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31423413
Coming Soon: Disclosing the Identity of Donors by Genealogical Tests of Donor Offspring
119
121
https://www.jri.ir/article/60053
https://www.jri.ir/documents/fullpaper/en/60053.pdf
Mohammad RezaSadeghiReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran محمدرضاصادقیsadeghi@avicenna.ac.ir77
en
31423414
Association of Sperm Aneuploidy Frequency and DNA Fragmentation Index in Infertile Men
<p>Background: For improving the evaluation of male infertility, many parameters were studied and reported in earlier literature. The aim of this study was to estimate the frequency of sperm aneuploidy and DNA fragmentation in infertile men and to assess the correlation between sperm aneuploidy and DNA fragmentation.<br />
Methods: In this study 100 infertile men were included, cases with azoospermia were 68%, oligospermia 18%, severe oligospermia 6%, and oligoasthenoteratospermia (OAT) 8%. Ten normozoospermic men who had two normal children were included as a control. The sperm aneuploidy test by Fluorescence <em>In Situ</em> Hybridization (FISH) and sperm DNA fragmentation index by TdT (Terminal deoxynucleotidyl transferase)-mediated dUTP nick end labelling (TUNEL) were carried out. To determine the aneuploidy status and DNA fragmentation index, frequency was used. The correlation between sperm aneuploidy and sperm DNA fragmentation along with age was assessed by using Spearman's correlation coefficient. The p<0.05 was considered significant.<br />
Results: The age of 100 subjects ranged between 22-48 years (35.5±5.1). Sperm aneuploidy frequency and DNA fragmentation rate were found to be higher in infertile men compared to control men (n=10). There was a significant relationship between age and sex chromosomal aneuploidy (p<0.05) and significant difference between sperm aneuploidy and damaged DNA (p<0.05).<br />
Conclusion: FISH and TUNEL assay results showed increased sperm aneuploidy frequency, and DNA fragmentation index in infertile men compared with the fertile men. There is significant relationship observed between sperm aneuploidy and DNA fragmentation. These two parameters are important and they must be investigated for clinical practice.</p>
DNA fragmentation, Male infertility, Sperm aneuploidy, TUNEL assay
121
127
https://www.jri.ir/article/60050
https://www.jri.ir/documents/fullpaper/en/60050.pdf
MeenakshiArumugamKSHEMA Centre for Genetic Services, K. S. Hegde Medical Academy, Nitte University, Mangalore, Karnataka, India61919
DeyyanthodyShettyKSHEMA Centre for Genetic Services, K. S. Hegde Medical Academy, Nitte University, Mangalore, Karnataka, Indiadprashanthshetty@gmail.com61920
JayaramaKadandaleKSHEMA Centre for Genetic Services, K. S. Hegde Medical Academy, Nitte University, Mangalore, Karnataka, India61921
SuchethaNaliluKSHEMA Centre for Genetic Services, K. S. Hegde Medical Academy, Nitte University, Mangalore, Karnataka, India61922
en
31423415
The Frequency of Chromosomal Euploidy Among 3PN Embryos
<p>Background: The evaluation of embryo morphology is one of the most important parameters used to evaluate developmental timing, also providing an indication of chromosomal failure or degeneration. The first step in the evaluation of a fertilization event is determining the number and shape of the pronuclei (PN). Normally fertilized eggs possess two even PN. However, some embryos which develop from abnormally fertilized zygotes may be tri-pronuclear zygotes (3PN). <br />
Methods: Thirty embryos were collected from 12 women who underwent <em>in vitro</em> fertilization (IVF) at Dr. Cipto Mangunkusumo General Hospital in Jakarta, Indonesia. Embryos were cultured until the blastocyst stage on days 5-6. The blastomere biopsy was performed by piercing the zona pellucida with a laser under a microscope. Chromosomal numerical abnormalities were analyzed using Next Generation Sequencing (NGS).<br />
Results: Among the 30 embryos with 3PN zygotes, 33.3% had a normal chromosomal array, with 22 pairs of autosomes and 2 pairs of sex chromosomes. While the rest of sample population detected as abnormal chromosome (66.7%), with the highest percentage of abnormality was triploidy 43.3%, followed by mosaicism 13.4% and aneuploidy 10%.<br />
Conclusion: This was a preliminary study revealed not all morphologically 3PN embryos are genetically abnormal.</p>
Aneuploidy, Embryo, IVF, Mosaisicm, Pronucleus
127
132
https://www.jri.ir/article/60052
https://www.jri.ir/documents/fullpaper/en/60052.pdf
KresnaMutiaHuman Reproductive, Infertility and Family Planning Research Center, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia61927
BudiWiwekoYasmin IVF Clinic, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesiabudiwiweko@gmail.com61928
