Introduction
In vitro maturation (IVM) has been established in various species since 1935 (1). In the early 1960s, Robert Edwards and his colleagues made remarkable efforts to explore IVM as an alternative strategy for treating human infertility (2), with successful applications beginning in 1991 (3, 4). IVM is defined as the retrieval of immature oocytes from 2-10 mm follicles from unstimulated or minimally stimulated ovaries, followed by in vitro culture until they reach metaphase II (MII) stage (5). Despite significant advances over the years, human oocyte maturation remains challenging due to asynchronous nuclear and cytoplasmic maturation, limiting its efficiency in achieving successful pregnancy compared to conventional IVF. The key to IVM success lies in preserving the integrity of the bidirectional communication structure between cumulus cells and oocytes, particularly through transzonal projections (TZPs) and gap junctions, which play a critical role in oocyte maturation (5). Removal of cumulus-oocyte complex (COC) from the ovary disrupts the flow of meiotic inhibitors, the so-called cyclic adenosine monophosphate (cAMP) and/or cyclic guanosine monophosphate (cGMP) between cumulus cells and oocytes. As a consequence, phosphodiesterase 3A enzymes are activated, leading to the phosphorylation of connexin proteins within gap junctions. In addition, lower cGMP levels result in the degradation of intra-oocyte cAMP by phosphodiesterase, causing the premature resumption of meiosis.
The breakthrough discovery of a c-type natriuretic peptide (CNP) molecule in regulating cGMP in mouse oocytes (6) and preserving the integrity of the bidirectional communication structure, which in turn controls the activation of phosphodiesterase enzymes and prevents germinal vesicle breakdown, has significant potential to improve the clinical practice of IVM (5). The understanding that CNP/cGMP acts as upstream modulator for cAMP led to the introduction of a biphasic or pre-IVM culture approach in 2015, which has become an established routine IVM known as capacitation IVM (CAPA-IVM) in 2020 (5). This approach has demonstrated pregnancy outcomes that are comparable or superior to those of conventional stimulation in selected patients (7). Briefly, the method consists of a two-step culture of COCs. In step one, culture medium contains CNP as a meiosis inhibitor, prevent-ing precocious meiosis resumption and allowing oocytes to continue mRNA transcription activity, which is crucial in supporting developmental competence. In step two, the culture medium enables the oocyte to resume meiosis.
The first successful conversion of human germinal vesicle (GV) oocytes to mature MII oocytes using the CAPA-IVM system in Indonesia is reported in this paper. COCs were successfully retrieved without ovarian stimulation or the administration of an oocyte maturation trigger.
Case Presentation
A woman aged 37 years presented to our IVF clinic with a 14-year history of primary infertility and irregular menstrual cycles. This patient had a history of poor ovarian response to gonadotropin stimulation across three prior IVF cycles performed at other IVF clinics. Records were available only for the most recent of the three cycles, which was performed in 2017 (Table 1). In her last ovarian stimulation cycle, controlled ovarian stimulation was initiated using a starting gonadotropin dose of 300 IU daily for seven days. After seven injections, the clinician determined that the patient had responded poorly to gonadotropin stimulation; therefore, the cycle was discontinued, and the patient was advised to re-turn to the clinic on day 2/3 of her next menstrual period. However, the patient did not return for further treatment at that time. Across all three stimulation cycles, follicular development was inadequate, leading to cancellation of ovum pick-up in each attempt.
In March 2024, as a final attempt to conceive, the patient visited our clinic seeking another possible treatment. Her BMI was within the normal range (20.17 kg/m2). Ovarian reserve assessment revealed an anti-Mullerian hormone (AMH) level of 4 ng/ml, and a basal antral follicle count (AFC) of 20. Baseline hormonal evaluation revealed an estradiol level of 60.29 pg/ml, a basal follicle-stimulating hormone (FSH) level of 19 mIU/ml, a basal luteinizing hormone (LH) level of 13.48 mIU/ml, and a progesterone level of 0.11 ng/ml.
