AbooTaleb Saremi Corresponding Author
1- Department of Obstet . and Gynecol Faculty of Medicine, Islamic Azad University, Tehran, Iran
2- Sarem Medical Center, Tehran, Iran
Mahnaz Azarnia
- Department of Biology, Faculty of Science, Tarbiat Moalem University, Tehran, Iran
Mojtaba Dashtizad
- Research & Development Unit, Sarem Medical Center, Tehran, Iran

Received: 7/1/2003 Accepted: 7/1/2003 - Publisher : Avicenna Research Institute

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Having baby is a desire which plays a major role in everybody’s life. Infertility as unsuccess in achieving this is an annoying matter, affecting approximately 20% of couples. However, this fact should not be ignored that there is still many unknown problems related to infertility. Polycystic ovarian syndrom (PCOS) is one of these Problems. In our study, recovery of immature oocytes followed by in vitro maturation (IVM) of these oocytes was developed as a new method for the first time in Iran for treatment of patients with infertility due to PCOS. The purpose of this study was to reduce the side effects of the currently used treatments such as ovarian hyperstimulation syndrome, ascites, circulation dysfunction, renal dysfunction, thrombosis, multiple pregnancy ,…, as well as their expensive cost. Immature oocytes were collected by transvaginal ultrasound and then transferred to maturation medium for culture. After 24 and 48h of incubation, mature oocytes were transferred to endometerium. Spermatozoa for ICSI were prepared by Swim up, and injected to the mature oocyte with micromanipulator system. A total of 52 immature oocytes were retrieved, all of which matured after incubation(100%); 49 mature oocytes were injected and 35 were cleaved (71%). Satisfactory results of IVM have been published, thus due to its advantages and suprising reduction of the medical cost, it could be as a miracle in curing infertility.

Keywords: Maturation, Oocyte, In vitro maturation, Without induction of ovulation

