J Reprod Infert

arij001

Journal of Reproduction & Infertility

2228-5482 2251-676X

Avicenna Research Institute

jri30033

The Study of Association Between Polymorphism of TNF-α Gene’s Promoter Region and Recurrent Pregnancy Loss

Aboutorabi

Roshanak

Infertility Laboratory, Beheshti Hospital, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Behzadi

Ehsan

School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Sadegh

Mohammad Javad

School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Fatehi

Seyed Pooriya

School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Semsarzadeh

Soroosh

School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Zarrin

Yasaman

School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Kazemi

Mohammad

Department of Genetics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Rafiee

Laleh

Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Mostafavi

Fatemeh Sadat

Infertility Laboratory, Beheshti Hospital, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

19 4 211 219 24 4 2018 12 8 2018

<p>Background: According to the literature review, polymorphisms of tumor necrosis factor alpha’s (TNF-α) promoter region are probably the genetic risk factors of recurrent pregnancy loss. This study has investigated five single nucleotide polymorphisms in the TNF-α gene’s promoter region to evaluate their relationship with recurrent pregnancy loss disorder.<br /> Methods: Blood samples were taken from 65 women with recurrent pregnancy loss (Case group) and 65 healthy women with a history of successful pregnancy (Control group). Polymerase chain reaction with high resolution melting (PCR-HRM) analysis was done to determine the promoter region of -308G/A, -850T/C, -238G/A, -1031T/C and -863A/C TNF-α polymorphisms. The data were assessed using logistic regression models. P values less than 0.05 were considered statistically significant.<br /> Results: Significant associations were found between recurrent pregnancy loss and -863C/A (p=0.000), -308G/A (p=0.045), and -238G/A (p=0.034) polymorphisms. TNF-α polymorphisms of -863C and -238G may be susceptible factors of recurrent pregnancy loss cases. The -308G polymorphism has an important role in maintaining pregnancy.<br /> Conclusion: The -863C/A and -238G/A TNF-α polymorphisms are possible genetic risk factors of recurrent pregnancy loss and might be its predictive markers.</p>