J Reprod Infert arij001 Journal of Reproduction & Infertility 2228-5482 2251-676X Avicenna Research Institute jri60050 Association of Sperm Aneuploidy Frequency and DNA Fragmentation Index in Infertile Men ArumugamMeenakshiKSHEMA Centre for Genetic Services, K. S. Hegde Medical Academy, Nitte University, Mangalore, Karnataka, IndiaShettyDeyyanthodyKSHEMA Centre for Genetic Services, K. S. Hegde Medical Academy, Nitte University, Mangalore, Karnataka, IndiaKadandaleJayaramaKSHEMA Centre for Genetic Services, K. S. Hegde Medical Academy, Nitte University, Mangalore, Karnataka, IndiaNaliluSuchethaKSHEMA Centre for Genetic Services, K. S. Hegde Medical Academy, Nitte University, Mangalore, Karnataka, India 20 3 121 127 7 1 2019 16 3 2019

<p>Background: For improving the evaluation of male infertility, many parameters were studied and reported in earlier literature. The aim of this study was to estimate the frequency of sperm aneuploidy and DNA fragmentation in infertile men and to assess the correlation between sperm aneuploidy and DNA fragmentation.<br /> Methods: In this study 100 infertile men were included, cases with azoospermia were 68%, oligospermia 18%, severe oligospermia 6%, and oligoasthenoteratospermia (OAT) 8%. Ten normozoospermic men who had two normal children were included as a control. The sperm aneuploidy test by Fluorescence <em>In Situ</em> Hybridization (FISH) and sperm DNA fragmentation index by TdT (Terminal deoxynucleotidyl transferase)-mediated dUTP nick end labelling (TUNEL) were carried out. To determine the aneuploidy status and DNA fragmentation index, frequency was used. The correlation between sperm aneuploidy and sperm DNA fragmentation along with age was assessed by using Spearman&#39;s correlation coefficient. The p&lt;0.05 was considered significant.<br /> Results: The age of 100 subjects ranged between 22-48 years (35.5&plusmn;5.1). Sperm aneuploidy frequency and DNA fragmentation rate were found to be higher in infertile men compared to control men (n=10). There was a significant relationship between age and sex chromosomal aneuploidy (p&lt;0.05) and significant difference between sperm aneuploidy and damaged DNA (p&lt;0.05).<br /> Conclusion: FISH and TUNEL assay results showed increased sperm aneuploidy frequency, and DNA fragmentation index in infertile men compared with the fertile men. There is significant relationship observed between sperm aneuploidy and DNA fragmentation. These two parameters are important and they must be investigated for clinical practice.</p>