en
1726-7536
1735-8507
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288
gregorian
2020
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19
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online
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fulltext
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33209738
Does Lack of Vertical Transmission of COVID-19 Guarantee the Health of the Fetus or Neonate in Infected Mothers?
229
231
https://www.jri.ir/article/120088
https://www.jri.ir/documents/fullpaper/en/120088.pdf
Mohammad RezaSadeghiReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran محمدرضاصادقیsadeghi@ari.ir77
en
33209739
Detailed Investigation of Downstream TLR Signaling in the Follicular Cells of Women with Endometriosis
<p>Background: Inflammatory responses within the peritoneal cavity may result in endometrial dysfunction in women with endometriosis. The true causes of this disease remain poorly understood. It is hypothesized that downstream toll-like receptors (TLRs) inflammatory cytokines in response to pathogens may be associated with endometriosis. So, this study was aimed at evaluating the expression of TLRs signaling and endometriosis-associated inflammatory responses.<br />
Methods: Totally, 20 infertile endometriosis patients and 20 normal women undergoing controlled ovarian stimulation were enrolled. The cellular pellet and supernatant were obtained by centrifugation of follicular fluid (FF). Evaluation of TLRs and their signaling pathway gene expression was performed on cellular pellets using quantitative-PCR. The supernatant was used for determination of cytokine protein expression by ELISA. The results are expressed as mean±SEM and a p<0.05 was considered statistically significant.<br />
Results: Quantitative-PCR analysis suggested that TLR1, 5, 6, 7, 8, 10, MYD88, NF-ĸB, IL-10 and TGF-β genes expression significantly increased in patients compared to the control group (p<0.05). TLR3, 9, INF-β genes expression was significantly lower in endometriosis than control group (p<0.05). There was no significant difference in the expression of TLR2, TLR4, TIRAP, TRIF, TRAM, and IRF3 between two groups. Also, significant increase in the levels of IL-6, IL-8 and MIF protein in FF of endometriosis group was detected in comparison with normal women (p<0.05).<br />
Conclusion: The expression of TLR downstream signaling in the follicular cells can initiate inflammatory responses and changes in the FF cytokine profile which in turn may induce endometriosis and infertility disorder.</p>
Endometriosis, Follicular cells, Infertility, Inflammation, TLR
231
240
https://www.jri.ir/article/100085
https://www.jri.ir/documents/fullpaper/en/100085.pdf
RezaJafariSchool of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran92073
Seyed AbdolvahabTaghaviDepartment of Gynecology and Obstetrics, School of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran92074
ElhamAmirchaghmaghiDepartment of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iranالهاماميرچقماقي369
RezaSalman YazdiDepartment of Andrology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran92075
LeiliKarimianDepartment of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran92076
MahnazAshrafiDepartment Gynecology and Obstetrics, School of Medicine, Iran University of Medical Sciences, Tehran, Iranمهناز اشرفي72
RezaAflatoonianDepartment of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iranr.aflatoonian@gmail.com1660
en
33209740
Placental Genetic Variants in the Upstream Region of the FLT1 Gene in Pre-eclampsia
<p>Background: Soluble fms-like tyrosine kinase 1 (sFlt-1) is believed to be a prominent component in the pathogenesis of pre-eclampsia, although the precise etiology has remained elusive. In this study, the etiological role of FLT1 variant was further validated in pre-eclampsia by examining this association in a Japanese sample population.<br />
