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34900634
The Disputable Discourse on Accuracy and Effectiveness of PGT-A in Light of Advancements in Genetic Tools
149
151
https://www.jri.ir/article/120128
https://www.jri.ir/documents/fullpaper/en/120128.pdf
Mohammad RezaSadeghiReproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran محمدرضاصادقیsadeghi@ari.ir77
en
34900635
Evaluating the Expression of Wnt Pathway Related Genes in Mouse Vitrified Preantral Follicles: An Experimental Study
<p>Background: Wnt signaling pathway plays critical role in ovarian follicle development. Therefore, the aim of this study was to evaluate the effects of vitrification on the expression of Wnt pathway related genes in preantral follicles (PFs).<br />
Methods: Isolated PFs (n=982) of 14-16 day old female mice (n=45: 15 for each group) were divided into fresh (n=265), toxicity (n=272), and vitrified (n=265). The mRNA levels of Wnt2, Wnt4, Lrp5 and Fzd3 were evaluated by real-time PCR on the 2nd and 6th days of culture period. One-way ANOVA was conducted to analyze the data. Post hoc Tukey's HSD was used for multiple comparisons and p-value less than 0.05 was considered statistically significant.<br />
Results: The developmental parameters of fresh PFs were significantly higher than those of vitrified (p<0.001). There were no differences between fresh and vitrified PFs on the 2nd day of culture (p<0.001). Wnt4 expression levels decreased significantly in vitrified groups compared with fresh ones (p<0.001). Fzd3 and Lrp expression levels increased significantly in vitrified groups compared with those in the fresh group on the 2nd day (p<0.001). On the 6th day of culture period, the expression levels of Wnt2 and Fzd3 increased significantly in vitrified group compared to those of fresh group (p<0.001). Moreover, the expression levels of Wnt4 and Lrp increased significantly in toxicity groups compared to those of the control group (p<0.001).<br />
Conclusion: Vitrification increase the expression levels of Wnt2, Lrp and Fzd3 genes of PFs during in vitro culture.</p>
Cryopreservation, Folliculogenesis, Ovary, Wnt/β-catenin pathway
151
159
https://www.jri.ir/article/120121
https://www.jri.ir/documents/fullpaper/en/120121.pdf
ShahlaBabakiSchool of Biology, Damghan University, Damghan, Iran112231
SaeedZavarehInstitute of Biological Sciences, Damghan University, Damghan, IranSaeedZavarehzavareh.s@du.ac.ir1171
ParisaFarrokInstitute of Biological Sciences, Damghan University, Damghan, Iran112232
MeysamNasiriInstitute of Biological Sciences, Damghan University, Damghan, Iran112233
en
34900636
The Association of Kinetic Variables with Blastocyst Development and Ploidy Status
<p>Background: Despite a plethora of studies conducted so far, a debate is still unresolved as to whether TLM can identify predictive kinetic biomarkers or algorithms universally applicable. Therefore, this study aimed to elucidate if there is a relationship between kinetic variables and ploidy status of human embryos or blastocyst developmental potential.<br />
Methods: For conducting this retrospective cohort study, the normal distribution of data was verified using Kolmogorov-Smirnov test with the Lilliefors’ amendment and the Shapiro-Wilk test. Kinetic variables were expressed as median and quartiles (Q1, Q2, Q3, Q4). Mann-Whitney U-test was used to compare the median values of parameters. Univariate and multiple logistic regression models were used to assess relationship between blastocyst developmental potential or ploidy status and kinetics. Several confounding factors were also assessed.<br />
Results: Blastocyst developmental potential was positively correlated with the t4-t3 interval (s2) (OR=1.417, 95% CI of 1.288-1.560). s2 median value was significantly different between high- and low-quality blastocysts (0.50 and 1.33 hours post-insemination, <em>hpi</em>, respectively; p=0.003). In addition, timing of pronuclear appearance (tPNa) (OR=1.287; 95% CI of 1.131-1.463) had a significant relationship with ploidy changes. The median value of tPNa was statistically different (p=0.03) between euploid and aneuploid blastocysts (Euploid blastocysts=8.9 <em>hpi</em>; aneuploid blastocysts=10.3 <em>hpi</em>).<br />
