J Reprod Infert arij001 Journal of Reproduction & Infertility 2228-5482 2251-676X Avicenna Research Institute jri60051 Impact of Genetic Variants in Estrogen Receptor-β Gene in the Etiology of Uterine Leiomyomas BharathiChitrojuInstitute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, Telangana, IndiaAnupamaDesamalaDepartment of Gynaecology, Modern Government Maternity Hospital, Hyderabad, Telangana, IndiaPratibhaNallariInstitute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, Telangana, IndiaVenkateshwariAnantapurInstitute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, Telangana, India 20 3 151 161 30 11 2018 17 4 2019

<p>Background: Uterine leiomyomas are steroid hormone dependent myometrial neoplasms of female genital tract which appear after menarche and regress at menopause. The present study evaluated the role of ER-&beta; gene polymorphisms (rs3020449 C/T, rs3020450 G/A, rs1271572 G/T, rs1256049 G/Aand rs4986938 G/A) in the etiology of disease.&nbsp;<br /> Methods: A total of 150 clinically, ultrasonographically evaluated uterine leiomyoma patients and an equal number of individuals as controls were considered for the present study. Genotype analysis was carried out by TETRA Primer Amplification Refractory Mutation System&ndash;PCR for promoter polymorphisms and PCR- RFLP method was done for exonic polymorphisms followed by agarose gel electrophoresis. The strength of the association of ER-&beta; gene polymorphisms between controls and patients were measured by appropriate statistical methods.<br /> Results: An increased frequency of T/T genotype and T allele of rs3020449, AA genotype and A allele of rs3020450, T/T genotype and T allele of rs1271572, AA genotype and A allele of rs1256049 and A/A genotype and A allele of rs4986938 was observed in cases when compared with controls.<br /> Conclusion: The study indicates that the ER-&beta; gene polymorphisms may act as a major genetic regulator in the etiology of uterine leiomyomas.</p>