J Reprod Infert arij001 Journal of Reproduction & Infertility 2228-5482 2251-676X Avicenna Research Institute jri10020 Metabolic Fingerprinting of Seminal Plasma from Non-obstructive Azoospermia Patients: Positive Versus Negative Sperm Retrieval GilanyKambizReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, IranMetabolomics and Genomics Research Center, Endocrinology and Metabolism Molecular Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, IranJafarzadehNaserDepartment of Medical Physics, Tarbiat Modares University, Tehran, IranMani-VarnosfaderaniAhmadChemometrics and Chemoinformatics Laboratory, Department of Chemistry, Faculty of Sciences, Tarbiat Modares University, Tehran, IranMinai-TehraniArashNanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, IranSadeghiMohammad RezaReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, IranDarbandiMahsaReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, IranDarbandiSaraReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, IranAminiMehdiReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, IranArjmandBabakCell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, IranMetabolomics and Genomics Research Center, Endocrinology and Metabolism Molecular Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, IranPahlevanzadehZhamakReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran 19 2 109 115 22 8 2017 2 1 2018

<p>Background: Non-obstructive azoospermia (NOA) occurs in approximately 10% of infertile men. Retrieval of the spermatozoa from the testicle of NOA patients is an invasive approach. Seminal plasma is an excellent source for exploring to find the biomarkers for presence of spermatozoa in testicular tissue. The present discovery phase study aimed to use metabolic fingerprinting to detect spermatogenesis from seminal plasma in NOA patients as a non-invasive method.<br /> Methods: In this study, 20 men with NOA were identified based on histological analysis who had their first testicular biopsy in 2015 at Avicenna Fertility Center, Tehran, Iran. They were divided into two groups, a positive testicular sperm extraction (TESE(+)) and a negative testicular sperm extraction (TESE(-)). Seminal plasma of NOA patients was collected before they underwent testicular sperm extraction (TESE) operation. The metabolomic fingerprinting was evaluated by Raman spectrometer. Principal component analysis (PCA) and an unsupervised statistical method, was used to detect outliers and find the structure of the data. The PCA was analyzed by MATLAB software.<br /> Results: Metabolic fingerprinting of seminal plasma from NOA showed that TESE(+) versus TESE(-) patients were classified by PCA. Furthermore, a possible subdivision of TESE(-) group was observed. Additionally, TESE(-) patients were in extreme oxidative imbalance compared to TESE(+) patients.<br /> Conclusion: Metabolic fingerprinting of seminal plasma can be considered as a breakthrough, an easy and cheap method for prediction presence of spermatogenesis in NOA.</p>