PrittaIffanolidaHuman Reproductive, Infertility and Family Planning Research Center, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia61929
RirinFebriHuman Reproductive, Infertility and Family Planning Research Center, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia61930
NaylahMunaHuman Reproductive, Infertility and Family Planning Research Center, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia61931
OkiRiayatiHuman Reproductive, Infertility and Family Planning Research Center, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia61932
ShantyJasirwanHuman Reproductive, Infertility and Family Planning Research Center, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia61933
TitaYuningsihYasmin IVF Clinic, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia61934
ElizaMansyurYasmin IVF Clinic, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia61935
AndonHestiantoroYasmin IVF Clinic, Dr. Cipto Mangunkusumo General Hospital, Jakarta, Indonesia61936
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31423416
Association Between Sex Steroids and Oxidative Status with Vitamin D Levels in Follicular Fluid of Non-obese PCOS and Healthy Women
<p>Background: Human follicular fluid (FF) is rich in hormones and antioxidants. Many components of FF differ in follicles of patients with polycystic ovary syndrome (PCOS). Regarding vitamin D effects on gene expression, 25(OH)D level of FF and its association with oxidative status and sex steroids dysregulation in PCOS group was evaluated and compared to controls of Non-obese healthy women. <br />
Methods: FF of 50 non-obese healthy women and 50 women with PCOS (18-36 years old) who were candidates for IVF/ICSI was aspirated on the oocyte retrieval day. Sex steroids and 25(OH)D levels were measured by ELISA. Reactive oxygen species (ROS) levels, total antioxidant capacity (TAC), and activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) were assessed by chemiluminescence and spectrophotometric methods. Data were analyzed by unpaired t-test or Mann-Whitney test, and Pearson correlation coefficient. The p<0.05 was considered statistically significant.<br />
Results: Estradiol, progesterone, 25(OH)D, TAC, and activities of SOD, GPx, and CAT in FF of women with PCOS were significantly lower, whilst their free and total testosterone and ROS levels were significantly higher than controls. There were significant positive correlations between FF levels of 25(OH)D with TAC, estradiol and progesterone concentrations, SOD, GPx, and CAT activities. Negative correlations were found between 25(OH)D with free and total testosterone, and ROS levels. <br />
Conclusion: Despite different hormonal and antioxidant levels in FF of normal and cystic follicles, the correlation between 25(OH)D levels with sex steroids and oxidative stress markers showed a possible role of 25(OH)D in regulating sex hormones secretion and enhancement of antioxidant defense.</p>
Oxidative stress, Polycystic ovarian syndrome, Sex steroid hormones, Vitamin D
132
143
https://www.jri.ir/article/60047
https://www.jri.ir/documents/fullpaper/en/60047.pdf
FatemehMasjediDepartment of Physiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran61903
SaraKeshtgarDepartment of Physiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Irankeshtgar@sums.ac.ir, keshtgars@yahoo.com11836
FatemehAgahDepartment of Physiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran61904
NargesKarbalaeiDepartment of Physiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran61905
en
31423417
Prediction of Responsiveness to Clomiphene Citrate in Infertile Women with PCOS
<p>Background: The purpose of the study was to evaluate the role of clinical, metabolic, hormonal and ultrasound features of women with PCOS in predicting the response to clomiphene citrate in treatment of infertility.<br />
Methods: A prospective observational study was done over a period of one year. A total of 164 women with PCOS related infertility were enrolled. They were treated with an incremental dose of clomiphene citrate starting with 50 <em>mg/day</em> to a maximum of 150 <em>mg</em> over 3 cycles. The response was recorded as either presence or absence of ovulation. Multiple logistic regression was used to analyze various clinical, metabolic, hormonal and ultrasound features in these women. Sensitivity and specificity of each of these parameters in predicting non-responsiveness (failure to ovulate with 150 <em>mg</em> clomiphene) were calculated. <br />