Conclusion: The present findings are in line with the study hypothesis that kinetic analysis may reveal associations between cleavage patterns and embryo development to the blastocyst stage and ploidy status.</p>
Aneuploidy, Blastocyst, Embryo, Embryonic development, Microscopy
159
165
https://www.jri.ir/article/120115
https://www.jri.ir/documents/fullpaper/en/120115.pdf
FrancescaPennettaUOC Laboratory Medicine AULSS 5 Polesana, Rovigo, Italy112202
CristinaLagallaBaby Family and Fertility Center, Bologna, Italy112203
RaffaellaSciajnoBaby Family and Fertility Center, Bologna, Italy112204
NicolettaTarozziBaby Family and Fertility Center, Bologna, Italy112205
MarcoNadaliniBaby Family and Fertility Center, Bologna, Italy112206
CarlottaZacàBaby Family and Fertility Center, Bologna, Italy112207
GiovanniCoticchioBaby Family and Fertility Center, Bologna, Italygiovanni.coticchio@9puntobaby.it112208
AndreaBoriniBaby Family and Fertility Center, Bologna, Italy112209
en
34900637
Correlation of Sperm Mitochondrial DNA 7345 bp and 7599 bp Deletions with Asthenozoospermia in Jordanian Population
<p>Background: Alterations in sperm mitochondrial DNA (mtDNA) affect the functions of some OXPHOS proteins which will affect sperm motility and may be associated with asthenozoospermia. The purpose of this study was to investigate the correlation between 7599-<em>bp </em>and 7345-<em>bp</em> sperm mtDNA deletions and asthenozoospermia in Jordan.<br />
Methods: Semen specimens from 200 men including 121 infertile and 79 healthy individuals were collected at the Royal Jordanian Medical Services In-vitro fertilization (IVF) units. The mtDNA was extracted followed by mtDNA amplification. Polymerase chain reaction (PCR) was conducted for the target sequences, then DNA sequencing was performed for the PCR products. Chi-square, Fisher's and Spearman's tests were used to calculate the correlation.<br />
Results: The results showed a significant correlation between the presence of 7599-<em>bp</em> mtDNA deletion and infertility where the frequency of the 7599-<em>bp</em> deletion was 63.6 % in the infertile group compared to the fertile 34.2% (p<0.001, (OR=3.37, 95% CI=1.860 to 6.108)). Additionally, the sperm motility showed a significant association with the frequency of the 7599-<em>bp</em> deletion (p=0.001, r=-0.887). The 7345-<em>bp</em> mtDNA deletion showed no assoctiation with the infertility (p=0.65, (OR=0.837, 95% CI=0.464-1.51)) or asthenozoospermia (p=0.98, r=0.008).<br />
Conclusion: We demonstrated a significant correlation between asthenozoospermia and the 7599-<em>bp</em> mtDNA deletion but not the 7345-bp mtDNA deletion in the infertile men in Jordan. Screening for deletions in sperm mtDNA can be used as a pre-diagnostic molecular marker for male infertility.</p>
Asthenozoospermia, Gene deletion, Male infertility, Mitochondrial DNA, Sperm motility
165
173
https://www.jri.ir/article/120113
https://www.jri.ir/documents/fullpaper/en/120113.pdf
MazharAl ZoubiDepartment of Basic Medical Sciences, Faculty of Medicine, Yarmouk University, Irbid, Jordanmszoubi@yu.edu.jo112189
Ali M.Al-TalafhaDepartment of Biological Sciences, Faculty of Science, Yarmouk University, Irbid, Jordan112190
EmadAl SharuKing Hussein Medical Centre, Royal Medical Services, Amman, Jordan112191
BahaaAl-TradDepartment of Biological Sciences, Faculty of Science, Yarmouk University, Ibadan, Jordan112192
AymanAlZu'biDepartment of Basic Medical Sciences, Faculty of Medicine, Yarmouk University, Irbid, Jordan112193
ManalAbuAlarjahDepartment of Basic Medical Sciences, Faculty of Medicine, Yarmouk University, Irbid, Jordan112194
QasemShehabDepartment of Clinical Sciences, Faculty of Medicine, Yarmouk University, Irbid, Jordan112195
MohammadAlsmadiDepartment of Gynecology and Reproductive Medicine, Faculty of Medicine, Saarland University, Saarbrücken, Germany112196
Khalid M.Al-BataynehDepartment of Biological Sciences, Faculty of Science, Yarmouk University, Irbid, Jordan112197
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34900638
Dietary Fatty Acid Intakes and the Outcomes of Assisted Reproductive Technique in Infertile Women
