en
1726-7536
1735-8507
69
2139
288
gregorian
2011
4
23
12
1
online
1
fulltext
en
23926492
Epigenetic Alterations and their Impact on Assisted Reproduction Technology Outcomes
01
2
https://www.jri.ir/article/569
https://www.jri.ir/documents/fullpaper/en/569.pdf
Mohammad RezaSadeghiEditor-in-chief, Tehran, Iran محمدرضاصادقی77
en
23926493
Effects of Kamdhenu Ark and Active Immunization by Gonadotropin Releasing Hormone Conjugate (GnRH-BSA) on Gonadosomatic Indices (GSI) and Sperm Parameters in Male Mus musculus
Background: Active immunization against GnRH decreases the secretion of gonadotropins and causes cessation of gonadal function, thereby, inducing infertility. Based on the immunoenhancing activity of Kamdhenu ark (distilled cow urine), this study was performed to evaluate its effects on the gonadosomatic indices (GSI) and sperm parameters in male mice receiving a GnRH contraceptive vaccine.
Methods: Sixty adult male mice of Parke’s strain were divided into three groups of twenty. Group I served as the controls, while group II was immunized by GnRH-BSA conjugate (50/0.2/35 µg/ml/g BW) by four intraperitoneal injections at different intervals on days 1, 30, 60 and 90. However, group III was supplemented daily by oral Kamdhenu ark (100 ppm) along with GnRH-BSA immunizations. The animals were sacrificed after 30, 60, 90 and 120 days and their testis and epididymis were dissected out weighed and semen analysis was performed.
Results: GSI values, sperm motility, sperm count and sperm morphology in male Mus musculus were decreased significantly in all the experimental groups as compared to the control group (p<0.01). Kamdhenu ark significantly enhanced the effect of GnRH vaccine on the aforesaid parameters especially in 90 and 120 days treated groups (p<0.05).
Conclusion: The changes witnessed in sperm parameters suggested that the GnRH-BSA immunization suppressed the activities of gonadotropins and testosterone directly through hypothalamo-hypophysial-gonadal axis and indirectly by acting on the testes which may modulate the sperm morphology, sperm count and motility. However, Kamdhenu ark seems to have enhanced these effects because of its immunemodulatory properties too.
GnRH-BSA، ایمن سازی، واکسیناسیون، اندکسهای گونادوسوماتیک، ادرار گاو ماده، پارامترهای اسپرم
GnRH-BSA, Gonadosomatic indices (GSI), Immunization, Kamdhenu ark, Mus musculus, Sperm Parameters
03
8
https://www.jri.ir/article/460
https://www.jri.ir/documents/fullpaper/en/460.pdf
JavidAhmad GanaieEndocrinology Laboratory, Department of Biosciences, Barkatullah University, Bhopal, Indiaجاويداحمد گانعي921
VarshaGautamEndocrinology Laboratory, Department of Biosciences, Barkatullah University, Bhopal, Indiaوارشاگواتام922
VinoyKumar ShrivastavaEndocrinology Laboratory, Department of Biosciences, Barkatullah University, Bhopal, Indiaوينويكومار شريواستاواvinoyks2001@yahoo.com923
en
23926494
PCR-Based Evaluation of Tuberculous Endometritis in Infertile Women of North India
Background: Tuberculous endometritis is often a diagnostic dilemma for gynecologists in the evaluation of infertility. This study evaluated and compared different diagnostic methods in tuberculous endometritis.
Methods: 100 infertile women were investigated for tuberculous endometritis. The endometrial samples obtained by curettage were evaluated by Ziehl-Neelsen (ZN) staining of the smears for acid-fast bacilli (AFB), the samples were also cultured on Lowenstein-Jensen medium, and histopathological examination and nested PCR targeting ‘hupB’ gene (Rv 2986c in M. tuberculosis and Mb3010c in M. bovis) which can differentiate between M. tuberculosis and M. bovis were performed. Antibodies against 38-kDa and 16-kDa mycobacterial antigens were detected in serum using ELISA. Statistical analysis was done by online GraphPad Prism software, version 5.0. McNemar’s test was applied and Kappa agreement coefficient was calculated for agreement between various methods. A p-value < 0.05 was considered significant.
