en 31897391 <p>Background: Pre-eclampsia (PE) is the most common pregnancy complication affecting 2-8% of all pregnancies. PE could lead to maternal and prenatal morbidity. Imbalanced cytokine network and altered levels of several inflammatory and anti-inflammatory cytokines have been reported in PE. Because of scare information regarding the roles of IL-17 and IL-35 in PE, the current study aimed to investigate the serum level of these cytokines in a group of Iranian women suffering from PE.<br /> Methods: Serum samples were collected from 100 pre-eclamptic and 100 healthy pregnant women. Patients and controls were matched for age, ethnicity and body mass index. The level of IL-35 and IL-17 were evaluated by ELISA technique. T test and one-way ANOVA with Tukey Post-Hoc test were used for analysis and p&lt;0.05 were assumed significant.&nbsp;<br /> Results: The serum level of IL-35 was increased in pre-eclamptic subjects as compared with healthy pregnant women (p&lt;0.001). There was no significant difference in the serum level of IL-17 between pre-eclamptic and healthy pregnant women (p=0.73). Moreover, the results of the present study also showed that the pregnant women with severe pre-eclampsia had higher level of IL-35 in their sera when compared to those with mild form of the disease (p&lt;0.001). In addition, the serum level of IL-35 was significantly elevated in women with higher proteinuria (p&lt;0.001).<br /> Conclusion: Based on the our results, it seems that elevated levels of IL-35 in sera of pre-eclamptic women might work as a marker to evaluate the severity of the pre-eclampsia.</p> Interleukin-17, Interleukin-35, Pre-eclampsia, Pregnancy, Proteinuria 237 244 https://www.jri.ir/article/60057 /documents/fullpaper/en/60057.pdf AtefehBatebiInfertility Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran61951BahiaNamavar-JahromiInfertility Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Irannamavarb@sums.ac.ir, gharesifb@sums.ac.ir61952MohammadAli-HassanzadehDepartment of Immunology, School of Medicine, Jiroft University of Medical Sciences, Shiraz, Iran61953MoslemAhmadiDepartment of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran61954MahsaSadat HosseiniDepartment of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran61955BehrouzGharesi-FardDepartment of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran11797