Methods: The genotypes of three variants (rs4769613, rs12050029 and rs149427560) were examined in the upstream region of the FLT1 gene in placentas from pre-eclamptic (n=47) or normotensive control (n=49) pregnancy samples. Additionally, FLT1 mRNA levels in placenta were determined by qRT-PCR. ELISA was further used to detect circulating sFlt-1 levels in maternal sera. The intergroup comparisons were made using the Mann-Whitney U test or one way analysis of variance and P values of less than 0.05 were considered statistically significant.<br />
Results: First, the rs4769613 (C>T) and rs12050029 (G>A) genotypes were examined in placentas but no significant differences were found in the genotype or allele-type frequencies. Next, nearby short tandem repeat, rs149427560, was examined which manifested four size variants. In the genotypewise analysis, the frequency of the 474/476 heterozygote was significantly lower in pre-eclampsia (p<0.05). As expected, the FLT1 mRNA levels were significantly elevated in the pre-eclamptic placentas and sFlt-1 was higher in pre-eclamptic maternal sera. However, the genotype of these variants did not affect the FLT1 mRNA or serum sFlt-1 levels.<br />
Conclusion: Our findings did not support the hypothesis that genetic variations around the FLT1 gene affect the subtle expression changes underlying the etiologic pathway of pre-eclampsia. The hypothesis deserves further investigation through a larger sample size.</p>
FLT1, Placenta, Pre-eclampsia, Short tandem repeat, Single nucleotide variant
240
247
https://www.jri.ir/article/90089
https://www.jri.ir/documents/fullpaper/en/90089.pdf
AkikoOhwakiDivision of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Japan92061
HarukiNishizawaDepartment of Obstetrics and Gynecology, Fujita Health University School of Medicine, Toyoake, Japannharuki@fujitahu.ac.jp92062
AsukaKatoDepartment of Obstetrics and Gynecology, Fujita Health University School of Medicine, Toyoake, Japan92063
TakemaKatoDivision of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Japan92064
JunMiyazakiDivision of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Japan92065
HikariYoshizawaDivision of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Japan92066
YoshiteruNodaDivision of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Japan92067
YoshikoSakabeDivision of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Japan92068
RyokoIchikawaDepartment of Obstetrics and Gynecology, Fujita Health University School of Medicine, Toyoake, Japan92069
TakaoSekiyaDepartment of Obstetrics and Gynecology, Fujita Health University School of Medicine, Toyoake, Japan92070
TakumaFujiiDepartment of Obstetrics and Gynecology, Fujita Health University School of Medicine, Toyoake, Japan92071
HirokiKurahashiDivision of Molecular Genetics, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Japan92072
en
33209741
Estrogenic Effect of Scoparia dulcis (Linn) Extract in Mice Uterus and In Silico Molecular Docking Studies of Certain Compounds with Human Estrogen Receptors
<p>Background: <em>Scoparia dulcis</em> Linn. is reported to be used by women of Assam and Arunachal Pradesh in northeast India for treating menstrual disorders. <em>Scoparia dulcis</em> contains compounds that bind with estrogen receptors (ERα and ERβ) evidenced by increased PCNA in endometrial epithelium.<br />
Methods: Crude extract was orally administered at the dose of 500 mg/kg body weight/day to the female mice (60–70 days old) in five different groups. Each group containing six females included: (I) cyclic control, (II) cyclic extract treated, (III) Ovariectomized (OVX)-vehicle treated (Control), (IV) OVX-E2 treated (V) OVX- extract treated. Extract was administered for eight days to the cyclic groups and three days to the OVX groups. PCNA was detected immunohistochemically in uterine tiss ues and signals were analyzed by Image J software (NIH, USA). Compounds were separated by GC-MS and identified using NIST. In silico molecular docking studies was performed with human estrogen receptors (ERα and ERβ). Molecular dynamics (MD) simulations of the best interacting compound was done using gromacs.<br />
