA. SantaDepartment of Obstetrics and Gynaecology, NEIGRIHMS, Shillong, IndiaSinghA. Santa1074
JhaVandanaDepartment of Obstetrics and Gynaecology, NEIGRIHMS, Shillong, IndiaJhaVandanadr.vandanajha@gmail.com1075
en
23926555
The In vitro Fertilization of Ovine Oocytes in the Presence of Oviductal Cells and its Effect on the Expression of Zygote Arrest 1 (Zar1) and Subsequent Embryonic Development
Background: The cells of mammalian female reproductive tract have been widely used for in vitro fertilization (IVF). This study was designed to study the effects of oviductal epithelial cells (OECs) and their conditioned medium during IVF on subsequent embryonic development and the relative abundance of zygote arrest 1 (Zar1) transcript in ovine zygotes.
Methods: The in vitro matured ovine oocytes were randomly fertilized in the following culture conditions: I) SOFaaBSA+20% sheep serum (control), II) SOFaa BSA+20% sheep serum (50 µl) in the presence of OECs, III) SOFaaBSA+20% sheep serum (100 µl) in the presence of OECs, and IV) OECs conditioned medium (CM). Sigma Stat (Version 2.0) software and one-way ANOVA were considered for statistical analysis. A p<0.05 was considered statistically significant.
Results: The cleavage, blastocyst, and hatched blastocyst rates in OECs and CM groups were significantly lower than the control group (p<0.01). In co-cultured groups, the application of two different volumes of IVF medium showed no difference in embryonic developmental indices. The Zar1 gene expression in zygotes produced in the presence of OECs was significantly higher than those produced in the control and CM groups (p<0.05).
Conclusion: Neither the presence of oviductal epithelial cells nor their conditioned medium could improve the developmental potential of ovine embryos during IVF. Moreover, no relationship was observed between the relative abundance of Zar1 transcript in zygotes produced in different conditions and the corresponding subsequent embryonic development.
Epithelial oviductal cells, IVF, Ovine zygote, Zygote arrest 1
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17
https://www.jri.ir/article/516
https://www.jri.ir/documents/fullpaper/en/516.pdf
AbolfazlShiraziDepartment of Gametes and Cloning, Research Institute of Animal Embryo Technology, Shahrekord University, Shahr-e-Kord, Iranابوالفضلشيرازیa.shirazi@avicenna.ac.ir795
EhsanMotaghiDepartment of Gametes and Cloning, Research Institute of Animal Embryo Technology, Shahrekord University, Shahr-e-Kord, IranEhsanMotaghi1095
en
23926556
In vitro Culture of Human Testicular Stem Cells on Feeder-Free Condition
Background: Spermatogonial stem cells are subpopulation of spermatogonial cells in testis tissue that support beginning and maintenance of spermatogenesis. Ubiquitin carboxy-terminal hydrolase L1 (UCHL1) could be a specific marker for identification of spermatogonial stem cells including spermatogonial sperm cells (SSCs) in testis tissue and during the culture; therefore we undertook this study to culture these human testicular stem cells (hTSCs) in vitro and approved the presence of human testicular stem cells (hTSCs) by UCHL1, also known as PGP9.5.
Methods: Enzymatic digestion of human testicular biopsies was done by collagenase IV (4 mg/ml) and trypsin (0.25%). Differential plating of testicular cells in DMEM/F12 and 10% FBS was applied for 16 hr. Floating cells were collected and transferred onto laminin-coated plates with Stem-Pro 34 media supplemented with growth factors of GDNF, bFGF, EGF and LIF to support self-renewal divisions; testicular stem cell clusters were passaged every 14 days for two months. Spermatogonial cells propagation was studied through Expression of UCHL1 in testis tissue and the entire testicular stem cell culture.
Results: Testicular stem cell clusters from 10 patients with obstructive azoospermia were cultured on laminin-coated plates and subsequently propagated for two months. The average of harvested viable cells was approximately 89.6%. UCHL1 was expressed as specific marker in testicular stem cells entire the culture.
Conclusion: Human testicular stem cells could be obtained from human testicular tissue by a simple digestion, culturing and propagation method for long-term in vitro conditions. Propagation of these cells approved by specific marker UCHL1, during the culture period.
