en
1726-7536
1735-8507
69
2139
288
gregorian
2021
8
2
22
3
online
1
fulltext
en
34900636
The Association of Kinetic Variables with Blastocyst Development and Ploidy Status
<p>Background: Despite a plethora of studies conducted so far, a debate is still unresolved as to whether TLM can identify predictive kinetic biomarkers or algorithms universally applicable. Therefore, this study aimed to elucidate if there is a relationship between kinetic variables and ploidy status of human embryos or blastocyst developmental potential.<br />
Methods: For conducting this retrospective cohort study, the normal distribution of data was verified using Kolmogorov-Smirnov test with the Lilliefors’ amendment and the Shapiro-Wilk test. Kinetic variables were expressed as median and quartiles (Q1, Q2, Q3, Q4). Mann-Whitney U-test was used to compare the median values of parameters. Univariate and multiple logistic regression models were used to assess relationship between blastocyst developmental potential or ploidy status and kinetics. Several confounding factors were also assessed.<br />
Results: Blastocyst developmental potential was positively correlated with the t4-t3 interval (s2) (OR=1.417, 95% CI of 1.288-1.560). s2 median value was significantly different between high- and low-quality blastocysts (0.50 and 1.33 hours post-insemination, <em>hpi</em>, respectively; p=0.003). In addition, timing of pronuclear appearance (tPNa) (OR=1.287; 95% CI of 1.131-1.463) had a significant relationship with ploidy changes. The median value of tPNa was statistically different (p=0.03) between euploid and aneuploid blastocysts (Euploid blastocysts=8.9 <em>hpi</em>; aneuploid blastocysts=10.3 <em>hpi</em>).<br />
Conclusion: The present findings are in line with the study hypothesis that kinetic analysis may reveal associations between cleavage patterns and embryo development to the blastocyst stage and ploidy status.</p>
Aneuploidy, Blastocyst, Embryo, Embryonic development, Microscopy
159
165
https://www.jri.ir/article/120115
https://www.jri.ir/documents/fullpaper/en/120115.pdf
FrancescaPennettaUOC Laboratory Medicine AULSS 5 Polesana, Rovigo, Italy112202
CristinaLagallaBaby Family and Fertility Center, Bologna, Italy112203
RaffaellaSciajnoBaby Family and Fertility Center, Bologna, Italy112204
NicolettaTarozziBaby Family and Fertility Center, Bologna, Italy112205
MarcoNadaliniBaby Family and Fertility Center, Bologna, Italy112206
CarlottaZacĂ Baby Family and Fertility Center, Bologna, Italy112207
GiovanniCoticchioBaby Family and Fertility Center, Bologna, Italygiovanni.coticchio@9puntobaby.it112208
AndreaBoriniBaby Family and Fertility Center, Bologna, Italy112209