J Reprod Infert

arij001

Journal of Reproduction & Infertility

2228-5482 2251-676X

Avicenna Research Institute

jri60060

NUCB2/Nesfatin-1 in the Blood and Follicular Fluid in Patients with Polycystic Ovary Syndrome and Poor Ovarian Response

Catak

Zekiye

Department of Clinical Biochemistry, University of Health Sciences, Elazig Fethi Sekin City Hospital, Elazig, Turkey

Yavuzkir

Seyda

Department of Obstetrics and Gynecology, School of Medicine, Firat University, Elazig, Turkey

Kocdemir

Esra

Department of Clinical Biochemistry, Kovancilar State Hospital, Taipei City, Turkey

Ugur

Kader

Department of Internal Medicine (Endocrinology and Metabolism Diseases), School of Medicine, Firat University, Elazig, Turkey

Yardim

Meltem

Department of Medical Biochemistry and Clinical Biochemistry (Firat Hormones Research Group), Medical School, Firat University, Elazig, Turkey

Sahin

İbrahim

Department of Medical Biology, Medical School, Erzincan Binali Yildirim University, Elazig, Turkey

Agirbas

Esra

School of Medicine, Medical School Student, Firat University, Elazig, Turkey

Aydin

Suleyman

Department of Medical Biochemistry and Clinical Biochemistry (Firat Hormones Research Group), Medical School, Firat University, Elazig, Turkey

20 4 225 231 29 1 2019 7 5 2020

Background: Failure to respond adequately to standard protocols and to recruit adequate follicles is called ‘poor ovarian response’. The relationships between metabolic alterations and NUCB2/Nesfatin-1 levels were explored in patients with polycystic ovary syndrome (PCOS) undergoing in vitro fertilization/intracytoplasmic sperm injection.  Methods: This case-control study involved 20 infertile women with PCOS and 20 control women diagnosed as poor ovarian responders stimulated with a GnRH antagonist. Blood samples were taken during ovum pick-up and follicular fluids (FF) were obtained from a dominant follicle from the subjects. Samples were analyzed by using ELISA. Statistical analysis was performed with SPSS version 20. Data are expressed as means ± standard deviation (SD). Results: Blood NUCB2/Nesfatin-1 levels in PCOS were significantly lower (p=0.011) while the NUCB2/Nesfatin-1 levels of FF in poor ovarian response (POR) were higher, but not statistically significant.  Insulin, total testosterone, fasting glucose, homeostasis model assessment, and insulin resistance index in women with POR decreased when compared with PCOS. Blood NUCB2/Nesfatin-1 levels were significantly higher than FF NUCB2/Nesfatin-1 levels in both groups (p<0.001). Moreover, a positive correlation was detected between blood NUCB2/Nesfatin-1 and testosterone (p=0.602, r=0.304), HOMA-IR (p=0.252, r=0.384), BMI (p=0.880, r=0.44) in PCOS, but it was not significant.  Conclusion: NUCB2/Nesfatin-1 levels might be important in follicular growth in PCOS subjects undergoing IVF/ICSI with an antagonist protocol and NUCB2/Nesfatin-1 level could reliably help to predict poor ovarian response.