After clinical evaluation, the IVM approach was offered by the clinician in August 2024, considering the patient’s suspected gonadotropin resistance. Following approval from the advisory board of the clinic and comprehensive counseling regarding the procedure, potential benefits, and associated risks, the patient consented to undergo ovum pick-up retrieval without ovarian stimulation. Transvaginal ovarian follicle puncture was performed on day 11 of the menstrual cycle. Under mild sedation, eight follicles measuring 2-7 mm in diameter, with an average size of 4 mm, were seen under ultrasound. A 20-gauge single-lumen IVM needle (Kitazato, Japan) was used to retrieve five immature oocytes. GMOPS medium (Vitrolife, Sweden), supplemented with 50 nM CNP (Sigma-Aldrich, USA; Lot No. SLCF0284), 20 nM estradiol (Sigma-Aldrich, USA; Cat. No. E1024, Lot No. SLCQ6890), and 10 mg/ml human serum albumin (HSA; Vitrolife, Sweden), was used as the aspiration medium. The aspiration medium was supple-mented with 50 nM CNP to maintain meiotic arrest in the COCs immediately following aspiration and throughout the collection and handling procedures (Figure 1). As estradiol supports the CNP-NPR2 pathway, adding 20 nM estradiol would be beneficial for preserving the physiological follicular environment during oocyte retrieval (8). To optimize the search process, follicular fluid was filtered through a cell strainer.
The CAPA-IVM medium was prepared according to the protocol described by Sanchez et al. (9). Briefly, the base medium (Medicult IVM System; Origio, Denmark) was supplemented with 25 nM CNP, 10 mg/ml human serum albumin (HSA), 1 mIU/ml recombinant follicle-stimulating hormone (rFSH; Gonalf®, Merck Serono, Switzerland), 10 nM estradiol, and 5 ng/ml insulin (Sigma-Aldrich, USA; Cat. No. I9278). The concentration of 25 nM CNP used in the CAPA medium was selected based on previous findings indicating that it is optimal for mimicking the physiological action of CNP. The obtained COCs were incubated in 500 µl of CAPA medium covered with mineral oil at 37oC and 6% CO2 for 24 hr. Following the pre-IVM culture period, the COCs were washed and transferred to maturation medium (Medicult IVM System; Origio, Denmark) supplemented with 100 ng/ml recombinant human amphiregulin (R&D Systems, USA), 5 ng/ml insulin (Sigma-Aldrich, USA), 10 nM estradiol, 10 mg/ml HSA, and 100 mIU/ml recombinant follicle-stimulating hormone (rFSH; Gonal-f®, Merck Serono, Switzerland). Oocyte maturation was assessed after 30 hr of culture, and all five COCs reached the MII stage, corresponding to a maturation rate of 100% (Figure 2). Detailed evaluation using the OCTAX PolarAIDE™ system confirmed the presence of a meiotic spindle in all mature oocytes (Figure 3).
Ethics approval
Since no intervention was performed in this study, ethical approval is not required. Therefore, investigators may proceed with their publication, provided they maintain the confidentiality and anonymity of the study subject. Written informed consent from the patient was retrieved.
Discussion
In this case, fertilization was unfortunately not performed immediately after IVM because the male partner was unable to provide a semen sample following completion of the IVM procedure. Consequently, all mature oocytes were cryopreserved. However, our results support previous findings indicating that the CAPA-IVM approach improves maturation of oocytes derived from small antral follicles. Sanchez et al. (9) further reported favorable embryological outcomes following CAPA-IVM. Likewise, in a study involving 80 women diagnosed with PCOS, Vuong et al. (10) reported that CAPA-IVM achieved a significantly higher oocyte maturation rate than conventional IVM (63.6% versus 49%, p<0.001 ). as this was our first capa-ivm case, predefined eligibility criteria had not been established.
Despite concerns regarding the clinical application of CAPA-IVM, including the need to identify the patients most likely to benefit from the procedure and the relatively high attrition rate during embryo development, the potential benefits of CAPA-IVM remain substantial. In particular, this approach may reduce the cost of treatment by eliminating the need for ovarian stimulation and monitoring, which is a major expense in Indonesia. Therefore, for selected patients, especially those for whom treatment cost is a major concern, successful oocyte maturation through CAPA-IVM may offer a promising alternative. The potential reduction in treatment costs could significantly increase the acces-sibility and adoption of IVF in Indonesia, which currently lags behind in the number of IVF cycles despite its large population and a GDP per capita similar to that of countries such as Vietnam. Other developed countries, such as Belgium and Australia, have yet to widely adopt CAPA-IVM due to sub-sidized medication costs and historical concerns. A shift towards drug-free IVF could offer substantial cost savings and encourage broader utilization, particularly in contexts where drug costs are a significant barrier.
Conclusion
This first reported case of CAPA-IVM in Indonesia demonstrates its successful implementation without ovarian stimulation. All retrieved immature oocytes reached the MII stage, as confirmed by polar body extrusion and spindle formation. These findings suggest that CAPA-IVM may offer a simpler and more affordable IVF approach, although further studies are warranted to confirm its clinical effectiveness.
Conflict of Interest
None.