Results: Among the 100 evaluated endometrial samples, one was AFB smear positive, none was positive by culture, four were positive by histopathology and 13 were positive by PCR. Of the 13 PCR-positive cases, 38.4% were positive for M. tuberculosis, 23.07% for M. bovis, and 38.4% showed co-infection with both species. 40% of the patients had raised IgG against M. tuberculosis 38-kDa antigen. McNemar’s test was applied to PCR and the conventional methods of TB diagnosis (AFB, Culture and histopathology) and the p-value was < 0.001 (highly significant) for PCR. Detection by PCR showed a fair agreement with detection by Mantoux test and ELISA.
Conclusion: In paucibacillary endometrial tuberculosis, the positive detection rate was found to be significantly higher for PCR compared to other methods. The ‘in-house’ nested PCR assay targeting the hupB gene and used in this study, can serve as a rapid diagnostic aid for tubercular endometritis. It can also differentiate between members of the Mycobacterium tuberculosis complex, namely M. tuberculosis and M. bovis.
ناباروری، مایکوباکتریوم توبرکولوز، توبرکولوزسلی، PCR
Infertility, M. bovis, M. tuberculosis, PCR, Tuberculous endometritis
09
15
https://www.jri.ir/article/461
https://www.jri.ir/documents/fullpaper/en/461.pdf
MonikaD. KohliDepartment of Biochemistry, Lady Hardinge Medical College and S.S.K. Hospital, New Delhi, Indiaمونيكاكوهلي924
BimotaNambamDepartment of Biochemistry, Lady Hardinge Medical College and S.S.K. Hospital, New Delhi, Indiaبيموتانام بام925
SagarS. TrivediDepartment of Obstetrics and Gynecology, Lady Hardinge Medical College and S.S.K. Hospital, New Delhi, Indiaساگرتراي ودي926
BankeL. SherwalDepartment of Microbiology, Lady Hardinge Medical College and S.S. K. Hospital, New Delhi, Indiaبنكشروال927
SarikaAroraDepartment of Biochemistry, Lady Hardinge Medical College and S.S.K. Hospital, New Delhi, Indiaساريكاآرورا861
AnjuJainDepartment of Biochemistry, Lady Hardinge Medical College and S.S.K. Hospital, New Delhi, Indiaآنجوجينdranjujain@rediffmail.com928
en
23926495
Effects of Maternal Caffeine Consumption on Ovarian Follicle Development in Wistar Rats Offspring
Background: In recent years concerns have been raised about human reproductive disorders, specially the effects of environmental factors on human fertility and pregnancy outcome. Therefore, the present study was designed to assess the effects of maternal caffeine consumption on ovarian follicles development in rat offspring.
Methods: 60 pregnant female rats were randomly divided into a control and two experimental groups. The rats in the two experimental groups received caffeine via drinking water during gestation (26 and 45 mg/kg) and lactation (25 and 35 mg/kg). The ovaries of the offspring were removed at 7, 14, 28, 60, 90 and 120 days after birth, and fixed in Bouin’s solution. By preparing serial tissue sections, structural changes in ovarian follicles and corpora lutea were studied during postnatal development.
Results: The weight of ovaries decreased significantly (p<0.05) in the high dose caffeine-treated group at all stages of postnatal development. Significant (p<0.05) decreases were seen in the number of primordial follicles from day 7 to 120 after birth in the high dose caffeine-treated group. Moreover, the number of primary and secondary follicles decreased significantly on days 7, 14 and 28 as did the number of antral follicles on days 14 and 28 after birth (p<0.05) in the high dose caffeine-treated group. The diameter of secondary and antral follicles decreased significantly (p<0.05) in high dose caffeine-treated group on the early days of postnatal development. No statistically significant differences were seen in the number of corpora lutea between the groups.
Conclusion: The present study shows that caffeine consumption during gestation and lactation affects the early stages of ovarian follicle development and reduces reproductive efficiency in the offspring of Wistar rats.