<p>Background: The purpose of this study was evaluating the relationship between fatty acid (FA) intakes and the Assisted Reproductive Technique (ART) outcomes in infertile women.<br />
Methods: In this descriptive longitudinal study, a validated food frequency questionnaire (FFQ) was used to measure dietary intakes among 217 women with primary infertility seeking ART treatments at Isfahan Fertility and Infertility Center, Isfahan, Iran. The average number of total and metaphase II (MII) oocytes, the fertilization rate, the ratio of good and bad quality embryo and biochemical and clinical pregnancy were assessed. Analyses were performed using mean, standard deviation, Chi-square test, ANOVA, ANCOVA, logistic regression.<br />
Results: A total of 140 women were finally included in the study. There was a positive relationship between the average number of total and MII oocytes and the amount of total fatty acids (TFAs), saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), polyunsaturated fatty acids (PUFAs), linoleic acids, linolenic acids, and oleic acids intakes, while eicosapentaenoic acids (EPAs) and docosahexaenoic acids (DHAs) intakes had an inverse relationship. Consuming more amounts of TFAs, SFAs, PUFAs, MUFAs, linoleic acids, and oleic acids was associated with the lower fertilization rate, whereas the consumption of linolenic acids and EPAs increased the fertilization rate. The ratio of good quality embryo was directly affected by the amount of PUFAs intakes. Additionally, there was a negative correlation between the amount of SFAs intakes and the number of pregnant women.<br />
Conclusion: TFAs, SFA, PUFA, and MUFA intakes could have both beneficial and adverse impacts on ART outcomes.</p>
Assisted reproductive technique, Dietary fats, In Vitro Fertilization, Infertility, Nutrition assessment
173
184
https://www.jri.ir/article/120114
https://www.jri.ir/documents/fullpaper/en/120114.pdf
MaryamJahangirifarSchool of Nursing and Midwifery, Faculty of Medicine, Nursing and Health Sciences, Monash University, Melbourne, Australia112199
MahboubehTaebiDepartment of Midwifery and Reproductive Health, Isfahan University of Medical Sciences, Isfahan, Iranm_taebi@nm.mui.ac.ir112198
Mohammad HosseinNasr-EsfahaniDepartment of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran168
MotaharHeidari-BeniChild Growth and Development Research Center, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran112200
Gholam HosseinAsgari Department of Community Nutrition, Isfahan University of Medical Sciences, Isfahan, Iran112201
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34900639
Women’s Reports of Barriers to and Facilitators of Oral Medication Adherence During Ovarian Stimulation: A Mixed Methods Pilot Study
<p>Background: Adherence to lifestyle modification recommendations remains problematic for women undergoing fertility treatment, raising concerns about the extent to which women adhere to prescribed medication regimens. Limited data have shown suboptimal oral medication adherence rates of 19% to 74%. The objective of this study was to explore what women perceive as barriers to and facilitators of oral medication adherence during fertility treatment cycles.<br />
Methods: An exploratory mixed methods pilot study was conducted among a sample of 30 women who were actively taking one to two cycles of letrozole or clomiphene citrate for ovarian stimulation in conjunction with intrauterine insemination cycles. Medication adherence barriers were measured using a 20-item survey. Medication adherence facilitators and personal experiences with fertility treatment were assessed with structured interviews. Medication adherence was assessed with electronic event monitoring.<br />
Results: The overall medication adherence median was 0.97 with a range of 0.75 to 1.00, and nine women (50%) demonstrated perfect adherence. The most commonly reported barriers were recently feeling sad, down, or blue (53%), and taking medication more than once per day (40%). Women with higher barrier scores had significantly lower medication adherence scores (p=0.02) compared to women with lower total barrier scores. Facilitators included using physical aides as reminders (60%) and establishing a daily routine (50%). No significant correlation was found between medication adherence scores and facilitators.<br />