Results: Ferriman-Gallwey score, androstenedione levels, HDL, and cholesterol were found to be the independent predictors of non-responsiveness to clomiphene citrate. The overall best predictor of non-responsiveness to clomiphene citrate is Ferriman- Gallwey score (FG). FG score, with a cut off value of 15, had 73.9% sensitivity and 86.8% specificity in predicting non-responsiveness to clomiphene. BMI was the best anthropometric predictor of the non-responsiveness to clomiphene. Fasting insulin was the best metabolic predictor of the non-responsiveness to clomiphene. AFC was the best ovarian reserve marker as the predictor of the non-responsiveness to clomiphene (cut-off value of 11.75 with 73.9% sensitivity and 73.7% specificity). <br />
Conclusion: Ferriman-Gallwey score, androstenedione levels, and lipid profile are clinically useful parameters to predict which groups of PCOS women are unlikely to respond to clomiphene. </p>
Anti- mullerian hormone, Body Mass Index, Clomiphene, Hyperandrogenism, Polycystic ovarian syndrome
143
151
https://www.jri.ir/article/60049
https://www.jri.ir/documents/fullpaper/en/60049.pdf
GarimaSachdevaDepartment of Obstetrics and Gynaecology, Post Graduate Institute of Medical Education and Research, Chandigarh, Indiagsachdeva25@gmail.com61914
ShaliniGainderDepartment of Obstetrics and Gynaecology, Post Graduate Institute of Medical Education and Research, Chandigarh, India61915
VanitaSuriDepartment of Obstetrics and Gynaecology, Post Graduate Institute of Medical Education and Research, Chandigarh, India61916
NareshSachdevaDepartment of Endocrinology, Post Graduate Institute of Medical Education and Research, Chandigarh, India61917
AroniehChopraDepartment of Obstetrics and Gynaecology, Post Graduate Institute of Medical Education and Research, Chandigarh, Indiaآرونيهچوپرا423
en
31423418
Impact of Genetic Variants in Estrogen Receptor-β Gene in the Etiology of Uterine Leiomyomas
<p>Background: Uterine leiomyomas are steroid hormone dependent myometrial neoplasms of female genital tract which appear after menarche and regress at menopause. The present study evaluated the role of ER-β gene polymorphisms (rs3020449 C/T, rs3020450 G/A, rs1271572 G/T, rs1256049 G/Aand rs4986938 G/A) in the etiology of disease. <br />
Methods: A total of 150 clinically, ultrasonographically evaluated uterine leiomyoma patients and an equal number of individuals as controls were considered for the present study. Genotype analysis was carried out by TETRA Primer Amplification Refractory Mutation System–PCR for promoter polymorphisms and PCR- RFLP method was done for exonic polymorphisms followed by agarose gel electrophoresis. The strength of the association of ER-β gene polymorphisms between controls and patients were measured by appropriate statistical methods.<br />
Results: An increased frequency of T/T genotype and T allele of rs3020449, AA genotype and A allele of rs3020450, T/T genotype and T allele of rs1271572, AA genotype and A allele of rs1256049 and A/A genotype and A allele of rs4986938 was observed in cases when compared with controls.<br />
Conclusion: The study indicates that the ER-β gene polymorphisms may act as a major genetic regulator in the etiology of uterine leiomyomas.</p>
Cell proliferation, Estrogens, Leiomyoma, Myometrium, Neoplasms, Transcription factors, Uterus
151
161
https://www.jri.ir/article/60051
https://www.jri.ir/documents/fullpaper/en/60051.pdf
ChitrojuBharathiInstitute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, Telangana, India61923
DesamalaAnupamaDepartment of Gynaecology, Modern Government Maternity Hospital, Hyderabad, Telangana, India61924
NallariPratibhaInstitute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, Telangana, Indiaنالاريپراتيبها841
AnantapurVenkateshwariInstitute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, Telangana, Indiavenkateshwari@yahoo.com61926
en
31423419
Nutrient Patterns and Risk of Polycystic Ovary Syndrome
<p>Background: There are limited data on the role of nutrient patterns in development of polycystic ovary syndrome (PCOS). The aim of the study is to document the relationship between nutrient patterns and PCOS.<br />
Methods: In this study, 281 incident PCOS women and 472 controls were interviewed through the endocrine clinics between February 2013 and March 2015 in Tehran, Iran. Usual dietary intakes were obtained using a validated semi-quantitative food frequency questionnaire. Factor analysis was conducted on the basis of 32 nutrients. Unconditional logistic regression was performed to ascertain odds ratios. The p<0.05 was considered for significance level.<br />
Results: In principal component analysis two nutrient patterns emerged. Factor 1 had high loadings for riboflavin, niacin, pyridoxine, thiamin, magnesium, pantothenic acid, cobalamin, vitamin C, folate, vitamin D, total fiber, selenium, phosphorus, vitamin E, manganese, vitamin K, monounsaturated fatty acids, polyunsaturated fatty acids, potassium and vegetable protein. Factor 2 characterized by high loadings for carbohydrate, animal protein, fat, cholesterol, saturated fatty acid, sodium, biotin, copper, iron, fluoride, zinc, and calcium. After adjusting for potential confounders, the risk of PCOS was significantly higher in the highest tertile of factor 2 (OR: 2.38, 95% CI: 1.69-3.21). Conversely, being in the highest tertile of factor 1 was associated with a lower risk of PCOS (OR: 0.48, 95% CI: 0.21-0.82). <br />