باروری، تکامل پس از تولد، فولیکول های تخمدانی، کافئین
Caffeine, Fertility, Ovarian follicles, Postnatal development
15
23
https://www.jri.ir/article/462
https://www.jri.ir/documents/fullpaper/en/462.pdf
MehranDorostghoalDepartment of Biology, Faculty of Sciences, Shahid Chamran University of Ahwaz, Ahvaz, Iranمهراندرست قولmdorostghoal@yahoo.com916
MahmoodKhaksari MahabadiDepartmant of Anatomy, Faculty of Veterinary, Shahid Chamran University of Ahwaz, Ahvaz, Iranمحمودخاكساري مهابادي918
SaharAdhamDepartment of Biology, Faculty of Sciences, Shahid Chamran University of Ahwaz, Ahvaz, Iranسحرادهم917
en
23926496
Reproductive Health Rights of Women in the Rural Areas of Meherpur District in Bangladesh
Background: This study evaluated the reproductive health rights, women empowerment and gender equity in a rural area of Bangladesh.
Methods: Three hundred married women of reproductive age (15-49 years) in Meherpur District, Bangladesh were interviewed using a structured questionnaire and purposing sampling techniques. The logistic regression analysis was used to determine the dominating factors affecting reproductive health rights. To fulfill the objectives of the study the two main factors, age at marriage and family planning acceptance of the respondents, were regarded as the determinants.
Results: The study results revealed that almost all the respondents were housewives (82.3%), one-third (31.0%) did not avail any modern facility, and their yearly income was very low. Moreover, about half of the women (52.7%) were very young ( 30 years), most of them (79.0%) had married early (<18 years) and about half of them (53.3%) had taken contraceptives based on their husbands’ choice. Finally, multivariate analysis identified the relationship between the profession of the respondents, yearly income, number of family members, and the availability of modern facilities with age at marriage (Model 1). The study also identified the relationship between the age of respondents, education, occupation, yearly income, and the total number of family members with family planning acceptance (Model 2).
Conclusion: Regarding the results of this study, women’s reproductive health rights, marriage after the age of 18 and family planning acceptance among couples needs to be enhanced in Mehrpur District in Bangladesh.
توانمند سازی، جنسیت، حقوق باروری، زنان، بهداشت باروری
Empowerment, Gender, Reproductive rights, Women
23
33
https://www.jri.ir/article/457
https://www.jri.ir/documents/fullpaper/en/457.pdf
Md KamalHossainDepartment of Population Science and Human Resource Development, University of Rajshahi, Rajshahi, Bangladeshكمالحسين محمد903
Md Nazrul IslamMondalDepartment of Population Science and Human Resource Development, University of Rajshahi, Rajshahi, Bangladeshنظرالاسلامموندال محمدnazrul_ru@yahoo.com904
Mst NaznizAkterDepartment of Population Science and Human Resource Development, University of Rajshahi, Rajshahi, Bangladeshنازنيناختر سمت905
en
23926497
Iranian Oncologists’ Attitude towards Fertility Preservation in a Sample Group
Background: Recent advances in cancer treatment have resulted in an increased number of cancer survivors. Fertility might be impaired by cancer itself or through gonadal damage as a consequence of radio-chemotherapy. Damages to reproductive organs are seen in minimally upto 30% of cancer patients. The aim of the present study was evaluate the attitude of Iranian oncologists toward fertility preservation.
Methods: Thirty oncologists filled an author-designed questionnaire which included five multiple choice questions at Mahak hospital, Tehran in 2009. The data were collected and analyzed by SPSS 11.5 software.
Results: More than half of the oncologists believed that radio-chemotherapy has destructive effects on reproductive organs. This study showed that as high as 67% of the respondants believed fertility preservation should be offered to all patients at risk, but 40% of them insisted that the patients should bring up the topic themselves. Only 46% of the oncologist knew about fertility preservation techniques. The greatest barriers for accepting fertility preservation by the parents of children undergoing cancer treatment were: lack of information (41%), hopelessness from treatment prognosis (33%), fear of delay in starting the treatment (15%) and the priority of child survival (11%).
Conclusion: The specific challenges of counseling are time pressure between diagnosis and start of treatment, little knowledge about fertility preservation both by treating oncologists and parents; therefore a team consisting of oncologists, infertility specialists and social workers is recommended in these settings.
