Sedigheh Ayaty Corresponding Author
- Department of Obstet . and Gynecol .Faculty of Medicine, Mashhad Medical Sciences University, Mashad, Iran
Zohreh Yousefi
- Department of Obstet . and Gynecol .Faculty of Medicine, Mashhad Medical Sciences University, Mashad, Iran
Mohammad Taghi Shakeri
- Department of Community Medicine & Public Health, Faculty of Medicine, Mashad University of Medical Sciences, Mashad, Iran

Received: 4/1/2006 Accepted: 4/1/2006 - Publisher : Avicenna Research Institute

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Introduction: Because of improvements in the measurement methods of FSH levels in serum, there is less interest in evaluating vaginal pH for the diagnosis of menopause nowadays. The traditional test to diagnose menopause has been FSH, so far. In recent studies it has been shown that diagnosis of menopause by testing for vaginal pH is a simple, non-invasive and inexpensive method .This study intended to compare diagnostic value of vaginal pH and serum FSH in the diagnosis of menopause. Materials & Methods: This article is a descriptive cross-sectional study taking place from June to July 2005. 120 menopausal women, aged 31 to 95 years that were being referred to a meno-pausal clinic in Qaem Hospital during the study, were chosen by a target-based nonrandom sampling technique for the study. A questionnaire was used for collecting data from the patients. In addition, pH test strips were used for vaginal pH measurements and the patients' serum FSH were evaluated in a definite laboratory and their clinical signs and symptoms were recorded. The data was analyzed by SPSS software and the statistical significant of the results were tested by 2 in comparison with the kappa coefficient of agreement. P0.05 was consi-dered as the significance level of the study. Results: According to the gathered data in the study, the average vaginal pH in the patients, in the absence of vaginal infections, was 5.330.53 (with a minimum of 4.1 and a maximum of 5) With attention to the cut of point of 4.5 for vaginal pH and the baseline FSH >20 mIU/ml that characterizes menopause, the sensitivity of pH-meter in diagnosing menopause was determined to be 97%. The mean average and the standard deviation of FSH levels in this study was 80.79 6.42. Conclusion: Vaginal pH sensitivity in the diagnosis of menopause is similar to serum FSH and could be used as a suitable alternative.

Keywords: Menopause, Follicle stimulating hormone, Vaginal pH, Diagnosis, Sensitivity

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  1. Cedars M.I., Euans M., Scott J.R., Gibbs R.S., KarLan B.Y., Haney A.F. Danforth’s obstetrics and gynecolo-gy. 9th. Philadelphia: Lippincott Williams and Wilkins. 2003;pp:721-739.
  2. Speroff L., Frits M.A. Clinical Gynecologic Endocrino-logy and in fertility .7th Edition. Philadelphia:lippincott Williams & wilkins publishers.2005;pp:621-640.
  3. Mohammad K., Sadat Hashemi S.M., Farahani F.K. Age at natural menopause in Iran. Maturitas.2004;49 (4):321-6.
  4. semmens J.P., Wagner G. Estrogen deprivation and vaginal function in postmenopausal women. JAMA 1982;248:445-8.
  5. Ouslander J.G., Cooper E.B.S., Godley D. Estrogen treatment for incontinence in frail older women. J Am Geriatr Soc.1999;47:1383-4.
  6. Cruickshank R., Sharman A. The biology of the vagina in human subjects: I glycogen in the vaginal epithelium and its relation to ovarian activity , II , the bacterial sura and secretion of the vagina at various age periods and their relation to glycogen in the vaginal epithelium, III, vaginal discharge of non-infective origin. J Obstet Gynecol Br Empire.1934;41:190-226.
  7. Roz R., staumm W.E. A controlled trial of intr avaginal estriol in postmenopausal women with recurrent urinary tract infections. N Engl Med.1993;329:753-6.
  8. Rein M.F., Multer M. Trichomonas vaginalis and trichomoniasis. In: Holmes KK. Mard P-A, Sparling PF, Wiesner PJ, Cates Jr, W, Lemon SM, et al. Sexual-ly transmitted diseases. 2nd ed. New York: McGraw- Hill;1990.pp:481-92.
  9. Boskey E.R., Telsch K.M., Whaley K.J., Moench T.R., Cone R.A. Acid production by vaginal flora in vitro is consistent with the rate and extent of vaginal acidifica-tion. Infect Immun.1999;67(10):5170-5.
  10. Lang W. Vaginal acidity and PH: a review. Obstet Gynecol Surv.1955;10:546-60.
  11. Rakoff A.E., Feo L.G., Goldstein L. The biological characteristics of the normal vagina. Am J Obstet Gynecol.1944;47:467-94.
  12. Elia G., Bergman A. Estrogen effects on the urethra: beneficial effects in women with genuine stress incon-tinence. Obstet Gynecol Surv.1993;48(7):509-17.
  13. Caillouette J.C., Sharp C.F., Zimmerman G.J., Ros S. Vaginal PH as a marker for bacterial pathogens and menopausal status. Am J Obstet Gynecol.1997;176(6): 1275-7.
  14. Milsom I., Arvidsson L., Ekelund P., Molander U., Eriksson O. Factors influencing vaginal cytology, PH and bacterial flora in elderly women. Acta Obstet Gynecol Scand.1993;72(4):286-91.
  15. Pandit L., Ouslander J.G. Postmenopausal vaginal atrophy and atrophic vaginitis. Am J Med Sci.1997; 314(4):228-31.
  16. Roy S., Caillouette J.C., Roy T., Faden S. Vaginal PH is similar to follicle-stimulating hormone for meno-pause diagnosis. Am J Obstet Gynecol.2004;190(5): 1272-7.
  17. Melis G.B., Ibba M.T., Steri B., Kotsonis P., Matta V., Paoletti A.M. Role of pH as a regulator of Vaginal physiological environment. Minerva Ginecol.2000;52 (4):111-21.
  18. Garcia-Closas M., Herrero R., Bratti C., Hilde sheim A., Sherman M.E., Morera L.A., Sehiffman M. Epide-miologic determinats of vaginal PH. Am J Obstet Gynecol.1999;180(5):1060-6.
  19. Samsioe G. Urogenital aging: a hidden Problem. Am J Obstet Gynecol.1998;178(5):S 245-9.
  20. Notelovitz M. Estrogen therapy in the management of problems associated with urogenital ageing; a simple diagnostic test and the effect of the route of hormone administration. Maturitas.1995;22(suppls):31-3.
  21. Roy S., Caillouette J.C., Faden J.S., Roy T., Ramos D.E., Improving appropriate use of antifungal medica-tions: the role of an over-the counter vaginal PH self- test device. Infect Dis Obstet Gynecol.2003;11(4):209-16.
  22. Casper F., Petri E., Vaginal ring study group. Local treatment of urogenital atrophy with an estradiol-releasing vaginal ring a comparative and a placebo-controlled multicenter study. Vaginal Ring study Group. Int Urogynecol. J Pelvic Floor Dyshunct.1999; 10(3):171-6.
  23. Nilsson K., Risberg B., Heimer G. The vaginal epithelium in the postmenopause- cytology, histology and PH as methods of assessment. Maturitas.1995;21 (1):51-6.


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