Results: The results showed cell proliferation in the uterine endometrium evidenced by PCNA. Two phytocompounds, Octadecanoic acid and methyl stearate showed binding affinity with ERα and ERβ.<br />
Conclusion: <em>Scoparia dulcis</em> contains compounds having binding affinity with ERα and ERβ. The present study is the first report on compounds from <em>Scoparia dulcis</em> showing binding affinity with human estrogen receptors which may have biological effect on female reproduction.</p>
Endometrium, Estrogen receptors, In silico, Menstrual disorders, Molecular docking, Phytoestrogen, <i>Scoparia dulcis</i> (Linn)
247
259
https://www.jri.ir/article/120087
https://www.jri.ir/documents/fullpaper/en/120087.pdf
KhamheeWangsaDepartment of Zoology, Rajiv Gandhi University, Rono Hills, Itanagar, Arunachal Pradesh, India112070
IndiraSarmaDepartment of Zoology, Rajiv Gandhi University, Rono Hills, Itanagar, Arunachal Pradesh, Indiaisarma14@gmail. com112071
PurbajyotiSaikiaDepartment of Zoology, Rajiv Gandhi University, Rono Hills, Itanagar, Arunachal Pradesh, India112072
DhanabalanAnanthakrishnanCentre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai, India112073
HirendraSarmaDepartment of Zoology, Rajiv Gandhi University, Rono Hills, Itanagar, Arunachal Pradesh, India112074
DevadasanVelmuruganCentre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai, India112075
en
33209742
The Effect of Preincubation Time and Myo-inositol Supplementation on the Quality of Mouse MII Oocytes
<p>Background: It is demonstrated that optimal preincubation time improves oocyte quality, fertilization potential and developmental rate. This study aimed to evaluate the effect of preincubation time in the simple and myo-inositol supplemented medium on the oocyte quality regarding oxidative stress and mitochondrial alteration.<br />
Methods: Cumulus oocyte complexes (COCs) retrieved from superovulated NMRI mice were divided in groups of 0, 4 and 8 <em>hr</em> preincubation time in the simple and 20 <em>mmol/L</em> myo-inositol supplemented media. Intracellular reactive oxygen species (H<sub>2</sub>O<sub>2</sub>), glutathione (GSH), mitochondrial membrane potential (MMP), ATP content, and mitochondrial amount were measured and analyzed in experimental groups. One-way ANOVA and Kruskal-Wallis were respectively used for parametric and nonparametric variables. Statistical significance was defined as p<0.05.<br />
Results: In comparison to control group, variables including ROS, GSH, mitochondrial amount, fertilization and developmental rates were significantly changed after 4 <em>hr</em> of preincubation in the simple medium, while MMP decreased following 8 <em>hr</em> of preincubation in the simple medium (p˂0.001). Preincubation of oocytes up to 8 <em>hr</em> in the simple medium could not decrease ATP content. For both 4 and 8 <em>hr</em> preincubation times, myo-inositole could decrease H<sub>2</sub>O<sub>2</sub> and increase GSH and MMP levels and consequently could improve fertilization rate compared to oocytes preincubated in the simple culture.<br />
Conclusion: It seems that 4 <em>hr</em> or more preincubation time can decrease the oocyte quality and lead to reduced oocyte fertilization and developmental potential. Howevere, myo-inositol may prevent oocyte quality reduction and improve fertilization potential in comparision to the equivalent simple groups.</p>
Developmental rate, Fertilization potential, Mitochondrial alteration, Myo-inositol supplement, Oocyte preincubation time, Oocyte quality, Oxidative stress
259
269
https://www.jri.ir/article/120086
https://www.jri.ir/documents/fullpaper/en/120086.pdf
FatemehMohammadiAnatomy Department, School of Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran112065
MahnazAshrafiCellular and Molecular Research Center, Iran University of Medical Sciences (IUMS), Tehran, Iranمهناز اشرفيAshrafi.m@iums.ac.ir72
ZahraZandiehCellular and Molecular Research Center, Iran University of Medical Sciences (IUMS), Tehran, IranZandieh.z@iums.ac.ir112066
MohammadNajafiBiochemistry Department, School of Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran112067