انکولوژیست، نگرش، سرطان، حفظ باروری، رادیو تراپی و شیمی درمانی
Attitude, Cancer, Fertility preservation, Oncologist, Radio-chemotherapy
33
37
https://www.jri.ir/article/463
https://www.jri.ir/documents/fullpaper/en/463.pdf
BehzadGhorbaniReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iranبهزادقربانی26
PouranMadahiMahak Hospital, Tehran, Iranپورانمداحی919
ElhamShiraziDepartment of Psychiatry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iranالهامشيرازي818
HoomanSadri-ArdekaniReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iranهومن صدرياردكاني291
KooroshKamaliReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, IranK.Kamali@avicenna.ac.ir1427
en
23926498
Post-Delivery Cardiomyopathy in a Patient Admitted to Critical Care Unit; A Rare Case Report
Background: Peripartum cardiomyopathy (PPCM) is an uncommon disease that affects women in the last month of pregnancy or within the first five months postpartum, occurring in about 1 in 3500 live births. The disease bears potentially devastating effects both on mother and the fetus if not treated early in its course.
Case Presentation: The case was a 34-year old woman with a triple pregnancy who presented to the ward immediately after cesarean section with signs of dyspnea, cyanosis and pulmonary edema. She was diagnosed with PPCM upon echocardiography. The patient improved remarkably despite the PPCM’s devastating complications. This case report aims to describe a female patient who developed PPCM after a triple delivery.
Conclusion: Regarding the high risks of developing PPCM in subsequent pregnancies and avoiding multiparty, especially in older age, a reliable contraception in childbearing women would be helpful. The best prevention of PPCM is to avoid subsequent pregnancies.
جراحی سزارین، اکوکاردیوگرافی، فشار خون بارداری، میوکاردیت، کاردیومیوپاتی دوران نفاس، مسمومیت بارداری، فنومن ترومبوامبولی
Caesarian section, Echocardiography, Gestational hypertension, Myocarditis, Peripartum cardiomyopathy, Preeclampsia, Thrombophilic phenomena
37
42
https://www.jri.ir/article/459
https://www.jri.ir/documents/fullpaper/en/459.pdf
MohammadrezaSafaviAnesthesiology and Critical Care Research Center, Isfahan University of Medical Sciences, Isfahan, Iranمحمد رضاصفوي909
AzimHonarmandAnesthesiology and Critical Care Research Center, Isfahan University of Medical Sciences, Isfahan, Iranعظيمهنرمندhonarmand@med.mui.ac.ir910
ParvizKashefiAnesthesiology and Critical Care Research Center, Isfahan University of Medical Sciences, Isfahan, Iranپرويزكاشفي911
Saied MortezaHeidariAnesthesiology and Critical Care Research Center, Isfahan University of Medical Sciences, Isfahan, Iranسعيد مرتضيحيدري912
AfshinSafaviAnesthesiology and Critical Care Research Center, Isfahan University of Medical Sciences, Isfahan, Iranافشينصفوي913
RitaHekmatDepartment of Gynecology and Obstetrics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iranريتاحكمت914
en
23926499
Publications about Sperm during the Years 1897 to 2010
<p>Sperm cells are one of the most stellar and fas-cinating cells in all species. One of the most distinctive characteristic of these cells is their movement dynamics which can be observed through a light microscope. The first paper regarding sperm cell biology that appeared on PubMed was pres-ented by Dr. W.F. Whitney (1). There, he showed the staining properties of spermatozoa and it was published in the Boston Society of Medical Sciences Journal in 1897. From that time on, around 82,998 papers have been published about sperm. The objective of the present paper was to evaluate the dynamic of sperm publication in the world using PubMed indexing database between 1897 and 2010 and the percentage of papers published on sperm per year.</p>
43
44
https://www.jri.ir/article/458
https://www.jri.ir/documents/fullpaper/en/458.pdf
MayaWalter CardonaGrupo Reproducción, Universidad de Antioquia, Medellín, Colombiaماياوالتر كاردوناwdcmaya@medicina.udea.edu.co908