J Reprod Infert

arij001

Journal of Reproduction & Infertility

2228-5482 2251-676X

Avicenna Research Institute

jri669

Effect of Acetylcholinesterase and Butyrylcholinesterase on Intrauterine Insemination, Contribution to Inflammations, Oxidative Stress and Antioxidant Status; A Preliminary Report

Haghnazari

Lida

Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Vaisi-Raygani

Asad

Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, IranMolecular Diagnostic Research Center, Kermanshah University of Medical Sciences, Kermanshah, IranDepartment of Clinical Biochemistry, Kermanshah University of Medical Sciences, Kermanshah, Iran

Keshvarzi

Farahnaz

Department of Obstetrics and Gynecology, Kermanshah University of Medical Sciences, Kermanshah, Iran

Ferdowsi

Farivar

Department of Obstetrics and Gynecology, Kermanshah University of Medical Sciences, Kermanshah, Iran

Goodarzi

Massoud

Molecular Diagnostic Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Rahimi

Zohreh

Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Baniamerian

Hossin

Department of Clinical Biochemistry, Kermanshah University of Medical Sciences, Kermanshah, Iran

Tavilani

Haidar

Department of Clinical Biochemistry, Hamadan University of Medical Sciences, Hamadan, Iran

Vaisi-Raygani

Hadis

Molecular Diagnostic Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Vaisi-Raygani

Hessam

Molecular Diagnostic Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Pourmotabbed

Tayehbeh

Department of Microbiology, Immunology and Biochemistry, University of Tennessee, Health Science Center, Tennessee, USA

17 3 157 163 1 8 2015 16 11 2015

Background: Oxidative stress affects women fertility and influences on the sperm quality by alterating activities of cholinesterases, a molecular marker of stress-related infertility. The aim of the present study was to investigate the role of acetylcholinesterase (AChE), butyrylcholinesterase (BuChE) activities and phenotypes in patients with unexplained infertility (idiopathic). It’s possible association with inflammation marker C-reactive protein (CRP) and other oxidative stress markers, i.e. before and after intra uterine insemination (IUI). Methods: In this study, blood samples of 60 patients with unexplained infertility were collected the day before and 24 hr after IUI (between 8 AM and 9 AM after the overnight fasting) and activities of BuChE, AChE, catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GpX) and serum levels of thiol proteins (TP), C-reactive protein (CRP), total antioxidant capacity (TAC) were measured. Statistical significance was assumed at p<0.05. Results: Before IUI, there was a significant (p=0.048) positive correlation between BuChE activity and plasma TAC and a significant difference in the CAT activity between various BuChE (UU and non-UU) phenotypes. However, after IUI, a significant negative correlation between the AChE activity and BuChE activity was found (p=0.045) and the level of RBC AChE activity was significantly reduced (382.4±163.19 vs. 586.7±384 IU/grHb, p=0.025). Meanwhile, after IUI, the activities of SOD (1568±847.5 IU/grHb vs. 1126±229.3, p=0.031) and CAT (310±53.4 IU/grHb vs. 338±73, p=0.025) were increased. Conclusion: This study suggests that decline in cholinesterases activities may be responsible for stimulation of oxidative stress and inflammation and reduction in fertility rates by IUI.