BehroozNiknafsAnatomy Department, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran322
Fatemeh SadatAmjadiShahid Akbar Abadi Clinical Research Development Unit (ShACRDU), Iran University of Medical Sciences (IUMS), Tehran, Iran112068
MaryamHaghighiAnatomy Department, School of Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran112069
en
33209743
Chromosomal Abnormalities in Couples with Primary and Secondary Infertility: Genetic Counseling for Assisted Reproductive Techniques (ART)
<p>Background: World Health Organization estimates that 60-80 million couple worldwide currently suffer from infertility. Recurrent pregnancy loss (RPL) is also another major concern. Chromosomal rearrangements play a crucial role in primary and secondary infertility and RPL. Underlying genetic abnormalities like chromosomal abnormalities contribute to 5-10% of the reproductive failures. The aim of the study was to evaluate the chromosomal abnormalities in infertility and RPL cases to help obstetrician/fertility experts carry out risk assessment and provide appropriate assisted reproductive techniques for better management of the problem.<br />
Methods: Karyotyping was performed for 414 cases with the history of infertility and RPL over a period of one year. Samples were processed according to procedures of AGT cytogenetic laboratory manual.<br />
Results: Chromosomal abnormalities were observed in 15% of cases. Robertsonian translocation, reciprocal translocation, inversion, derivatives, marker chromosomes, mosaics, aneuploidy and polymorphic variants each contributed 2%, 3%, 3%, 13%, 2%, 10%, 6% and 61%, respectively.<br />
Conclusion: Evaluation of chromosomal abnormalities in couple is warranted prior to planning pregnancy especially for assisted reproductive management cases. Chromosomal analysis can be used as one of the diagnostic tools by OBG/IVF specialists in association with geneticist/genetic counselor for proper reproductive counseling and management.</p>
Banding, Culturing, Heterochromatin, Infertility, Inversion, Polymorphism, Translocation
269
275
https://www.jri.ir/article/80080
https://www.jri.ir/documents/fullpaper/en/80080.pdf
SubhadraPoornimaDepartment of Genetics and Molecular Medicine, Kamineni Hospitals, Hyderabad, IndiaSubhadrapoornima1@gmail.com61992
SwarnalathaDaramDepartment of Genetics and Molecular Medicine, Kamineni Hospitals, Hyderabad, India61993
RamaDevakiDepartment of Biochemistry, Kamineni Academy of Medical Sciences and Research Centre, Hyderabad, India82043
HasanQurratulainDepartment of Genetics and Molecular Medicine, Kamineni Hospitals, Hyderabad, India61995
en
33209744
The Effects of In Vitro Incubation of Asthenoteratozoospermic Semen after Density Gradient Centrifugation at Room Temperature and 37oC on Sperm Parameters, Chromatin Quality and DNA Fragmentation in a Short Time Period
<p>Background: Sperm quality is an important factor in assisted reproductive technology (ART) that affects the success rate of infertile couples treatment. <em>In vitro</em> incubation of sperm can influence its parameters and DNA integrity. The present study focused on the effect of different incubation temperatures sperm parameters on asthenoteratozoospermia semen prepared with density gradient centrifugation at different times.<br />
Methods: Twenty-seven samples were collected and prepared. Then, the suspension was divided into two parts. One part was incubated at room temperature (RT), and another was incubated at 37<sup>o</sup><em>C</em>. Immediately and after 2 <em>hr</em> (2H) and 4 <em>hr</em> (4H), spermatozoa were evaluated regarding motility, viability, morphology, sperm protamine deficiency, chromatin and DNA fragmentation. Statistical analysis was performed using paired t-test and repeated measures. The p<0.05 was considered statistically significant.<br />
Results: Our results showed that following 2 and 4 hr of incubation at RT, sperm progressive motility and viability decreased significantly. Sperm DNA fragmentation increased significantly following 2 and 4 <em>hr</em> of incubation at RT and 37<em><sup>o</sup>C</em>. The Trend analysis confirmed that there were no significant differences between sperm parameters and DNA fragmentation after different times at RT and 37<em><sup>o</sup>C</em>.<br />
