TY - JOUR T1 - Evaluation of the Utility of Seminal Plasma Resistin and Leptin in Predicting Successful Surgical Sperm Retrieval in Men with Non-Obstructive Azoospermia T2 - Journal of Reproduction & Infertility JT - Journal of Reproduction & Infertility SP - 108 EP - 117 VL - 24 IS - 2 PY - 2023 SN - 2251-676X L1 - https://www.jri.ir/documents/fullpaper/en/140185.pdf L2 - https://www.jri.ir/article/140185 UR - https://www.jri.ir/en/currentissue.aspx?id=95 PB - Avicenna Research Institute AU - Amer, Medhat AU - Zohdy, Wael AU - GamalEl Din, Sameh Fayek AU - Moawad, Hanan AU - Hasan El Saedy, Dina AU - Zaki Gamal, Eman AU - Ragab, Ahmed AB -

Background: The purpose of the current study was evaluation of the utility of seminal plasma (SP) resistin and leptin in predicting successful surgical sperm retrieval (SSR) in men with non-obstructive azoospermia (NOA).
Methods: This prospective comparative study was conducted in the andrology clinic of a specialized fertility center. In total, 53 NOA men as candidates for either first time micro‐testicular sperm extraction (micro-TESE) or repeat micro-TESE and 28 normozoospermic controls were included. ELISA was used for measurement of SP resistin and leptin levels in all participants. Significance level was defined as p<0.05.
Results: The current study demonstrated a significant positive correlation between estradiol (E2) level in serum and SP resistin (r=0.342, p=0.025). Also, there was a highly significant positive correlation between SP leptin and SP resistin (r=0.568, p=0.001). Interestingly, SP leptin was the only variable that demonstrated a significant correlation with eventful micro-TESE outcome in men who underwent micro-TESE for the first time. Finally, ROC curve showed that SP leptin level of 4.05 ng/ml predicted successful SSR in men who underwent micro-TESE for the first time with a sensitivity of 73.3% and a specificity of 75% as 11 out of 27 (41%) cases showed eventful micro-TESE at or above this cut-off level [AUC of 0.747, 95% CI, lower bound of 0.555, and upper bound of 0.939, p=0.030].
Conclusion: SP leptin can be used as a non-invasive biomarker to predict successful SSR in NOA cases undergoing first time micro-TESE, while SP resistin failed to play the same role.

CY - Tehran, Iran LA - English