en 30746337 <p>Background: The study was aimed to investigate the association of VEGFA gene polymorphic variants<em> -2578C&gt;A</em> (rs699947) and <em>-634G&gt;C</em> (rs2010963) and <em>TGFB1 </em>gene <em>915G&gt;C</em> (rs1800471) and gene expression level with miscarriage in the first trimester.<br /> Methods: 288 women with different courses of pregnancy and 61 chorionic tissue samples were involved in case-control study. Allele-specific polymerase chain reaction in real time was used for genotyping. Next, gene-gene interactions were analyzed using the multifactor dimensionality reduction method. <em>VEGFA</em> and <em>TGFВ1</em> genes expression levels were determined by RT-PCR.<br /> Results: It was found that SNP rs699947 was associated with the miscarriage risk change (p=0.05). The<em> CC</em> genotype was associated with reduced risk of abortion in the first trimester, and the <em>CA</em> genotype&nbsp; with increased risk. Genotypes <em>VEGFA -2578CС/VEGFА -634CG, VEGFA -2578AА/VEGFА -634CG</em>, and <em>VEGFA -2578CС/VEGFА -634CG/TGFВ1 936CC</em> were associated with lowered risk of miscarriage in the first trimester. mRNA level of <em>TGFВ1</em> was significantly higher in decidual tissue compared to chorionic tissue in normally progressing pregnancy (p=0.003). <em>VEGFA</em> gene expression level was directly correlated with the TGFВ1 mRNA level (R=0.60; р=0.038). In pregnancy loss, an inverse relationship was observed (R=-0.76; р=0.028).<br /> Conclusion: The SNP rs699947 is associated with pregnancy loss in the first trimester. The MDR analysis data showed the significant relationship between <em>VEGFA </em>and <em>TGFB1</em> genes in two-locus and three-locus models. A change in the ratio of the concentrations of growth factors can disrupt the processes of cell division, apoptosis and angiogenesis processes.</p> Chorionic tissue, Decidua, Gene expression, Gene polymorphic variants, Growth factors, Miscarriage 219 229 https://www.jri.ir/article/30035 https://www.jri.ir/documents/fullpaper/en/30035.pdf Tatiana MarakhovskayaDepartment of Genetics, Southern Federal University, Rostov-on-Don, Russiatmarakhovskaya@mail.ru31868ElenaButenkoDepartment of Genetics, Southern Federal University, Rostov-on-Don, Russia31869KonstantinKovalenkoDepartment of Genetics, Southern Federal University, Rostov-on-Don, Russia31870ElenaMashkinaDepartment of Genetics, Southern Federal University, Rostov-on-Don, Russia31871