Human testicular stem cells, Laminin, Long term culture, UCHL1
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23
https://www.jri.ir/article/517
https://www.jri.ir/documents/fullpaper/en/517.pdf
ZeinabPiravarDepartment of Biology, Science and Research Branch, Islamic Azad University, Tehran, IranZeinabPiravar1096
MahmoodJeddi-TehraniMonoclonal Antibody Research Center, Avicenna Research Institute (ACECR), Tehran, Iranمحمود جدیتهرانی54
Mohammad RezaSadeghiMonoclonal Antibody Research Center, Avicenna Research Institute (ACECR), Tehran, Iran محمدرضاصادقی77
ArashMohazabReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iranآرشمهذب906
AkramEidiDepartment of Biology, Science and Research Branch, Islamic Azad University, Tehran, IranAkramEidi1097
Mohammad MehdiAkhondiReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iranمحمدمهدیآخوندیakhondi@avicenna.ac.ir21
en
23926557
Correlation of the Day 3 FSH/LH Ratio and LH Concentration in Predicting IVF Outcome
Background: This study was undertaken to evaluate the role of day 3 FSH/LH ratio and day 3 LH level as predictors of IVF cycle outcomes.
Methods: This prospective observational study was undertaken in the IVF and Reproductive Biology Centre and Lok Nayak Hospital, affiliated to Maulana Azad Medical College, in New Delhi, India. The study included 105 women who underwent controlled ovarian hyperstimulation for in vitro fertilization. Characteristics of IVF cycles and outcomes were studied in patient subgroups based on day 3 FSH/LH ratio (<2 and ≥2) and day 3 LH levels (>3 and ≤3 mIU/ml). The student t-test, Bartlett's test, chi-squred and Fisher's exact test, and linear regression model were used for data analysis. A p-value less than 0.05 was considered as statistically significant.
Results: Women with an elevated FSH/LH ratio ≥2 (n=31) required higher doses of gonadotrophins (3019.34 vs. 2482.43 IU). The outcome of IVF was poor in these patients and they had fewer number of mature follicles (>16 mm) (5.44 vs. 6.09), less E2/mature follicle ratio (4.65 vs. 6.36), fewer retrieved oocytes (6.67 vs. 9.09) and fewer pregnancy rates (11.1% vs. 33.8%). On the other hand, patients with low basal LH levels (≤3 mIU/ml) did not differ significantly in terms of response to controlled ovarian hyperstimulation except for fewer number of retrieved oocytes (7.33 vs. 7.91) but there was a trend towards poor pregnancy rates (7.33 vs. 7.91) but there was a trend towards poor pregnancy rates as compared to subgroup with LH levels >3 mIU/ml.
Conclusion: Elevated day 3 FSH/LH ratio is associated with inferior outcome in IVF treatment cycles and it could be used as an additional predictor of decreased ovarian reserve.
FSH, IVF outcome, LH, Ovarian hyperstimulation, Ovarian reserve
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29
https://www.jri.ir/article/518
https://www.jri.ir/documents/fullpaper/en/518.pdf
SudhaPrasadIVF and Reproductive Biology Centre, Department of Obstetrics and Gynecology, Maulana Azad Medical College, New Delhi, IndiaSudhaPrasaddrsprasad08@gmail.com1098
TeenaGuptaIVF and Reproductive Biology Centre, Department of Obstetrics and Gynecology, Maulana Azad Medical College, New Delhi, IndiaTeenaGupta1099
AabhaDivyaAll India Institute of Medical Sciences, New Delhi, IndiaAabhaDivya1100
en
23926558
Classical and Molecular Methods for Evaluation of Chlamydia trachomatis Infection in Women with Tubal Factor Infertility
Background: Chlamydia trachomatis is the most reported bacterial sexually transmitted disease, especially among young women worldwide. aim of this study was comparison to evaluate the prevalence of Chlamydia trachomatis infection in woman with tubal infertility by means of PCR and cell culture techniques.
