en
1726-7536
1735-8507
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gregorian
2019
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31897388
Evaluation of the Prevalence of Exons 1 And 10 Polymorphisms of LHCGR Gene and Its Relationship with IVF Success
<p>Background: Luteinizing hormone receptor gene shows four nonsynonymous polymorphisms within the exons. Three of these polymorphisms,<em> i.e.</em> rs4539842 (an insertion of 6bp CTGCAG at nucleotide position 54), rs12470652 (c.827A>G/p.Asn291Ser), and rs2293275 (c.935G>A/p.Ser312Asn) have been studied more frequently. Beside other hormones, LH and FSH have an important role in production of competent oocyte and female fertility. Therefore, the objective of the current study was to investigate the prevalence of exons 1(rs4539842) and 10(rs12470652, rs2293275) polymorphisms of the <em>LHCGR</em> gene and its relationship with successful IVF in Iranian infertile women. <br />
Methods: SNPs in exons 1 and 10 were analyzed in 100 women of two equally sized groups of IVF failure and IVF success women using genomic DNA. For polymorphisms in exon 10, PCR and direct sequencing were used and for the polymorphism in exon 1, RFLP technique was used. The RFLP technique is confirmed by sequencing. <br />
Results: Our results showed significant difference in allelic frequency of SNP rs2293275 among IVF successful and IVF failure groups (p=0.001). For this variation, AA genotype (A allele) was shown to have protective effect against IVF failure (p=0.03 and OR=0.04), while GG genotype (G allele) was a susceptive genotype to IVF failure (p=0.003 and OR=3.88).Allelic frequency of SNP rs4539842 also showed significant difference between the two groups (p=0.0025). For this SNP, subjects with no 6bp insertion (homozygote deletion genotype) were susceptible to be Failure in IVF (p=0.009 and OR=2.93).<br />
Conclusion: It has been revealed that two common SNPs (rs4539842 and rs2293275) in the <em>LHCGR</em> gene are associated with the outcome of IVF in Iranian infertile women. Thus, these two SNPs can be suggested to be used as predictors for IVF outcome in Iranian population.</p>
<i>In vitro</i> fertilization, Infertility, <i>LHCGR</i> gene, Polymorphism
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https://www.jri.ir/article/60061
https://www.jri.ir/documents/fullpaper/en/60061.pdf
MalihehJavadi-ArjmandDepartment of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran61973
EliaDamavandiDepartment of Photo Healing and Regeneration, Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran61974
HamidChoobinehSchool of Allied Medicine, Tehran University of Medical Sciences, Tehran, Iran61975
FereshtehSarafrazi-EsfandabadiSpecialized Medical Genetic Center of Academic Center for Education, Culture and Research (ACECR), Tehran University of Medical Sciences Branch, Tehran, Iran61976
MajidKabuliDepartment of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran61977
AtoosaMahdaviDepartment of Obstetrics and Gynecology, Dr. Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran61978
MohsenGhadamiCardiac Primary Prevention Research Center, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iranmghadami@tums.ac.ir61979