Conclusion: Our results provide a possible new insight into the interactions between nutrient intakes and PCOS.</p>
Macronutrients, Micronutrients, Nutrient patterns, Polycystic ovarian syndrome, Principal component analysis
161
169
https://www.jri.ir/article/60046
https://www.jri.ir/documents/fullpaper/en/60046.pdf
GhazalehEslamianNational Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran61901
AzitaHekmatdoostNational Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Irana_hekmat2000@yahoo.com61902
en
31423420
Effects of Group Counseling on Stress and Gender-Role Attitudes in Infertile Women: A Clinical Trial
<p>Background: Infertility stress can have a devastating impact on the lives of couples and influence their physical and psychological health. The purpose of this study was to investigate the effects of group counseling on female stress and gender-role attitudes in infertile women.<br />
Methods: The present study is a randomized clinical trial conducted on 90 infertile women referred to Rooyesh Infertility Treatment Center in the city of Karaj, Iran. The convenience sampling method was used. Samples were divided into intervention and control groups through four-block random allocations. Accordingly, the intervention group received five-session group counselling and the control group only received routine care. Newton’s fertility problem inventory (FPI) and gender role questionnaire (GRQ) were used for collecting data before, after, and one month after the intervention. The significance level was set at 0.05.<br />
Results: The result showed a significant relationship between gender role attitude and stress in infertile women (p=0.03) and indirect association between of them (r=0.13). And also repeated measures test indicated that length of time had affected the total scores of infertility stress (p<0.001) and gender role attitude scores (p=0.001) and there was a significant difference between the two groups in infertility stress scores (p<0.001) and gender role attitude scores (p=0.001). <br />
Discussion: Group counseling can be used in stress reduction and also improved gender role attitude of infertile women.</p>
Counseling, Gender role, Infertility, Stress
169
178
https://www.jri.ir/article/30042
https://www.jri.ir/documents/fullpaper/en/30042.pdf
ZeinabEhsanStudent Research Committee, School of Medical Sciences, Alborz University of Medical Sciences, Karaj, Iran61894
MansoorehYazdkhastiNon-communicable Diseases Research Center, Alborz University of Medical Sciences, Karaj, Iran61895
MitraRahimzadehSocial Determinants of Health Research Center, Alborz University of Medical Sciences, Karaj, Iran61896
MinaAtaeeDepartment of Obstetrics and Gynecology, Clinical Research Development Center of Kamali Hospital, School of Medical Sciences, Alborz University of Medical Sciences, Karaj, Iran61897
SaraEsmaelzadeh-SaeiehNon-communicable Diseases Research Center, Alborz University of Medical Sciences, Karaj, IranEsmaelzadeh1360@gmail. com61898
en
31423421
The Social Construction of Infertility Among Iranian Infertile Women: A Qualitative Study
<p>Background: Infertility is considered an important phenomenon in couples’ life. Infertility and its treatment process influence all aspects of the individual’s life. This study aimed to explain the psycho-social process of social construction of infertility among Iranian infertile women.<br />
Methods: This was a qualitative study using a grounded theory approach. The study setting was the Vali-e-Asr Fertility Health Research Center and Avicenna Fertility clinic in Tehran. The sampling started purposefully and it was continued theoretically. The data collection was performed by using 36 semi-structured interviews, observation and field notes with 27 women who suffered from primary and secondary infertility having no living child. The method suggested by Strauss and Corbin was used for data analysis.<br />
Results: Results indicate that "Concerns over life instability" and "being judged by others" were the participants’ most important preoccupation. Attempts to stabilize life and get rid of being judged by others were key aspects of the social construction of infertility and the main strategies for resolving their preoccupation. This core concept explained the basic psychological-social process of infertility in relation to axial codes.<br />