Methods: Fifty-one women with confirmed TFI were enrolled in this study in (avicenna infertility Clinic) between January 2010 and January 2011. Cervical swab and cytobrush specimens were collected from each patient by gynecologists and sent to laboratory in transport media. Detection of Chlamydia trachomatis in samples was performed using PCR and bacteria culture in MacCoy cell line. The data were analyzed by Fisher’s exact test and independent t-test. Statistical significance was established at a p-value <0.05.
Results: A significant relation was observed between increased the age of first intercourse and chlamydial infection. Six (11.7%) samples had positive PCR result, whereas cell culture results were positive in only 2 (3.9%) samples. A significant relation was also identified between the duration of infertility and infection (p<0.05) by PCR versus cell culture method.
Conclusion: The results showed that PCR is a rapid method, compared to cell culture for detecting Chlamydial organism. It also became clear that the age at first intercourse is important to predict the likelihood of Chlamydia trachomatis.
Cell culture, Chlamydia trachomatis, Cytobrush, PCR, Swab
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https://www.jri.ir/article/519
https://www.jri.ir/documents/fullpaper/en/519.pdf
BaharehHajikhaniReproductive Infections Department of Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, IranBaharehHajikhani1101
TayebehMotallebiDepartment of Microbiology, Islamic Azad University, Tehran North Branch, Tehran, IranTayebehMotallebi1102
JamilehNorouziDepartment of Microbiology, Islamic Azad University, Tehran North Branch, Tehran, IranJamilehNorouzi1103
AbbasBahadorDepartment of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, IranAbbasBahador1104
RezvanBagheriReproductive Infections Department of Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, IranRezvanBagheri1105
SoheilaAsgariInternational Campus, Tehran University of Medical Sciences, Kish Island, Iranسهيلاعسگري551
LeiliChamani TabrizReproductive Infections Department of Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iranليليچمني تبریزlchamani@avicenna.ac.ir62
en
23926559
Sexual Behavior of Married Iranian Women, Attending Taleghani Public Health Center
Background: Sexual practices as an important aspect of reproductive health have many physical and psychological effects on people’s lives, there is limited evidence on such practices and their pattern among Iranian women. Hence we aimed to determine different types of sexual practices among 19-45 year old married Iranian women.
Methods: This cross-sectional study was conducted among 200 married women, aged 19-45 years, attending Taleghani Public Health Center for annual gynecologic examination during November 2008 to May 2009 using convenient sampling. The participants were enquired about their experience regarding different types of sex, as well as their views and feelings about such practices, using an anonymous questionnaire.
Results: The mean age of the participants was 34 years. All had ever experienced vaginal sex and 50.9% reported ever experience of other types of sex (non-vaginal), as well. Due to some stigma attached to non-vaginal sexual practices among women in Iran, the feelings of women with regard to different sexual practices were also examined in this paper.
Conclusion: This study showed that non-vaginal sex among women is considerable and because of less favourable views of women towards such practices, it seems that these practices might have psychologically impacts on women’s life. Hence, counseling and educational programs designed for married men and women can include some factual information about different types of sex.
