Leila Roshangar
- Histology Department, Faculty of Medicine, Tabriz Medical Sciences University, Tabriz, Iran
Jafar Soleimanirad Corresponding Author
- Drug applied research center, Tabriz University of Medical ٍSciences, Tabriz, Iran

Received: 10/1/2004 Accepted: 10/1/2004 - Publisher : Avicenna Research Institute

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Introduction: Humans are continuously exposed to harmful environmental factors, such as electromagnetic fields (EMF) produced by home appliances, diagnostic tools and industrial instruments. The adverse effects of electromagnetic field on biological systems has been shown by epidemiologic and experimental studies. However, the results about the exact mechanism of its actions and its effects on different organs are not conclusive. The present study is designed to investigate morphological effects of EMF on ovarian follicles, at cellular level, using electron microscope. Materials and Methods: For this purpose, 30 adult female wistar rats were used (15 as control and 15 as experimental group). The rats in experimental group were exposed to 3 milli Tesla EMF for 4h/day for 4 months. After the experimental period, the ovaries eblated from both experimental and control groups, after proper fixation and processing, the thin sections were studied by transmission electron microscopy (TEM). Morphometric studies were carried out on electron micrographs using measurements and counting techniques. The data were analysed using students T- test. Results: Electron micrographs revealed that in EMF exposed group, in comparison to control group, the oocytes had shrunk and the thickness of zona pellucida and the number of penetrated microvilli were significantly (P<0.001 ) reduced (4.470.42 vs. 3.240.25 as 9.80.56 vs. 4.130.83 respectively). in the cytoplasm of oocytes from the experimental group the lamella were condensed. the granulosa cells from the emf-exposed rats showed nuclear condensation and chromatin margination and several vacuoles appeared in their cytoplasm. the other morphological changes inclued; disappearance of mitochondrial cristaes, appearance of apoptotic signs such as separation from neighboring cells and appearance of apoptotic bodies. these changes were associated with an increase of macrophages. conclusion: our results suggest that emf-exposure inhibits follicular growth and oocyte maturation by affecting cellular function, membrane changes and inducing apoptosis. it is concluded that emf exposure, by the above mechanisms, could interfere with normal process of oogenesis and lead to subfertility.< pan>

Keywords: Electromagnetic field, Transmission electron microscope (TEM), Apoptosis, Rat, Ovary, Follicle, Granulosa cell

