The Study of Association Between Polymorphism of TNF-α Gene’s Promoter Region and Recurrent Pregnancy Loss

en 30746336 The Study of Association Between Polymorphism of TNF-α Gene’s Promoter Region and Recurrent Pregnancy Loss Background: According to the literature review, polymorphisms of tumor necrosis factor alpha’s (TNF-α) promoter region are probably the genetic risk factors of recurrent pregnancy loss. This study has investigated five single nucleotide polymorphisms in the TNF-α gene’s promoter region to evaluate their relationship with recurrent pregnancy loss disorder. Methods: Blood samples were taken from 65 women with recurrent pregnancy loss (Case group) and 65 healthy women with a history of successful pregnancy (Control group). Polymerase chain reaction with high resolution melting (PCR-HRM) analysis was done to determine the promoter region of -308G/A, -850T/C, -238G/A, -1031T/C and -863A/C TNF-α polymorphisms. The data were assessed using logistic regression models. P values less than 0.05 were considered statistically significant. Results: Significant associations were found between recurrent pregnancy loss and -863C/A (p=0.000), -308G/A (p=0.045), and -238G/A (p=0.034) polymorphisms. TNF-α polymorphisms of -863C and -238G may be susceptible factors of recurrent pregnancy loss cases. The -308G polymorphism has an important role in maintaining pregnancy. Conclusion: The -863C/A and -238G/A TNF-α polymorphisms are possible genetic risk factors of recurrent pregnancy loss and might be its predictive markers. Polymorphism, Recurrent Pregnancy Loss, Tumor necrosis factor alpha 211 219 https://www.jri.ir/article/30033 https://www.jri.ir/documents/fullpaper/en/30033.pdf RoshanakAboutorabiDepartment of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iranروشنكابوترابي229EhsanBehzadiSchool of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran31854Mohammad JavadSadeghSchool of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran31855Seyed PooriyaFatehiSchool of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran31856SorooshSemsarzadehSchool of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran31857YasamanZarrinSchool of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran31858MohammadKazemiDepartment of Genetics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran31859LalehRafieeApplied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran31860Fatemeh SadatMostafaviDepartment of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iranfs.mostafavi@gmail.com31861