<p>Background: In Brazil, donor anonymity is mandatory; however, the tendency of Brazilians towards the practice is unknown. In this study, an attempt was made to investigate whether couples undergoing assisted reproductive technology (ART) have a different perception of anonymous versus identity-release gamete donation than a target population in Brazil.<br /> Methods: This cross-sectional study was performed from September 1, 2020 to December 15, 2020. For that purpose, surveys through online platforms were conducted, including either patients undergoing ART (ART-group, n=400) or subjects interested in the theme (interested-group, n=100) randomized by age at a 1:4 ratio. The survey collected information on the participants&rsquo; attitudes towards anonymity of gamete donors, and answers were compared between the groups.<br /> Results: Most participants stated that the relationship between children and their parents would be affected by the child&#39;s knowledge of the origin of its conception. Most participants in the ART-group believed that the gamete donor&rsquo;s identity should not be revealed to the child, while only half of the interested-group stated the same. Most of the participants stated that &quot;the donor&#39;s identity should be revealed if the child questions its biological origin&quot;. &quot;From birth&quot; was the second most common response, while &quot;when the child turns 18 years old&quot; and &quot;sometime during teenage years&quot; were less common answers.<br /> Conclusion: The attitudes of ART patients about anonymity are conservative, with most participants believing that family relationships may be affected if the child is aware of the origin of his/her conception. These patients also believe that the identity of the gamete donor should not be revealed to the child.&nbsp;</p>