Anal sex, Oral sex, Sexual behavior, Sexual practice, Vaginal sex, Sexual health, Reproductive health
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https://www.jri.ir/article/520
https://www.jri.ir/documents/fullpaper/en/520.pdf
SomayehHashemiReproductive Health Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iranسمیههاشمی780
SeddigheSeddighNursing Midwifery School, Mashhad University of Medical Sciences, Mashad, Iranصدیقهصدیق783
FahimehRamezani TehraniReproductive Health Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iranفهيمهرمضاني تهراني191
Seyed MehdiHasanzadeh KhansariTehran Psychiatric Institute, Tehran University of Medical Sciences, Tehran, IranSeyed MehdiHasanzadeh Khansari1106
NahidKhodakaramiReproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IranناهیدخداکرمیKhodakarami@sbmu.ac.ir782
en
23926560
The Anti-fertility Effects of Acacia nilotica in Male Wistar Rats
<p>Background: A bulk of contraceptives on the market is women-oriented today. The aim of this study was to investigate the effect of a medicinal herb, Acacia nilotica on various parameters of male fertility using a rat model. Methods: Male Wistar rats (n=40) were randomly divided in to two groups. One group received Acacia nilotica, while the other acted as controls. Ten animals from each group were sacrificed after 16 weeks. Treatment was withdrawn for the remaining animals for 8 weeks. Blood was collected for hormonal analysis. The testis was removed for histological examination, while epididymal spermatozoa were retrieved for motility and morphological analysis. The data were analyzed using ANOVA and Bonferroni post hoc test. A value of p<0.05 was considered statistically significant. Results: Sperm motility, progressive motility and sperm concentration significantly decreased in treated animals compared to the controls (p<0.05). Withdrawing the treatment did not restore these parameters (p<0.05). Abnormal sperm morphology significantly increased in both the treated and treatment withdrawn groups when compared to the controls (p<0.05). Testosterone concentrations were significantly lower in the treated group when compared to the controls (p<0.05) and no significant differences were observed between the controls and the treated animals when treatment was withdrawn. Histological observations showed that Acacia nilotica treatment disrupted semeniferous tubule architechture and consequently the spermatogenesis process. Conclusion: These results show that Acacia nilotica severely affects sperm morphology, progressive motility and sperm concentration irreversibly in Wistar rats.</p>
Acacia nilotica, Contraceptives, Family planning, Fertility, Herbal medicine
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https://www.jri.ir/article/521
https://www.jri.ir/documents/fullpaper/en/521.pdf
FanuelLampiaoDepartment of Basic Medical Sciences, Division of Physiology, College of Medicine, Blantyre, MalawiFanuelLampiaoflampiao@medcol.mw1107
en
23926561
Incomplete Cesarean Scar Rupture
<p>Background: Uterine rupture at the site of a previous cesarean scar is an uncommon but catastrophic complication of pregnancy, which is associated with significant maternal and fetal morbidity and mortality. Case Presentation: A 30-year old woman at 24th week of gestation and complaint of pain, contractions and spotting was admitted in Royan Institute in Tehran, Iran. She had a past medical history of an EP and a cesarean section delivery, respectively 4 and 2 years before hospitalization. Herniation of an amniotic membrane into the maternal bladder was found on ultrasound examination. Conclusion: Risk factors of cesarean scar rupture should be considered in women undergoing subsequent pregnancies as they need extra care. Ultrasonography can be used to evaluate women with previous cesarean section to assess the risks of scar rupture during subsequent pregnancies.</p>
Caesarian section, Dehiscence, Rupture, Scar
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https://www.jri.ir/article/522
https://www.jri.ir/documents/fullpaper/en/522.pdf
FiroozehAhmadiDepartment of Reproductive Imaging, Reproductive Biomedicine Research Center, Royan Institute, ACECR, Tehran, IranFiroozehAhmadidr.ahmadi1390@gmail.com1014
ShivaSiahbaziDepartment of Reproductive Imaging, Reproductive Biomedicine Research Center, Royan Institute, ACECR, Tehran, IranShivaSiahbazi1109
FarnazAkhbariDepartment of Reproductive Imaging, Reproductive Biomedicine Research Center, Royan Institute, ACECR, Tehran, IranFarnazAkhbari1108
en
23926562
Endometriosis: A History Written by Aberrant Hoxa10 Gene Expression and Epidermal Growth Factor (EGF) System Polymorphism?
<p>Endometriosis is a gynecologic disorder characterized by the presence of viable, extrauterine endometrial tissue, predominantly on the ovary and pelvic peritoneum (1-3). Typical symptoms consist of pelvic pain, dysmenorrhea, and infertility (1, 2). This condition takes place only in women of menstrual age, can grow or bleed cyclically and may cause adhesions (1, 2). Endometriosis represents a worldwide social disease compromising quality of life (1, 2)....</p>
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https://www.jri.ir/article/523
https://www.jri.ir/documents/fullpaper/en/523.pdf
RaffaellaMormileDivision of Pediatric and Neonatology, Moscati Hospital, Aversa, ItalyRaffaellaMormileraffaellamormile@alice.it1110
GiorgioVittoriDivision of Gynecology, San Carlo di Nancy Hospital, Rome, ItalyGiorgioVittori1111