The Anti-Inflammatory Effect of Octyl Gallate Through Inhibition of Nuclear Factor-κB (NF-κB) Pathway in Rat Endometriosis Model

en 32685413 The Anti-Inflammatory Effect of Octyl Gallate Through Inhibition of Nuclear Factor-κB (NF-κB) Pathway in Rat Endometriosis Model Background: Endometriosis is a chronic inflammatory condition associated with an increased risk of epithelial ovarian cancer. Our previous studies found that the anti-inflammatory effect of octyl gallate in endometriosis cell culture was more effective than gallic acid and heptyl gallate. This study aimed to analyze the anti-inflammatory effect of octyl gallate in rat endometriosis model. Methods: Thirty female Wistar rats were randomly divided into three groups. Group I was the sham-operated group, group II was the surgically-induced group, whereas group III was the surgically-induced group and each rat was administered with 20 mg of octyl gallate dissolved in 1 ml Na-CMC via oral gavage once a day for 30 days. When all rats were euthanized, the endometrial tissue from group I and last two groups were collected for further analysis. TNF-α levels were measured using Luminex, while non-phosphorylated NF-κB and COX-2 levels were analyzed using ELISA.  Results: The average of non-phosphorylated NF-κB levels in group III (4.970±0.971 pg/mgP) was significantly higher than group II (3.97±0.656 pg/mgP). Moreover, the proportion of rats with the high level of non-phosphorylated NF-κB in group III was 45.6% higher than group II (p<0.05). The proportion of rats with the high level of COX-2 in group III was 22.3% lower than group II (p<0.05). However, there was no significant difference in TNF-α levels between all groups.  Conclusion: The anti-inflammatory effect of octyl gallate may has effects in NF-κB activation and reduction of COX-2 levels in rat endometriosis model. Chronic inflammation, COX-2, Endometriosis, NF-κB, Octyl gallate 169 176 https://www.jri.ir/article/80077 /documents/fullpaper/en/80077.pdf ArleniBustami Integrated Laboratory of Medical Faculty, Universitas Indonesia, Jakarta, Indonesiaarleni.ab@gmail.com82031WahyuLestariMaster Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia82032CiciliaHayuningrumDepartement of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia82033HeriWibowoDepartement of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia82034PuspitaWuyungDepartement of Pathology Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia82035R. MuharamNatadisastraDepartement of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia82036