en
1726-7536
1735-8507
69
2139
288
gregorian
2012
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24
14
1
online
1
fulltext
en
23926558
Classical and Molecular Methods for Evaluation of Chlamydia trachomatis Infection in Women with Tubal Factor Infertility
Background: Chlamydia trachomatis is the most reported bacterial sexually transmitted disease, especially among young women worldwide. aim of this study was comparison to evaluate the prevalence of Chlamydia trachomatis infection in woman with tubal infertility by means of PCR and cell culture techniques.
Methods: Fifty-one women with confirmed TFI were enrolled in this study in (avicenna infertility Clinic) between January 2010 and January 2011. Cervical swab and cytobrush specimens were collected from each patient by gynecologists and sent to laboratory in transport media. Detection of Chlamydia trachomatis in samples was performed using PCR and bacteria culture in MacCoy cell line. The data were analyzed by Fisher’s exact test and independent t-test. Statistical significance was established at a p-value <0.05.
Results: A significant relation was observed between increased the age of first intercourse and chlamydial infection. Six (11.7%) samples had positive PCR result, whereas cell culture results were positive in only 2 (3.9%) samples. A significant relation was also identified between the duration of infertility and infection (p<0.05) by PCR versus cell culture method.
Conclusion: The results showed that PCR is a rapid method, compared to cell culture for detecting Chlamydial organism. It also became clear that the age at first intercourse is important to predict the likelihood of Chlamydia trachomatis.
Cell culture, Chlamydia trachomatis, Cytobrush, PCR, Swab
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https://www.jri.ir/article/519
https://www.jri.ir/documents/fullpaper/en/519.pdf
BaharehHajikhaniReproductive Infections Department of Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, IranBaharehHajikhani1101
TayebehMotallebiDepartment of Microbiology, Islamic Azad University, Tehran North Branch, Tehran, IranTayebehMotallebi1102
JamilehNorouziDepartment of Microbiology, Islamic Azad University, Tehran North Branch, Tehran, IranJamilehNorouzi1103
AbbasBahadorDepartment of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, IranAbbasBahador1104
RezvanBagheriReproductive Infections Department of Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, IranRezvanBagheri1105
SoheilaAsgariInternational Campus, Tehran University of Medical Sciences, Kish Island, Iranسهيلاعسگري551
LeiliChamani TabrizReproductive Infections Department of Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iranليليچمني تبریزlchamani@avicenna.ac.ir62