Jaleh Shojaeian
- Immunology Department, Faculty of Medical Science, Tarbiat Modarres University, Tehran, Iran
Seyyed Mohammad Moazzeni Corresponding Author
- Immunology Department, Faculty of Medical Science, Tarbiat Modarres University, Tehran, Iran
Amir Hassan Zarnani
1- Immunology Department, Faculty of Medical Science, Tarbiat Modarres University, Tehran, Iran
2- Nanobiotechnology Research Center, Avicenna Research Institute (ACECR), Tehran, Iran

Received: 1/1/2004 Accepted: 1/1/2004 - Publisher : Avicenna Research Institute

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Introduction: Mammalian reproduction looks like an immunological paradox, because fetal alloantigens encoded by father genes should induce cell mediated immune responses leading to fetal loss. Maternal immune system, in addition to local modulation, undergoes systemic modulations during pregnancy. Dendritic cells (DCs), as professional antigen presenting cells, play a key role in initiation and control of immune response and it seems that functional changes in these cells during pregnancy may contribute to the systemic immune tolerance. To address this issue, in this study we isolated and purified DCs from pregnant mice and evaluated their stimulatory potential to induce proliferative response of allogenic T cells in unidirectional mixed leukocyte reaction (MLR). Materials and Methods: Following collagenase digestion of splenic tissue, using density gradient centrifugation (13% Nycodenz) and adherence properties of DCs to the bottom of tissue culture dish, 7×105 DCs were isolated from each spleen with more than 95 percent purity. Allogenic T cells were isolated by nylon ‌wool column, using their non-adhesive character to nylon wool. After radiation, isolated dendritic cells from pregnant and non-pregnant Balb/c mice were used in mixed leukocyte culture with C57BL/6 mice T lymphocytes. T lymphocyte proliferation was measured after 72 hours by 3H- thymidine incorporation. Results: 7 105 dendritic cells with the purity of >95% were isolated from each spleen. Also the yield of T- lymphocyte form Inguinal and Brachial lymph nodes was about 3-5105 with the purity of %85-90. The results showed that there is no statistical difference between stimulatory potential of DCs form pregnant (cpm=33000) and non- pregnant (cpm=35000) mice in induction of allogenic T-Cell proliferation. Conclusion: These findings can result from low concentration of immune suppressor factors in circulatory system of pregnant mice or due to separation of dendritic cells from pregnancy microenvironment and their maturity in vitro in the absence of the immune suppressor factors.

Keywords: Dendritic cell, Spleen, Pregnancy, Mixed leukocyte culture, Allogenic response