Conclusion: Incubation of sperm at RT in comparison to 37<em><sup>o</sup>C</em> didn’t preserve sperm parameters and DNA efficiently. Therefore, IVF, ICSI and IUI procedure should be performed in the soonest possible time after sperm preparation.</p>
Asthenoteratozoospermia, DNA fragmentation, <i>In vitro incubation</i>, Room temperature
275
283
https://www.jri.ir/article/120085
https://www.jri.ir/documents/fullpaper/en/120085.pdf
MotaharehKarimi ZarchiDepartment of Biology, Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Yazd, Iran112061
BehnamMalekiResearch and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran112062
MahmoodDehghani AshkezariDepartment of Biology, Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Yazd, Iran112063
LeilaMotamed ZadehResearch and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran112064
AzamAgha-RahimiResearch and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iranاعظمآقارحیمی63rahimi@gmail.com888
en
33209745
The Association Between Elevated Progesterone Level on Day of hCG Trigger and Live Birth Rates in ART Cycles: A Single Centre Observational Study
<p>Background: The advent of ovarian stimulation within an<em> in vitro</em> fertilization (IVF) cycle has resulted in modifying the physiology of stimulated cycles and has helped optimize pregnancy outcomes. In this regard, the importance of progesterone (P4) elevation at time of human chorionic gonadotrophin (hCG) administration within an IVF cycle has been studied over several decades. Our study aimed to evaluate the association of P4 levels at time of hCG trigger with live birth rate (LBR), clinical pregnancy rate (CPR) and miscarriage rate (MR) in fresh IVF or IVF-ICSI cycles.<br />
Methods: This was a retrospective cohort study (n=170) involving patients attending the Centre for Reproductive and Genetic Health (CRGH) in London. The study cohort consisted of women undergoing controlled ovarian stimulation using GnRH antagonist or GnRH agonist protocols. Univariate and multiple logistic regression analyses were used to evaluate the association of clinical outcomes. Differences were considered statistically significant if p<0.05.<br />
Results: As serum progesterone increased, a decrease in LBR was observed. Following multivariate logistical analyses, LBR significantly decreased with P4 thresholds of 4.0 <em>ng/ml </em>(OR 0.42, 95% CI:0.17-1.0) and 4.5 <em>ng/ml</em> (OR 0.35, 95% CI:0.12-0.96).<br />
Conclusion: P4 levels are important in specific groups and the findings were statistically significant with a P4 threshold value between 4.0-4.5 <em>ng/ml</em>. Therefore, it seems logical to selectively measure serum P4 levels for patients who have ovarian dysfunction or an ovulatory cycles and accordingly prepare the individualized management packages for such patients.</p>
ART, hCG trigger, In vitro fertilization (IVF), Live birth rate (LBR), Ovarian stimulation, Progesterone elevation
283
291
https://www.jri.ir/article/110085
https://www.jri.ir/documents/fullpaper/en/110085.pdf
ShahinRobatiInstitute for Women’s Health, Faculty of Population Health Sciences, University College London, London, United Kingdoms.robati.11@ucl.ac.uk102061
SaabWiamDepartment of Obstetrics and Gynaecology, The American University of Beirut Medical Centre, Beirut, Lebanon102062
MontserratDurán-RetamalInstitute for Women’s Health, Faculty of Population Health Sciences, University College London, London, United Kingdom102063
WaelSaabInstitute for Women’s Health, Faculty of Population Health Sciences, University College London, London, United Kingdom102064
Efstathios TheodorouInstitute for Women’s Health, Faculty of Population Health Sciences, University College London, London, United Kingdom102065
SuzanneCawoodInstitute for Women’s Health, Faculty of Population Health Sciences, University College London, London, United Kingdom102066
