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Allostimulatory efficiency of splenic dendritic cells from pregnant and non pregnant mice

Vol. 5, Issue 1, / January-March 2004
(pages 5-13)

Jaleh Shojaeian
- Immunology Department, Faculty of Medical Science, Tarbiat Modarres University, Tehran, Iran
Seyyed Mohammad Moazzeni Corresponding Author
- Immunology Department, Faculty of Medical Science, Tarbiat Modarres University, Tehran, Iran
Amir Hassan Zarnani
1- Immunology Department, Faculty of Medical Science, Tarbiat Modarres University, Tehran, Iran
2- Nanobiotechnology Research Center, Avicenna Research Institute (ACECR), Tehran, Iran

Received: 1/1/2004 Accepted: 1/1/2004 - Publisher : Avicenna Research Institute

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Abstract

Introduction: Mammalian reproduction looks like an immunological paradox, because fetal alloantigens encoded by father genes should induce cell mediated immune responses leading to fetal loss. Maternal immune system, in addition to local modulation, undergoes systemic modulations during pregnancy. Dendritic cells (DCs), as professional antigen presenting cells, play a key role in initiation and control of immune response and it seems that functional changes in these cells during pregnancy may contribute to the systemic immune tolerance. To address this issue, in this study we isolated and purified DCs from pregnant mice and evaluated their stimulatory potential to induce proliferative response of allogenic T cells in unidirectional mixed leukocyte reaction (MLR). Materials and Methods: Following collagenase digestion of splenic tissue, using density gradient centrifugation (13% Nycodenz) and adherence properties of DCs to the bottom of tissue culture dish, 7×105 DCs were isolated from each spleen with more than 95 percent purity. Allogenic T cells were isolated by nylon ‌wool column, using their non-adhesive character to nylon wool. After radiation, isolated dendritic cells from pregnant and non-pregnant Balb/c mice were used in mixed leukocyte culture with C57BL/6 mice T lymphocytes. T lymphocyte proliferation was measured after 72 hours by 3H- thymidine incorporation. Results: 7 105 dendritic cells with the purity of >95% were isolated from each spleen. Also the yield of T- lymphocyte form Inguinal and Brachial lymph nodes was about 3-5105 with the purity of %85-90. The results showed that there is no statistical difference between stimulatory potential of DCs form pregnant (cpm=33000) and non- pregnant (cpm=35000) mice in induction of allogenic T-Cell proliferation. Conclusion: These findings can result from low concentration of immune suppressor factors in circulatory system of pregnant mice or due to separation of dendritic cells from pregnancy microenvironment and their maturity in vitro in the absence of the immune suppressor factors.


Keywords: Dendritic cell, Spleen, Pregnancy, Mixed leukocyte culture, Allogenic response


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