Conclusion: The dynamic interplay between perceived barriers and facilitators and women’s medication-taking patterns could influence whether or not medication regimens are followed correctly.</p>
Female infertility, Ovarian stimulation, Psychology
184
201
https://www.jri.ir/article/120112
https://www.jri.ir/documents/fullpaper/en/120112.pdf
Diane EMahoneySchool of Nursing, University of Kansas Medical Center, Kansas, USdmahoney@kumc.edu 112187
Cynthia LRussellSchool of Nursing, University of Kansas Medical Center, Kansas, US112188
en
34900640
Multiple Mutations in Exon-2 of Med-12 Identified in Uterine Leiomyomata
<p>Background: Uterine leiomyomata (UL), commonly known as uterine fibroids, are benign smooth muscle tumors of the myometrium. They cause pelvic pain, abnormal uterine bleeding, and infertility in women of reproductive age. The ovarian hormone estrogen is the main stimulator for the fibroid growth. The etiology is not yet clearly understood; however, UL are believed to be monoclonal tumors arising from a common progenitor cell. Chromosomal cytogenetic abnormalities have been demonstrated in 40-50% of the fibroids. The most frequent tumor specific genetic alterations in UL were identified in exon-2 of Mediator Complex Subunit 12 (MED-12).<br />
Methods: In the present study, twenty-two multiple fibroids were evaluated both from the same uterus and from different uteri, of four women, for somatic mutations in hotspot region of MED-12. The tissue DNA of the UL's was isolated, amplified by PCR visualized on gel and sent for Sanger sequencing.<br />
Results: The results indicate several variants in exon-2 and flanking intronic regions, seven exonic variants and five intronic variants which provide evidence that multiple UL in the same uterus may not be clonal in origin.<br />
Conclusion: This study indicates genetic heterogeneity. UL may not have a clonal origin, these exon-2 variants of MED-12 gene could be involved in UL progression.</p>
Clonal, Codon 44, Gene variants, Mediator Complex Subunit 12, Somatic mutations, Uterine Leiomyoma
201
210
https://www.jri.ir/article/120120
https://www.jri.ir/documents/fullpaper/en/120120.pdf
RuqiaFirdausDepartment of Genetics, Osmania University, Hyderabad, Indiaruqiafirdaus@gmail.com112227
PrabhaAgrawalDepartment of Gynaecology and Obstetrics, Medicover Hospitals, Hi–Tech City, Hyderabad, India112228
ManjulaAnaganiDepartment of Gynaecology and Obstetrics, Medicover Hospitals, Hi–Tech City, Hyderabad, India112256
KodatiVijayalakshmiDepartment of Genetics, Osmania University, Hyderabad, India112229
QurratulainHasanDepartment of Genetics and Molecular Medicine, Kamineni Hospital, LB Nagar, Hyderabad, India112230
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34900641
In Silico Analysis of Sperm From Ejaculates with Different Semen Characteristics
<p>Background: Male infertility is associated with altered characteristics of the sperm within the ejaculate. It is possible to find molecular explanations for the observed phenotypes and their consequences. This study aimed to analyze, using a specialized software, a gene set of transcriptomic data from different types of ejaculates.<br />
Methods: Data from ejaculate samples categorized as normal, oligospermia, and teratozoospermia were obtained from Gene Expression Omnibus (GEO). After normalization, the data average for each sample category was calculated and analyzed independently using Ingenuity Pathway Analysis (IPA).<br />
Results: Five important canonical pathways are involved in normal and altered semen samples (Oligospermia and teratozoospermia) except sirtuin signaling and mitochondrial dysfunction pathways. The five most important biological processes are identified in all semen phenotypes, but the only difference is the genes connected with initiation of RNA transcription in oligospermic and asthenospermic samples.<br />
Conclusion: Surprisingly, different types of ejaculates share many pathways and biological processes; sperm proteomics as a new global approach gives clues for the development of strategies to explain the reason for observed phenotypes of ejaculated spermatozoa, their possible effect on fertility, and for implementing research strategies in the context of infertility diagnosis and treatment.</p>