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  1. Michael H., Repacholi B., Greenebaum B. Inte-raction of static and extremely low frequency electric and magnetic fields with living systems: Health effects and research needs. Bioelectromag-netics.1999;20:133-160.
  2. Riminesi C., AndreucceHi D., Fossi R., Pezzati M. ELF magnetic field exposure in a neonatal intensive care unit. Bioelectromagnetics.2004;25:481-491.
  3. Brent R.L., Gordan W.E., Bennett W.R., Beck-man D.A. Reproductive and teratogenic effects of electromagnetic field. Reprod Toxicol.1993;7: 535-580.
  4. Barnes F.S. Some engineering models for inter-actions of electric and magnetic fields with biolo-gical systems. Bioelectromagnetics.1992;Sup1:67-85.
  5. Levin M. Bioelectromagnetics in morphogene-sis. Bioelectromagnetics.2003; 24:295-315.
  6. Cheronff N., Rogers J.M., Kavet R. A review of the literature on potential reproductive and developpmental toxicity of electric and magnetic fields. Toxicology.1992 ;74: 91-126.
  7. Ahmed E., Mohd ALI A ., Homa D.Long-term exposure of male and female mice to 50 HZ magnetic field: effects on fertility. Bioelectromag-netics.2002;23:168-172.
  8. Huuskonen H., Juutilainen J., Komulainen H. Effects of low frequency magnetic fields on fetal development in rats.Bioelectromagnetics.1993;14: 205-213.
  9. Chiang H., Wu R.Y., Shao B.J., Fu Y.D., Yao G.D., Lu D.J.Pulsed magnetic field from video display terminals enhances teratogenic effects of cytosine arabinoside in mice. Bioelectromagne-tics.1995;16:70-74.
  10. Soleimani Rad J., Rowshangar L., Karimi K. The effect of electromagnetic field on endometri-um. 17th Annual Meeting of ESHRE. Hum Rep-rod.2001;16(Abs1):p-190.
  11. سليماني راد جعفر، روشنگر ليلا، كريمي خسرو. اثرهاي ميدان الكترومغناطيس بر روند فوليكولوژنز در تخمدان. مجله علوم تشريح ايران، سال اول، زمستان81، شماره 1، صفحات 47-51.
  12. Gougeon A. Regulation of ovarian follicular development in primates: facts and hypotheses. Endo Rev.1996;17:121-155.
  13. Williams G.T., Smith C.A. Molecular regula-tion of apoptosis: genetic controls on cell death. Cell.1993;74:777-779.
  14. Wyllie A.H. Glucocorticoid-induced thymoc-yte apoptosis is associated with endogenous endo-nuclease activation. Nature.1980;284:555-556.
  15. Billg H., Furuta I., Hsueh A.J.Estrogens inhi-bit and androgens enhance ovarian granulose cell apoptosis. Endocrinology.1993;133:2204-2212.
  16. Chun S.Y., Eisenhauer K.M., Minami S., Bill-ig H., Perlas E., Hsueh A.J .Hormonal regulation of apoptosis in early antral follicles. Endocrino-logy.1996;137:1447-1456.
  17. Tilly J.L., Kawalski K.I., Schomberg D.W., Hsueh A.J. Apoptosis in atretic ovarian follicles is associated with selective decreases in messenger ribbonucleic acid transcripts for gonadotropin receptors and cytochrome P450 aromatase. Endo-crinology.1992;131:1670-1676.
  18. Bair C.H., Chung C.S., Vasilevskaya I.A., Chang W. Isolation and characterization of a Chinese hamster ovary mutant cell line with alt-ered sensitivity to vaccinia virus killing. J Virol. 1996;70:4655-4666.
  19. Tilly J.L., Kowalski K.I., Johnson A.L., Hsu-eh A.J.W. Involvement of apoptosis in ovarian follicular atresia and postovulatory regression. Endocrinology.1991;129:2799-2801.
  20. Kasuya K. The process of apoptosis during the follicular epithelial cells in the rabbit ovary with special reference to involvement by macrop-hages. Arch Histol.1995;58:257-264.
  21. Jolly P.D.,Tisdall T.J., Heath D.A., Kun S., Mcnatty K.P. Apoptosis in bovine granulose cells in relation to steroid synthesis, cyclic adenosine 3’,5’- monophosphate response to follicle stimula-ting hormone and luteinizing hormone, and foll-icular atresia. Biol Reprod.1994;51:934-944.
  22. Devine P.J., Payne C.M., Mccuskey M. K. Ultrastructural evaluation of oocytes during atres-ia in rat ovarian follicles. Biol Reprod.2000;63: 1245-1252.
  23. Hurwitz A., Adashi E.Y. Ovarian follicular atresia as an apoptotic process. Mol Cell Endo-crinol.1991;84:19-23.
  24. Byskov A.G.S. Cell kinetic studies of follicu-lar atresia in the mouse ovary. J Reprod Fertil. 1974;37:277-285.
  25. Gaytan F., Morales C., Bellido C., Aguilar E. Ovarian follicle macrophages:Is follicular atresia in the immature rat a macrophage mediated event? Biol Reprod.1998;58:52-59.
  26. Leonard E.J., Skeel A.H. Enhancement of spreading, phagocytosis and chemotaxis by macr-ophage stimulating protein (MSP). Adv Exp Med Biol.1980;121B:181-194.
  27. Soleimani Rad J., Roushangar L. Inhibitory effect of electromagnetic field on folliculogenesis: with and without HMG- induced ovulation. MEFS 2000, Beirut; November 15-18.
  28. Cecconi S., Gualtieri G., DiBartolomeo A., Troiani G., Cifone M.G., Canipari R. Evaluation of the effects of extremely low frequency electro-magnetic fields on mammalian follicle develop-ment. Hum Reprod.2000;15(11):2319-25.
  29. Yamaguchi D.T., Huang J., Wang P.K. Inhibi-tion of Gap Junction infercellular communication by extremely low- frequency electromagnetic fiel-ds in osteoblast- like Models is dependent on cell differentiation. J Cellular Physiol.2002;190:180-8.
  30. Fomenico D., Silvestri S.Biological effects of exposure to magnetic resonance imaging: an over-view. Biomed Engin.2004;3:1-12.
  31. Bredt D.S., Snyder S.H .Nitric oxide: a phy-siologic messenger molecule. Ann Rev Biochem. 1994;63:175-195.
  32. Sugino N., Shimamura K., Tamura H., Ono M., Nakamura Y., Ogino K. Progesterone inhibits superoxide radical production by mononuclear phagocytes in pseudopregnant rats.Endocrinology. 1996;137:749-754.
  33. Foghi A., Teeds K.j., Van der Donk H., Dorr-ington J. Induction of apoptosis in rat thecal/ interstitial cells by transforming growth factor plus transforming growth factor ? in vitro. J Endocrinol.1997;153:169-17.
  34. Elfont EA., Roszka J.P., Dimino M .J. Cyto-chemical studies of acids phosphatase in ovarian follicles: a suggested role for lysosomes in steroi-dogenesis. Biol Reprod.1977;17:787-795.


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