PaulSerhalInstitute for Women’s Health, Faculty of Population Health Sciences, University College London, London, United Kingdom41900
SrividyaSeshadriInstitute for Women’s Health, Faculty of Population Health Sciences, University College London, London, United Kingdom102068
en
33209746
Evaluation of Sexual Function Among Infertile Women and Their Sexual Self-Concept
<p>Background: The present study was designed to assess the association between sexual self-concept and sexual function in infertile women.<br />
Methods: A study with a convenience sample of women attending a referral infertility center (Royan Institute) was conducted in Tehran, Iran, in 2017. The Multidimensional Sexual Self-Concept Questionnaire (MSSCQ) and the Female Sexual Function Index (FSFI) were used to collect data. Chi-Square, t-test, Mann-Whitney U test and logistic regression were applied to analyze the data. The significance level was set at p<0.05.<br />
Results: The mean age of participants was 29.7±5.2 years. Overall, 152 women (60.8%) reported that they were experiencing sexual dysfunction. Comparing women with and without sexual dysfunction, there were significant differences between two groups on most measures such as sexual anxiety, sexual motivation, sexual satisfaction, and sexual depression (p<0.05). However, the results obtained from logistic regression indicated that women’s and husband’s age (OR for women’s age=1.26, 95% CI=1.10-1.44, p<0.001; OR for husband’s age=0.86, 95% CI=0.77-0.97, p=0.014), cause of infertility (OR for female factor=9.17, 95% CI=2.26-37.2, p=0.002; OR for male factor=3.90, 95% CI=1.26-12.1, p=0.018; OR for male and female factor=3.57, 95% CI=1.12-11.4, p=0.032), sexual motivation (OR=0.35, 95% CI=0.16-0.75, p=0.007) and sexual satisfaction (OR=0.23, 95% CI=0.09-0.56, p=0.001) were significantly associated with sexual dysfunction.<br />
Conclusion: The findings suggest that sexual motivation and sexual satisfaction are important dimensions of sexual self-concept in infertile women. Indeed, it is essential to inform policy makers and stakeholders to provide more sexual health support for this population in the process of treatment.</p>
Infertility, Sexual dysfunction, Sexual health, Sexual self-concept
291
298
https://www.jri.ir/article/90088
https://www.jri.ir/documents/fullpaper/en/90088.pdf
HedyehRiaziDepartment of Midwifery and Reproductive Health, School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, Iran82069
HajarLotfollahiStudents Research Office, School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, Tehran, Iran82070
RezaOmani-SamaniDepartment of Epidemiology and Reproductive Health, Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, Tehran, Iran82071
SamanMaroufizadehDepartment of Biostatistics, School of Nursing and Midwifery, Guilan University of Medical Sciences, Rasht, Iran82072
AliMontazeriPopulation Health Research Group, Health Metrics Research Center, Iranian Institute for Health Sciences Research, ACECR, Tehran, Iran82073
en
33209747
Intra-individual Genomic Variation in Tissues (Blood vs. Testis) Through SNP Microarray: A Case report of Two Patients with Idiopathic Sertoli Cell Only Syndrome (SCOS)
<p>Background: Sertoli cell only syndrome (SCOS) or germ cell aplasia is characterized by the existence of only sertoli cells in the seminiferous tubule without any germ cells. SCOS is a multifactorial disorder but genetic factors play a major role in pathogenesis of idiopathic SCOS.<br />
Case Presentation: Two cases of idiopathic SCOS had been reported with no non-genetic factor in their medical history that could play a role in aetiology of SCOS. Also, two normal fertile males were recruited as controls in this study. For evaluation of genomic imbalance, karyotyping (G-banding), FISH, STS-PCR and SNP microarray were carried out. SNP microarray was carried out in DNA of peripheral blood for cases as well as controls. However, for cases, SNP microarray was conducted in DNA of testicular Fine needle aspiration cytology (FNAC).<br />