Fertility, Male, RNA, Spermatozoa, Transcriptome
210
216
https://www.jri.ir/article/120122
https://www.jri.ir/documents/fullpaper/en/120122.pdf
Jesús AlfredoGutiérrezReproduction Group, Department of Microbiology and Parasitology, Medical School, University of Antioquia, Antioquia, Colombia112234
Walter DMayaReproduction Group, Department of Microbiology and Parasitology, Medical School, University of Antioquia, Antioquia, Colombiawdario.cardona@udea.edu.co112235
en
34900642
Persistent Mullerian Duct Syndrome: A Rare Case of an Adult Infertile Male with Bilateral Cryptorchidism
<p>Background: Persistent mullerian duct syndrome (PMDS) is a very rare form of internal male pseudohermaphroditism in individuals who are phenotypically males with 46 XY karyotypes harbouring internal female reproductive organs which are Mullerian derivatives. It occurs as a defect in the genes coding for the Mullerian inhibiting substance (MIS) or the anti Mullerian hormone (AMH) receptor, ultimately leading to failure of regression of Mullerian ducts.<br />
Case Presentation: A 29-year-old male with PMDS presented with complaints of primary infertility. Diagnosis was made with the help of high index of suspicion, radiological imaging, and karyotyping. Our patient underwent exploratory laparotomy with hysterectomy and bilateral orchidopexy.<br />
Conclusion: The purpose of this study was increasing awareness regarding rare entities and surgeons should have high clinical suspicion of PMDS when patient with bilateral undescended testis comes for the evaluation of primary infertility.</p>
Anti Mullerian hormone (AMH), Cryptorchidism, Hysterectomy, Karyotyping, Mullerian inhibiting substance (MIS), Orchidopexy, Persistent mullerian duct syndrome (PMDS), Primary infertility
216
220
https://www.jri.ir/article/120119
https://www.jri.ir/documents/fullpaper/en/120119.pdf
PrakashSankapalDepartment of Urology, Grant Government Medical College and Sir JJ Hospital, Mumbai, India112222
VenkatGiteDepartment of Urology, Grant Government Medical College and Sir JJ Hospital, Mumbai, Indiabalajigite@yahoo.com112223
AgrawalMayankDepartment of Urology, Grant Government Medical College and Sir JJ Hospital, Mumbai, India112224
MaheshSaneDepartment of Urology, Grant Government Medical College and Sir JJ Hospital, Mumbai, India112225
AtulSingalDepartment of Urology, Grant Government Medical College and Sir JJ Hospital, Mumbai, India112226
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34900643
Successful Management of Caesarean Scar Ectopic Pregnancies: A Report of Five Cases
<p>Background: Cesarean section scar ectopic pregnancy (CSEP) is a rare and poten-tially life-threatening condition. A standardized management protocol is yet to be established owing to limited data available.<br />
Case Presentation: In this paper, five cases of CSEP over a period of 18 months at a tertiary referral hospital, managed medically with methotrexate administered both systemically and into the gestational sac at the time of feticide with potassium chloride (KCL) are presented. Surgical management was the second line therapy when medical treatment failed.<br />
Conclusion: With rising trends in cesarean deliveries, CSEP may be a challenge which requires close investigation regarding its diagnosis and treatment on the merits of case studies and available healthcare facilities.</p>
Caesarean section, Ectopic pregnancy, Gestational sac, Methotrexate
220
225
https://www.jri.ir/article/120127
https://www.jri.ir/documents/fullpaper/en/120127.pdf
NeenaMalhotraDepartment of Obstetrics and Gynaecology, All India Institute of Medical Sciences, New Delhi, India51907
NilofarNoorDepartment of Obstetrics and Gynaecology, All India Institute of Medical Sciences, New Delhi, India112253
PiyushBansalDepartment of Obstetrics and Gynaecology, All India Institute of Medical Sciences, Nagpur, India112254
K. AparnaSharmaDepartment of Obstetrics and Gynaecology, All India Institute of Medical Sciences, New Delhi, Indiakaparnasharma@gmail.com112255