J Reprod Infert

arij001

Journal of Reproduction & Infertility

2228-5482 2251-676X

Avicenna Research Institute

jri80077

The Anti-Inflammatory Effect of Octyl Gallate Through Inhibition of Nuclear Factor-κB (NF-κB) Pathway in Rat Endometriosis Model

Bustami

Arleni

Integrated Laboratory of Medical Faculty, Universitas Indonesia, Jakarta, Indonesia

Lestari

Wahyu

Master Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia

Hayuningrum

Cicilia

Departement of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia

Wibowo

Heri

Integrated Laboratory of Medical Faculty, Universitas Indonesia, Jakarta, IndonesiaDepartement of Parasitology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia

Wuyung

Puspita

Departement of Pathology Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia

Natadisastra

R. Muharam

Departement of Obstetrics and Gynecology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia

21 3 169 176 15 10 2019 10 3 2020

Background: Endometriosis is a chronic inflammatory condition associated with an increased risk of epithelial ovarian cancer. Our previous studies found that the anti-inflammatory effect of octyl gallate in endometriosis cell culture was more effective than gallic acid and heptyl gallate. This study aimed to analyze the anti-inflammatory effect of octyl gallate in rat endometriosis model. Methods: Thirty female Wistar rats were randomly divided into three groups. Group I was the sham-operated group, group II was the surgically-induced group, whereas group III was the surgically-induced group and each rat was administered with 20 mg of octyl gallate dissolved in 1 ml Na-CMC via oral gavage once a day for 30 days. When all rats were euthanized, the endometrial tissue from group I and last two groups were collected for further analysis. TNF-α levels were measured using Luminex, while non-phosphorylated NF-κB and COX-2 levels were analyzed using ELISA.  Results: The average of non-phosphorylated NF-κB levels in group III (4.970±0.971 pg/mgP) was significantly higher than group II (3.97±0.656 pg/mgP). Moreover, the proportion of rats with the high level of non-phosphorylated NF-κB in group III was 45.6% higher than group II (p<0.05). The proportion of rats with the high level of COX-2 in group III was 22.3% lower than group II (p<0.05). However, there was no significant difference in TNF-α levels between all groups.  Conclusion: The anti-inflammatory effect of octyl gallate may has effects in NF-κB activation and reduction of COX-2 levels in rat endometriosis model.