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  1. اسفندياري‌نويد.بررسي‌تاثير برخي از عوامل ايمونولوژيك و ناباروري با علت نامشخص. پايان نامه دكتراي تخصصي، دانشگاه علوم پزشكي و خدمات بهداشتي، درماني تهران، 73-1372.
  2. Frank S. The Polycystic Ovary Syndrome. N Engl J Med.1995;333:853-61.
  3. Futterweit W. Pathophysiology of Polycystic Ovarian Syndrome. Redmond G.P(Editor)., Androgenic Disorders. Raven Press, New York. 1995;77-166.
  4. Balen A.H. The phatogenesis of Polycystic Ovary Syndrome.Lancet.1999;354:966-7.
  5. Swanson M., Sauerbrie E.E., Cooperberg P.L. Medical implications of ultrasonically detec polycyctic ovaries. J Clin Ultrasound.1981;9:219-22.
  6. Adams J., Frank S., Polson D.W., Mason H.D., Abdulwahid N., Jacobs H.S.Multifollicular ovaries: clinical and endocrine features and response to pulsatile gonadotrophin releasing hormone. Lancet. 1985;1375-8.
  7. Pincus G., Enzmann E.V. The comparative behaviour of mammalian eggs in vivo and in vitro. J Exp Med. 1935;65:665-75.
  8. Edwards R.G., Bavister B.C., Steptoe P.C. Early stage of fertifization In Vitro of Human oocytes matured In vitro. Nature.1969;221:632-5.
  9. Jacobs H.S. Polycystic Ovaries and Polycystic Ovary Syndrome. Gyn Endocrinol.1987;1:113-31.
  10. Yen S.S.C. The Polycystic Ovary Syndrome. Clin Endocrinol.1980;12:177-86.
  11. Chian R.C., Gulekli B.Pregnancy and delivery after cryopreservation of zygotes produced by in-vitro matured oocytes retrieved from a woman with Polycystic Ovarian Syndrome. Hum Reprod.2001;16,8,1700-2001.
  12. Tim J. In vitro maturation and fertilization of oocytes from unstimulated normal ovaries,and women with polycystic ovary syndrome. Fertil Steril.2001;76,5:936-41.
  13. Trounson A., W., C., In vitro maturation and the fertilization and developmental competence of oocytes recovered from untreated polycystic ovarian patients. Fertil Steril. 1994;62; (2):353-62.
  14. Ben- ze’ev A., Amsterdam A.Regulation of cytoskeletal proteins invo lved in cell contact formation during differentiation of granulosa cells on extracellular matrix.Proc Natl Acad Sci USA.1998;83:2894-8.
  15. Kwang Y., Cha Se., Han Y. Pregnancies and deliveries after in vitro maturation culture followed by in vitro fertilization and embryo transfer without stimulation in women with Polycystic Ovary Syndrome. Fertil Steril.2000;73(5):978-83.
  16. Cortvrindt R., Smitz J. In vitro follicle growth: Achievements in mammalian species. Reprod Dom Anim.2001;36: 3-9.
  17. Liu j., Katze., Garcia J.E., et al. Successful In Vitro maturation of human oocytes not exposed to human chorionic gonadotropin during ovulation induction, resulting in Pregnancy. Fertil Steril. 1997;67:566-8.
  18. Barnes F.L., Kausche A., Tiglias J., et al. Production of embryos from in vitro matured primary human Oocyte. Fertil Steril.1996;65: 1151-6.
  19. Willis D.S., Mason H.D. Premature response to LH of granulose cells from anovlatory women with PCOS: Relevance to mechanism of anovulation. J Clin Endocrinol Metab.1998;83:3984-91.
  20. Almahboobi G., Anderiesz C. Functional integrity of granulose cells from polycystic ovaries. Clin Endocrinol.1996;44:571-80.
  21. Gardner D.K., Lane M., Rodriguez-Martinez H. Fetal development after transfer is increased by replacing protein with the glycosaminoglycan hyaluronate for embryo culture. Hum Reprod. 1997;12:0-215.
  22. Regan L., Owen E.J. Hypersecretion of luteinising hormone, infertility and miscarriage. Lancet.1990;336:1141-4.
  23. Hardy K., Wright C.S. In vitro maturation of oocytes. Brith Med Bull. 2000;56(3):588-602.
  24. Russell J.B., Knezevich K.M., Fabian K.F., Dickson J.A. Unstimulated immature oocyte retrieval: early versus midfollicular endometrial Priming. Fertil Steril.1997;(67):616-20.
  25. Green D.P.L. Three- dimensional structure of the zona Pellucida.Rev Reprod.1997;2:147-56.
  26. Schramm R.D., Baoister B.D. Follicle- Stimulating hormone priming of rhesus monkeys enhances meiotic and developmental competerce of oocytes matured invitro. Biol Reprod.1994;5151:904-12.
  27. Cobo Ana C., Requena A. Maturation in vitro of human oocytes from unstimulated cycles: Selection of the optimal day for ovum retrieval based on follicular size. Hum Reprod. 1999; 14(7):18 64-8.
  28. Wiley L.M., yanami S., Van Muyden D. Effect of potassium concentration, type of protein Supplement and embryo density on mouse preimplantation development in vitro. Fertil steril.1986;45:111-19.
  29. Lane M., Gardner D.K. Effect of incubation volume and embryo density on the development and viability of Preimplantation mouse embryos in vitro. Hum Reprod.1992;7:558-62.
  30. Ahern T., Gardner D.K. Culturing bovine embryos in groups Stimulates blastocyst development and cell allocation to the inner cell mass.Theriogenology.1998;49:194.
  31. Gardner D.K., Lane M.W., Lane M. Development of the inner cell mass in mouse blastocysts is stimulated by reducing the embryo: incubation volume ratio. Hum Reprod.1997;12: 132.
  32. Cumming J.M., Breen T.M., Harrison K.L., et al. A formula for scoring human embryo growh rates in invitro fertilization: its value in predicting pregnancy and in comparison with visual estimates of embryo quality.J In vitro Fertil Embryo Transf.1986;3:284-95.
  33. Bolton V.N., Hawes S.M., Taylor C.T., Parsons J.H. Development of spare human preimplatntation embryos in vitro and analysis of the correlations among gross morphology, cleavage rates and development to the blastocyst. J In vitro Fertil Embryo Transf.1989;6:30-5.
  34. Trounson A.O., wood C., kausche A. In vitro maturation and fertilization and developmental competence of oocytes recovered from untreated- polycystic ovarian Patients. Fertil Steril. 1994;62: 353-62.
  35. Racowsky C., kaufman M.L. Nuclear degeneration and meiotic aberrations observed in human oocytes matured in vitro: analysis by light microscopy. Fertil Steril.1992;58:750-5.
  36. Gras L., Mcbain J., Trounson A.o., Kola I. The incidence of chromosomal aneuploidy in stimulated and unstimulated (natural) uninseminated human oocytes.Hum Reprod. 1992;7:1396-401.
  37. Delhanty J.D., Harper J.C., Handyside A.H., Winston R.M. Multicolour fish detects frequent chromosomal mosaicisms and chaotic division in normal Preimplantation embryos from fertile patients. Hum Genet.1997;99:755-60.


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