Arefeh Jafarian
- Department of Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Mohammad Mehdi Akhondi Corresponding Author
- Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Nooshabeh Pezhhan
- Department of Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Mohammad Reza Sadeghi
- Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Amir Hassan Zarnani
1- Nanobiotechnology Research Center, Avicenna Research Institute (ACECR), Tehran, Iran
2- Department of Immunology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
Sheida Salehkhou
- Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

Received: 11/26/2008 Accepted: 1/17/2009 - Publisher : Avicenna Research Institute

Related Articles


Other Format



Introduction: Loss of spermatogenesis following chemo or radiotherapy for the treatment of malignancies leads to the patients temporary or permanent infertility. Restoration of sperma-togenesis after malignancy treatments is the main target of recent studies. Therefore, this study was undertaken to evaluate role of follicular stimulating hormone (FSH) and estradiol in the regeneration of spermatogenesis in azoospermic mice.Material and Methods: Busulfan, 30mg/kg, was used to induce azoospermia, in 20 male C57Bl/6 mice. Later on, the mice were divided into four groups of five animals. The animals on groups one to three received daily injections of FSH (7.5IU), estradiol benzoate (EB) (12.5μg) and simultaneous FSH and EB, respectively for ten days with no medication for the control group. On the 11th day, serum testosterone levels were measured. After sacrificing the animals, one testis of each mouse was fixed and processed for histopathological studies and the other was used for DNA flow cytometry to count haploid cells.Results: The highest increase in testosterone levels was seen with concomitant use of FSH and estradiol. The highest increases in haploid cells were seen in solitary use of estradiol and its concomitant use with FSH and resumption of spermatogenesis were observed histologically in these two kinds of administrations (p<0.001 ).conclusion: fsh unlike estradiol did not restore spermatogenesis in azoospermic mice. simultaneous use of fsh and estradiol had synergistic effects in the restoration of spermatogenesis in azoospermic mice. therefore, the concomitant use of the two hormones may be considered for the restoration of spermatogenesis in men who have undergone treatments for malignancies.< pan>

Keywords: Azoospermia, Busulfan, Chemotherapy, Estradiol, FSH, