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24696790
The State of Semen Analysis over Time
01
2
https://www.jri.ir/article/564
https://www.jri.ir/documents/fullpaper/en/564.pdf
Mohammad RezaSadeghiEditor-in-chief, Tehran, Iran محمدرضاصادقی77
en
24696791
Human Sperm DNA Fragmentation and Its Correlation with Conventional Semen Parameters
Background: The initial step in the diagnostic investigation of male infertility has been traditionally based on the conventional seminal profile. However, there are significant limitations regarding its ability to determine the underlying mechanisms that cause the disorder. Sperm DNA fragmentation has emerged as a potential causative factor of reproductive failure and its assessment has been suggested as a useful adjunct to the laboratory methodology of male infertility evaluation, especially before the application of assisted reproduction technology (ART).
Methods: A review of recent bibliography was carried out in PubMed by the use of relevant keywords, in order to evaluate the possible correlation between the conventional seminal parameters and sperm DNA fragmentation assessment as diagnostic tools in male infertility evaluation.
Results: A comprehensive diagnostic approach of male infertility should be based on a combination of diagnostic attributes, derived from the conventional semen analysis, as well as the investigation of genomic integrity testing.
Conclusion: Due to its strong correlation with several aspects of ART procedures and further consequences for the offspring, sperm DNA fragmentation is a parameter worth integrating in routine clinical practice. However, additional large scale studies focusing on specific subgroups of infertile men who may benefit from an efficient therapeutic management based on the optimization of sperm DNA integrity are needed.
Assisted reproduction, Conventional semen parameters, DNA fragmentation
02
15
https://www.jri.ir/article/548
https://www.jri.ir/documents/fullpaper/en/548.pdf
EvangeliniEvgeniLaboratory of Physiology, School of Medicine, Democritus University of Thrace, Thrace, GreeceEvangeliniEvgenilina_evgeni@yahoo.gr1197
KonstantinosCharalabopoulosLaboratory of Physiology, School of Medicine, Democritus University of Thrace, Thrace, GreeceKonstantinosCharalabopoulos1198
ByronAsimakopoulosLaboratory of Physiology, School of Medicine, Democritus University of Thrace, Thrace, GreeceByronAsimakopoulos1199
en
24696155
Relation of Cumulus Cell Status with Single Oocyte Maturity, Fertilization Capability and Patient Age
Background: The production of competent oocytes depends on a bi-directional communication between the oocyte and cumulus cells. The goal of this study was to determine whether simple parameters monitored in cumulus cells from individual human oocytes have any predictive value, and thus correlate with clinically relevant parameters.
Methods: 97 cumulus-oocyte complexes were recovered from 31 patients undergoing ICSI treatment. After the oocytes were denuded, cumulus cell density from individual oocytes was determined. Cells were probed for viability using propidium iodide and for apoptosis by Annexin V staining or by monitoring caspase activity. These parameters were correlated with oocyte status, fertilization ability and patient age (≤29 years old and ≥30 years old). All variables were checked for normal distribution and then compared by Kruskal-Wallis, Mann-Whitney or one-way ANOVA tests.
Results: Mature oocytes were surrounded by more cumulus cells (16073±2595, p=0.026), which were also more viable and less apoptotic than atretic or degenerated oocytes. Mature oocytes that fertilized had higher caspase activity in the surrounding cumulus cells than those that did not fertilize. Younger patients presented lower cumulus cells density (8882±2380 vs. 15036±2143 cells; p=0.034); and cumulus cells had higher apoptosis levels in younger patients than older ones (6775.5±1831.6 RLU vs. 2591±46.5 RLU, p=0.002 for caspase activity).
Conclusion: The data suggests that high density and apoptosis of cumulus cells are promising parameters to indirectly predict individual oocyte status. Although more studies and a larger data set are needed, cumulus cells presented the potential to be used as simple predictors of female fertility and/or ovarian ageing.
Annexin V, Apoptosis, Caspase activity, Cumulus cell, Cumulus-oocyte complex, Female fertility, Oocyte biomarkers, Woman age
15
22
https://www.jri.ir/article/549
https://www.jri.ir/documents/fullpaper/en/549.pdf
BárbaraLourençoBiology of Reproduction and Stem Cell Group, Center for Neuroscience and Cell Biology (CNC), University of Coimbra, Coimbra, PortugalBárbaraLourenço1200
AnaSousaHuman Reproduction Service, University Hospitals of Coimbra, Coimbra, PortugalAnaSousa1201
TeresaAlmeida-SantosFaculty of Medicine, University of Coimbra, Coimbra, PortugalTeresaAlmeida-Santos1202
JoãoRamalho-SantosDepartment of Life Sciences, University of Coimbra, Coimbra, PortugalJoãoRamalho-Santosjramalho@ci.uc.pt1203
en
24696792
Protective Effects of Antioxidants on Sperm Parameters and Seminiferous Tubules Epithelium in High Fat-fed Rats
Background: Prescription of antioxidants might increase the quality of sperm parameters and improve the rate of pregnancy in obese people who suffer from infertility. Therefore, the present study investigated protective effects of vitamin A, E and astaxanthin on sperm parameters and seminiferous tubules epithelium in high-fat diet model.
Methods: Thirty-six numbers of 3 months old albino Wistar rats were divided to control, high-fat diet and high-fat diet with antioxidants groups. After 12 weeks, levels of LDL-C and HDL-C were detected in the groups. Sperm was obtained from the tail of epididymis and its parameters (count, vitality, motility and morphology) were analyzed. Testes were fixed in 10% formalin and after tissue processing, stained with Hematoxylin and Eosine (H&E) for histological evaluation. Data were analyzed by a one-way ANOVA and p<0.05 was considered significant.
Results: Our results indicated that viability, motility and normal morphology of sperm in high-fat diet (HFD) decreased significantly compared to high-fat diet with antioxidant (HFD+A) and the control groups (p<0.05). Also spermatogonium and the number of Sertoli cells increased significantly in HFD+A compared to the control (p<0.05).
Conclusion: As it is shown in our study, application of antioxidants decreased serum triglyceride, cholesterol and HDL-C/LDL-C in high-fat diet model and improved the semen parameters. Therefore, it is suggested that the low quality of sperm can be improved in obese men through antioxidant prescription. Finally, it seems that the antioxidants in obese patients with subfertility or infertility is a new and efficient strategy with few side effects.
Antioxidant, Astaxanthin, High-fat Diet, Spermatogenesis, Testis, Vitamin A, Vitamin C
22
29
https://www.jri.ir/article/550
https://www.jri.ir/documents/fullpaper/en/550.pdf
MotaharehMortazaviAnatomical Sciences Department, Medicine faculty, Hamadan University of Medical Sciences, Hamadan, IranMotaharehMortazavi1204
IrajSalehiNeurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, IranIrajSalehi1205
ZohrehAlizadehAnatomical Sciences Department, Medicine faculty, Hamadan University of Medical Sciences, Hamadan, IranZohrehAlizadeh1206
MehrangizVahabianDepartment of Persian Literature and English Language, Medicine faculty, Hamadan University of Medical Sciences, Hamadan, IranMehrangizVahabian1207
AmanehMohammadi RoushandehResearch Center for Molecular Medicine, Medicine Faculty, Hamadan University of Medical Sciences, Hamadan, IranAmanehMohammadi Roushandeha.mohammadiroshandeh@umsha.ac.ir1217
en
24696793
Sexual and Reproductive Outcomes in Early Stage Cervical Cancer Patients after Excisional Cone as a Fertility-sparing Surgery: An Italian Experience
Background: The purpose of this study was to analyze the quality of life in terms of sexual and reproductive outcome in patients suffering from early stage cervical cancer, submitted to an excisional cone as fertility-sparing treatment.
Methods: A multicenter retrospective analysis about specific dimensions of physical, psychological, reproductive and sexual functions after a cold-knife conization plus pelvic laparoscopic lymphadenectomy was conducted at Division of Gynecologic Oncology, Catholic University of the Sacred Heart, Rome-Italy and at Division of Gynecology, European Institute of Oncology, Milan-Italy. The aim of this study was twofold. It aimed to analyze the quality of life in patients submitted to minimally invasive surgery and to compare these data with radical trachelectomy.
Results: Twenty-three patients with an average age of 30 years decided to participate in this study. After the treatment, all women (100%) had regular menstruation, 7 (30.4%) had increased not invalidating dysmenorrhea; 1 (4.4%) experienced a cervical stenosis; 6 among 10 patients that tried to conceive (60%) obtained one spontaneous pregnancy; 4 more (40%) underwent in vitro fertilization and embryo transfer and only 1 of them (25%) was successful. About sexual assessment, 1 patient (4.4%) had trouble in lubricating, 3 (13%) had anxiety about performance, 6 (26.1%) complained of dyspareunia which was resolved within 3 subsequent months. All patients (100%) obtained a complete psychological and physical recovery.
Conclusion: This study demonstrated preliminary encouraging data about sexual and reproductive outcome after excisional conization. A comparison with trachelectomy surely needs longer follow-ups, more cases and prospective analyses.
Cervical cancer, Conservative approach, Excisional cone, Fertility-sparing surgery, Quality of life
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35
https://www.jri.ir/article/551
https://www.jri.ir/documents/fullpaper/en/551.pdf
FrancescoFanfaniInstitute for Maternal and Child Health, IRCCS "Burlo Garofolo", Trieste, ItalyFrancescoFanfanifrancesco.fanfani@rm.unicatt.it1208
FabioLandoniDivision of Gynecology, European Institute of Oncology, Milan, ItalyFabioLandoni1209
MariaGagliardiDepartment of Obstetrics and Gynecology, Division of Gynecologic Oncology, Catholic University of the Sacred Heart, Rome, ItalyMariaGagliardi1210
AnnaFagottiDivision of Minimally Invasive Gynecological Surgery, Maria Hospital-University of Perugia, Terni, ItalyAnnaFagotti1211
EleonoraPretiDivision of Gynecology, European Institute of Oncology, Milan, ItalyEleonoraPreti1212
MariaMoruzziDepartment of Obstetrics and Gynecology, Division of Gynecologic Oncology, Catholic University of the Sacred Heart, Rome, ItalyMariaMoruzzi1213
GiorgiaMonterossiDepartment of Obstetrics and Gynecology, Division of Gynecologic Oncology, Catholic University of the Sacred Heart, Rome, ItalyGiorgiaMonterossi1214
GiovanniScambiaDepartment of Obstetrics and Gynecology, Division of Gynecologic Oncology, Catholic University of the Sacred Heart, Rome, ItalyGiovanniScambia1215
en
24696794
Comparing the Roles of Sperm Chromatin Integrity and Apoptosis in Intrauterine Insemination Outcomes of Couples with Mild Male and Female Factor Infertility
Background: Intrauterine insemination (IUI) is one of the therapeutic approaches for infertility. The objective of this study was to evaluate DNA integrity and apoptosis role in success of IUI in both mild male and female factor infertility.
Methods: Patients were divided into two groups: M (mild male factor; n=29) and F (female factor; n=31) undergoing single IUI. Ejaculates were analyzed and chromatin quality was assessed using chromomycin A3 (CMA3) staining. In addition, spermatozoal apoptosis was recognized using TUNEL assay. Statistical analyses were done using t-test and Mann Whitney test for sperm apoptosis and sperm chromatin by SPSS. Data were expressed in mean ± SD for variables. P<0.05 was considered statistically significant.
Results: Sperm concentration and progressive motility were higher in F than M group. Sperm with normal morphology were statistically similar in M and F infertile patients (32.7±15.6% vs. 35.5±9.07%, p=0.39). Sperm chromatin immaturity was higher in patients with mild male infertility, when compared with the other group (p<0.01). Also, 32.0±5.6% and 30.8±6.1% of the spermatozoa showed signs of apoptosis in groups M and F, respectively (p=0.49). Very low (3.4%) clinical pregnancy rates were noticed in patients with mild male factor infertility
Conclusion: Defect in sperm motility as well as high rates of DNA damage and apoptosis may be involved with very low rate of pregnancy outcomes in patients with mild male factor infertility. Therefore, it seems the application of IUI may have better outcomes in patients with female infertility compared to mild male factor infertility.
Apoptosis, Infertility, Intrauterine transfusion, Male, Morphology, Sperm motility, Spermatozoa
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41
https://www.jri.ir/article/552
https://www.jri.ir/documents/fullpaper/en/552.pdf
Mohammad AliKhaliliInfertility Research and Clinical Center, Shahid Sadoughi Medical Sciences University, Yazd, Iranمحمد عليخليلي84
SaeedehNazariInfertility Research and Clinical Center, Shahid Sadoughi Medical Sciences University, Yazd, IranSaeedehNazarinazari.saeedeh65@gmail.com1216
RaziehDehghani-FirouzabadiInfertility Research and Clinical Center, Shahid Sadoughi Medical Sciences University, Yazd, IranRaziehDehghani-Firouzabadi1218
AlirezaTalebiInfertility Research and Clinical Center, Shahid Sadoughi Medical Sciences University, Yazd, Iranعليرضاطالبي426
ShekofehBaghazadeh-NaeiniInfertility Center, Isfahan University of Medical Sciences, Isfahan, IranShekofehBaghazadeh-Naeini1219
FatemehSadeghian-NodoshanInfertility Research and Clinical Center, Shahid Sadoughi Medical Sciences University, Yazd, IranFatemehSadeghian-Nodoshan1220
AzamAgha-RahimiInfertility Research and Clinical Center, Shahid Sadoughi Medical Sciences University, Yazd, Iranاعظمآقارحیمی888
en
24695380
Effects of Fenugreek Seed on the Severity and Systemic Symptoms of Dysmenorrhea
Background: Primary dysmenorrhea is a prevalent disorder and its unfavorable effects deteriorates the quality of life in many people across the world. Based on some evidence on the characteristics of fenugreek as a medical plant with anti-inflammatory and analgesic properties, this double-blind, randomized, placebo controlled trial was conducted. The main purpose of the study was to evaluate the effects of fenugreek seeds on the severity of primary dysmenorrhea among students.
Methods: Unmarried Students were randomly assigned to two groups who received fenugreek (n=51) or placebo (n=50). For the first 3 days of menstruation, 23 capsules containing fenugreek seed powder (900 mg) were given to the subjects three times daily for two consecutive menstrual cycles. Pain severity was evaluated using a visual analog scale and systemic symptoms were assessed using a multidimensional verbal scale.
Results: Pain severity at baseline did not differ significantly between the two groups. Pain severity was significantly reduced in both groups after the intervention; however, the fenugreek group experienced significantly larger pain reduction (p<0.001). With respect to the duration of pain, there was no meaningful difference between the two cycles in the placebo group (p=0.07) but in the fenugreek group, the duration of pain decreased between the two cycles (p<0.001). Systemic symptoms of dysmenorrhea (fatigue, headache, nausea, vomiting, lack of energy, syncope) decreased in the fenugreek seed group (p<0.05). No side effects were reported in the fenugreek group.
Conclusion: These data suggest that prescription of fenugreek seed powder during menstruation can reduce the severity of dysmenorrhea.
Dysmenorrhea, Fenugreek, Herbal medicine
41
49
https://www.jri.ir/article/553
https://www.jri.ir/documents/fullpaper/en/553.pdf
SimaYounesyDepartment of Midwifery, School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, IranSimaYounesy1221
SedighehAmiraliakbariDepartment of Midwifery, School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, IranSedighehAmiraliakbariasa_akbari@yahoo.com1222
SomayehEsmaeiliTraditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IranSomayehEsmaeili1223
HamidAlavimajdDepartment of Biostatistics, Faculty of Paramedical, Shahid Beheshti University of Medical Sciences, Tehran, Iranحمیدعلویمجد452
SoheilaNouraeiDepartment of Midwifery, School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, IranSoheilaNouraei1224
en
24696795
Mutation Analysis of Exons 10 and 17a of CFTR Gene in Patients with Cystic Fibrosis in Kermanshah Province, Western Iran
Background: Cystic fibrosis (CF) is the most common genetic disorder with autosomal recessive inheritance among Caucasian populations. So far, more than 1950 different mutations were identified in cystic fibrosis transmembrane conductance regulator (CFTR) gene. CFTR gene has 27 exons. The type and distribution of mutations vary widely among different countries and/or ethnic groups. Therefore, a comprehensive analysis was performed on exon10 and exon17a of CFTR gene in CF patients in the Kermanshah province, western Iran.
Methods: We tested 27 patients admitted to the medical genetics laboratory of Kermanshah University of Medical Sciences. The patients were from different cities of Kermanshah province. All the patients had the clinical signals and two positive sweat tests. After filling agreement forms and questionnaire, the peripheral blood sampling and DNA extraction were done. DNA samples were extracted. PCR and sequencing special PCR were done. Finally analysis of the results with DNA sequencing analysis version 5.2 software was performed.
Results: CFTR mutations analysis identified 4 different mutations in our CF patients. The disease-causing mutations were p.F508del (∆F508) (14.81%), p.S466X (1.85%), and p.T1036I (1.85%). M470V polymorphism with frequency of 74.1% was found in 23 patients (17 homozygous and 6 heterozygous).
Conclusion: Three disease-causing mutations in CF patients in the present study account for approximately 18.51% of mutations. The frequency of p.F508del, the most common mutation was 1618.1% in Iranian population. The results of the present study can be applied for genetic counseling, population screening and prenatal diagnosis.
F508∆, Cystic fibrosis, Direct sequencing, Iran, Kermanshah, M470V, S466X, T1036I
49
57
https://www.jri.ir/article/554
https://www.jri.ir/documents/fullpaper/en/554.pdf
AbbasSahamiDepartment of Biology, Faculty of Islamic Azad University, Jahrom Branch, Jahrom, IranAbbasSahami1225
RezaAlibakhshiNano Drug Delivery Research Centre, Kermanshah University of Medical Sciences, Kermanshah, IranRezaAlibakhshiralibakhshi@yahoo.com1231
KeyghobadGhadiriNosocomial Infectious Disease Research Center, Kermanshah University of Medical Sciences, Kermanshah, IranKeyghobadGhadiri1226
HamidSadeghiDepartment of Biology, Faculty of Islamic Azad University, Jahrom Branch, Jahrom, IranHamidSadeghi1227
en
24696796
Laparoscopic Approach to a Large Ovarian Fibroma: A Case Report
Background: Ovarian fibroma is the most common benign solid tumor of the ovary, which is often difficult to diagnose preoperatively. The choice treatment for ovarian fibroma is surgical removal, but discussions for the operative approach, laparoscopic or open, in the literature seem to be scant.
Case Presentation: We presented a unique clinical experience of laparoscopic approach to a case of 15 cm unilateral ovarian fibroma in a 24 year old patient, with a successful and complete resection of the tumor beside ovarian tissue preservation in December 2012 in Rasoul-e-Akram hospital, Tehran, Iran.
Conclusion: In conclusion, we should not forget the role of laparoscopy as a diagnostic procedure even in suspicious cases of ovarian fibroma with solid tumor, ascites, and pleural effusion.
Benign ovarian neoplasm, Laparoscopy, Ovarian fibroma, Thecoma
57
61
https://www.jri.ir/article/555
https://www.jri.ir/documents/fullpaper/en/555.pdf
ZahraNajmiMinimally Invasive Surgery Research Center, Iran University of Medical Sciences, Tehran, IranZahraNajmi1228
AbolfazlMehdizadehkashiEndometriosis Research Center, Iran University of Medical Sciences, Tehran, IranAbolfazlMehdizadehkashiamehdizadehkashi@yahoo.com1182
MaryamKadivarDepartment of Pathology, Rasoul-e-Akram Hospital, Iran University of Medical Sciences, Tehran, IranMaryamKadivar1229
ZeinabTamannaieMinimally Invasive Surgery Research Center, Iran University of Medical Sciences, Tehran, IranZeinabTamannaie1230
ShahlaChaichianMinimally Invasive Techniques Research Center of Tehran Medical Sciences Branch, Islamic Azad University, Tehran, IranShahlaChaichian1185