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  1. Levin R.W., Aoki K., Azuma T., Yagami Y., Okada H. Human pregnancy serum suppresses the proliferative response of lymphcytes to outo-logous PHA-activated T lymphoblasts. Am J Reprod Immunol.1996;35:63-69.
  2. Thellin O., Coumans B., Zorzi W., Igout A., Heinen E. Tolerance to the foeto- placental graft: ten ways to support a child for nine months. Currt Opin Immunol.2000;12:731-737.
  3. Heyborn K., Silver R. Immunology of post-implantation Pregnancy: In Reproductive immunology. Bronson R.(Editor),1996.
  4. مصفا نريمان، زرناني اميرحسن، زهيرمحمد حسن. ايمونوبيولوژي حاملگي طبيعي. چاپ اول، انتشارات دانشگاه علوم پزشكي شهيدبهشتي(1382)، فصل ششم، صفحات: 181-164.
  5. Darmochwal- Kolarz D., Rolinski J., Tabarkiewicz J., Leszczynska- Goyzelak B., Buczkowski J., Wojas K., Oleszczuk J. Blood myeloid and lymphoid dendritic cells are stable during the menstrual cycle but dificient during mid-gestation. J Reprod Immunol.2003;59:193-203.
  6. Luppi P. How immune mechanisms are affected by pregnancy. Vaccine.2003;21: 3352-3357.
  7. Sacks G., Sargent I., Redman Ch. An innate view of human pregnancy. Immunol Today. 1999;20:114-118.
  8. Vermec D., Zorbas M., Scollay R., Saunders D. J., Ardavin C.F., Wu L., Shortman K. The surface phenotype of dendritic cells purified from mouse thymus and spleen, Investigation of the CD8 expression by a subpopulation of dendritic cells. J Exp Med.1992;176:47-58.
  9. Litvin D., Rosenstreich D. Separation of lymphoid cells on nylon wool columns. Meth Enzymol.1984;108:298-302.
  10. Crowley M.T., Inaba K., Witmer-Pack M.D., Gezelter S., Steinman R.M. Use of the fluorescence activated cell sorter to enrich dendritic cells from mouse spleen. J Immunol Meth.1990;133:55-66.
  11. Matthiesen L., Berg G., Ernerudh J., Hakansson L. Lymphocyte subsets and mitogen stimulation of blood lymphocytes in normal pregnancy.Am J Reprod Immunol.1996;35:70-79.
  12. Yokoyama W.M. The mother-child union: The case of missing- self and protection of the fetus. Proc Acad Sci USA.1997;94:5998-6000.
  13. Mellor A.L., Munn D.H. Immunology at the maternal-fetal interface: lessons for T cell tolerance and suppression. Annu Rev Immunol. 2000;18:367-391.
  14. Sridama V., Pacini F., Yang S.L. Decreased levels of T helper cells: a possible cause of immunodeficiency in normal pregnancy. N Engl J Med.1982;307:365-367.
  15. Moore M.P., Carter N.P., Redman C.W. Lymphocyte subsets in normal and pre- eclamptic pregnancies. Br J Obstet Gynaecol.1983;90:326-331.
  16. Tallon D.F., Corcoran D.J., Odwyer E.M., Greally J F. Circulating lymphocyte subpopula-tion in pregnancy, a longitudial study. J Immunol. 1984;132:1784-1787.
  17. Gonik B., Loo L.S., West S. Natural killer cell cytotoxicity and antibody-dependent cellular cyto-toxcicity to herpes simplex virus-infected cells in human pregnancy. Am J Reprod Immunol Microbiol.1997;13:23-26.
  18. Kovar I.Z., Riches P.G. C3 and C4 comple-ment components and acute phase proteins in late pregnancy and parturition. J Clin Pathol.1988;41: 650-652.
  19. Kang J.A., McBey B.A., Angkachatchai V., Croy B.A., Beaman K.D. Expression of Tj6 during pregnancy. Am J Reprod Immunol. 1997;38:183-187.
  20. Amtzen K.J., Liabakk N.B., Jacobsen G., Espevik T., Austgulen R. Soluble tumor necrosis factor receptor in serum and urine throghout normal pregnancy and at delivery. Am J Reprod Immunol.1995;34:163-169.
  21. Aari A., Kristofferson E.K., Jensen T.S., Ulvested E., Matre R. Suppressive effect on lymphoproliferation in vitro by soluble annexin II released from isolated placental membranes. Am J Reprod Immunol.1997;38:313-319.
  22. Khair-el-din T.A., Sicher S.C., Vazquez M.A., Lu C.Y. Inhibition of macrophage nitric-oxide production and Ia- expression by docosahexaenoic acid, a constituent of fetal and neonatal serum. Am J Reprod Immunol.1996;36: 1-10.
  23. Morton H.Early pregnancy factor: An extra-cellular chaperonin 10 homologue. Immunol Cell Biol.1998;76:483-496.
  24. Kelemen K., Paldi A., Tinneberg H., Torok A., Szekeres- Bartho J. Early recognition of pregnancy by the maternal immune system. Am J Reprod Immunol.1998;39:351-355.
  25. Invernizzi P., Battezzati P.M., Podda M., Simoni G. Presence of fetal DNA in maternal plasma decades after pregnancy: further comments. Hum Genet.2002;110(6):587-91.
  26. Thellin O., Heinen E. Pregnancy and the immune system: between tolerance and rejection. Toxical.2003;185(3):179-84.
  27. Banchereau J., Briere F., Caux Ch., Davoust J., Lebecque S., Liu Y., Pulendran B., Palucka K. Immunobiology of dendritic cells. Annu Rev Immunol.2000;18:767-811.
  28. Gad M., Claesson M.H., Pedersen A.E. Dendritic cells in peripheral tolerance and immunity. APMIS. 2003;111(7-8):766-75.
  29. Yoshimura T., Inaba M., Sugiura K., Nakajima T., Ito T., Nakamura K., Kanzaki H., Ikehara S. Analysis of dendritic cell subsets in pregnancy. Am J Reprod Immunol.2003;50:137-145.
  30. Le Friec G., Laupeze B., Fardel O., Sebti Y., Pangault C., Guilloux V., Beauplet A., Fauchet R., Amiot L. Soluble HLA-G inhibits human dendritic cell- triggered allogeneic T- cell proliferation without altering dendritic differentia-tion and maturation processes. Hum Immunol. 2003;64:752-61.
  31. Steinbrink K., Wolfl M., Jonuleit H., Knop J., Enk A.H. Induction of tolerance by IL-10-treated dendritic cells. J Immunol.1997;159:4772-80.
  32. Kalinski P., Schuitemaker J.H., Hilkens C.M., Kapsenberg M.L. Prostaglandin E2 induces the final maturation of IL-12-deficient CD1a+CD83+ dendritic cells: the levels of IL-12 are determined during the final dendritic cell maturation and are resistant to further modulation. J Immunol.1998; 161:2804-9.


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