en 24163795 Sperm is produced by the testis and mature in the epididymis. For having a successful conception, the fertilizing sperm should have functional competent membranes, intact acrosome, functional mitochondria and an intact haploid genome. The effects of genetic and environmental factors result in sperm vulnerability to damage in the process of spermatogenesis and maturation. In recent years, the feasibility of detecting sperm damage is enhanced through the advances in technologies like fluoscerent staining techniques assisted with fluorescence microscope, flow cytometry and computer analysis systems. Fluoscerent staining techniques involve the use of fluorescent dyes, either directly or indirectly for binding them with some ingredients of sperm and evaluating the damage of the structure or function of the sperm, i.e. membrane, acrosome, mitochondria, chromosome or DNA. Acrosome, Chromatin, DNA, Fluorescent staining, Motichondrial, Motility, Sperm function assay 120 126 https://www.jri.ir/article/533 https://www.jri.ir/documents/fullpaper/en/533.pdf Omar IbrahimFarahFamily Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaOmar IbrahimFarah1145LiCuilingFamily Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaLiCuiling1146WangJiaojiaoFamily Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaWangJiaojiao1147ZhangHuipingFamily Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaZhangHuipingzhpmed@yahoo.com.cn1148