Conclusion: No chromosome abnormality and Yq microdeletion was found in cases as well as in controls. Microarray detected many CNVs and LOH that cover genes with spermatogenesis related function and PAR CNVs in both cases. Differential genomic variations were found in blood and testis for cases. Therefore, the evaluation of pathogenesis of idiopathic SCOS might be dependent on both tissue samples. The evaluation of genomic imbalances at both tissue levels should be done for a large cohort of patients.</p>
Copy number variations, Loss of heterozygosity, Sertoli cell only syndrome, Single nucleotide polymorphisms
298
308
https://www.jri.ir/article/90087
https://www.jri.ir/documents/fullpaper/en/90087.pdf
AiyushSharmaDepartment of Reproductive Biology, All India Institute of Medical Sciences, New Delhi, India82065
AshutoshHalderDepartment of Reproductive Biology, All India Institute of Medical Sciences, New Delhi, India82066
SeemaKaushalDepartment of Pathology, All India Institute of Medical Sciences, New Delhi, India82067
ManishJainDepartment of Reproductive Biology, All India Institute of Medical Sciences, New Delhi, Indiaom_704@yahoo.co.in82068
en
33209748
A Novel De Novo Chromosomal Insertion, 46 XX, ins(7:13)(p14; q14.2q21.1) is Related to the Embryo Development Arrest Following Assisted Reproductive Technique
<p>Background: Infertility is a problem affecting a large number of couples in the world. One of the causes of infertility can be chromosomal rearrangements such as insertions. In this case report study, the outcome of two intra-cytoplasmic sperm injection (ICSI) cycles of an infertile woman with de novo chromosomal insertion is explained.<br />
Case Presentation: A couple with a 10-year history of infertility referred to our infertility clinic. The husband had a daughter in his first previous marriage. The wife had a 7 and a 10 year history of infertility in the first and second marriages, respectively. In the first marriage, she reported a history of 2 failed intra-uterine insemination (IUI) cycles. In the second marriage, she had a history of 1 spontaneous abortion at 12 weeks of pregnancy, 4 failed IUI cycles, and 1 failed ICSI cycle. The couple was subjected to ICSI cycles twice and failed due to embryo development arrest. The couple referred for karyotyping. The husband showed a normal male karyotype. In comparison, the wife revealed an abnormal female karyotype with two rearrangements: chromosome 13 with an interstitial deletion between bands q14.2 and q21.1, and a derivative chromosome 7 containing this segment of chromosome 7 as an insertion onto short arm at the p14 position.<br />
Conclusion: To the best of our knowledge, this is the first report of insertion 46 XX, ins(7:13)(p14; q14.2q21.1) which is associated with the embryo development arrest following assisted reproductive technique.</p>
Assisted reproductive technique, Chromosomal rearrangement, Cytogenetic analysis, Infertility woman, IVF failure
308
312
https://www.jri.ir/article/90085
https://www.jri.ir/documents/fullpaper/en/90085.pdf
AzamAzargoonDepartment of Infertility, Amir-AL-Momenin Hospital, Semnan University of Medical Sciences, Semnan, Iran82061
NahidAzadDepartment of Reproductive Biology, Semnan University of Medical Sciences, Semnan, Irannazad1390@gmail.com31908
en
33209749
Infertility Distress Management in Couples Treated with Assisted Reproductive Techniques (ART) in COVID-19 Pandemic
312
314
https://www.jri.ir/article/90086
https://www.jri.ir/documents/fullpaper/en/90086.pdf
FatemehHamidiM.Sc., Student of Midwifery Counseling, Student Research Committee, Department of Midwifery, Nasibeh School of Nursing and Midwifery, Mazandaran University of Medical Sciences, Sari, Iran82062
FarzanehBabapourM.Sc., Student of Midwifery Counseling, Student Research Committee, Department of Midwifery, Nasibeh School of Nursing and Midwifery, Mazandaran University of Medical Sciences, Sari, Iran82063
ZeinabHamzehgardeshiSexual and Reproductive Health Research Center, Department of Reproductive Health and Midwifery, School of Nursing and Midwifery, Mazandaran University of Medical Sciences, Sari, IranZ.hamzehgardeshi@mazums.ac.ir82064