Conclusion: The results of the study show that various interactive factors affect the social construction of infertility among infertile women who focus on the central concept of attempts to stabilize life and get rid of being judged by others. Therefore, in order to achieve this goal, infertile women should be empowered by effective coping strategies.</p>
Grounded theory, Infertile women, Infertility, Social construction
178
191
https://www.jri.ir/article/60045
https://www.jri.ir/documents/fullpaper/en/60045.pdf
Syedeh BatoolHasanpoor-AzghadyDepartment of Reproductive Health and Midwifery, Nursing Care Research Center (NCRC), School of Nursing and Midwifery, Iran University of Medical Science, Tehran, Iran61899
MasumehSimbarMidwifery and Reproductive Health Research Center (MRHRC), Department of Midwifery and Reproductive Health, School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, Iranمعصومهسیمبرmsimbar@yahoo.com, msimbar@sbmu.ac.ir779
Abu AliVedadhirDepartment of Anthropology, Faculty of Social Sciences, University of Tehran, Tehran, Iranابوعلیودادهیر30
Seyed AliAzinReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran19
LeilaAmiri-FarahaniDepartment of Reproductive Health and Midwifery, Nursing Care Research Center (NCRC), School of Nursing and Midwifery, Iran University of Medical Science, Tehran, Iran61900
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31423422
Patient with Disorders of Sex Development (DSD): A Case Report from a Tertiary Care Hospital in Thiruvananthapuram, India
<p>Background: 46 XX male syndrome, a rare case of infertility was first reported by de la Chapelle in 1964. In newborn males, the incidence rate of the syndrome varies from 1/9000 to 1/20000. Here, a case of 46 XX male syndrome is reported with clinical, biochemical and genetic changes of the patient and normal masculine features.<br />
Case Presentation: A 29 year old male with infertility registered at the Sree Avittom Thirunal Hospital of Government Medical College, Thiruvananthapuram for fertility treatment. He was diagnosed with non obstructive azoospermia in repeated semen analysis. Chromosomal analysis on peripheral blood lymphocytes has revealed 46 XX male syndrome and the result was confirmed with Fluorescent In situ Hybridization (FISH). Real time polymerase chain reaction failed to detect genes on azoospermia factor regions, AZFa, AZFb and AZFc of Y chromosome, but detected SRY gene positivity. Masculine features of patient were normal except small sized testis, ejaculatory dysfunction and azoospermia. <br />
Conclusion: Appearance of the external genitalia will be generally normal in 46 XX with SRY positive males and generally difficult to identify before puberty because there will not be any significant clinical indication. The present case report demonstrates that mere physical or clinical examination may not disclose the genetic defects. Therefore, in addition to general examination, it is essential to perform genetic analysis on men with infertility.</p>
46 XX male syndrome, Azoospermia, SRY gene, Y chromosome microdeletion
191
195
https://www.jri.ir/article/60048
https://www.jri.ir/documents/fullpaper/en/60048.pdf
PongillyathundiyilSreejithMulti Disciplinary Research Unit, Government Medical College, Thiruvananthapuram, India61906
SheilaBalakrishnanDepartment of Reproductive Medicine and Surgery, Sree Avittom Thirunal Hospital, Government Medical College, Thiruvananthapuram, India61907
VaikomSankarDepartment of Pediatrics, Sree Avittom Thirunal Hospital, Government Medical College, Thiruvananthapuram, India61908
RemyaSyamalaChild Development Centre, Government Medical College, Thiruvananthapuram, India61909
RejiMohanDepartment of Reproductive Medicine and Surgery, Sree Avittom Thirunal Hospital, Government Medical College, Thiruvananthapuram, India61910
SankarSundaramDepartment of Pathology, Government Medical College, Kottayam, Thiruvananthapuram, India61911
KrishnaGovindanMulti Disciplinary Research Unit, Government Medical College, Thiruvananthapuram, India61912
KaleeluvilayilChandramohanan NairDepartment of Anatomy, Government Medical College, Thiruvananthapuram, Indiachandramohan1964@yahoo.co.in61913
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31423423
Exposure to Environmental Organic Mercury and Impairments in Human Fertility
195
198
https://www.jri.ir/article/60054
https://www.jri.ir/documents/fullpaper/en/60054.pdf
GeirBjørklundCouncil for Nutritional and Environmental Medicine, Mo i Rana, Norwaybjorklund@conem.org61937
JanAasethInland Norway University of Applied Sciences, Elverum, Norway61938
MaryamDadarRazi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran61939
MonicaButnariuCONEM Romania Biotechnology and Environmental Sciences Group, Banat’s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara, Timisoara, Romania61940
SalvatoreChirumboloDepartment of Neurological and Movement Sciences, University of Verona, Verona, Italy61941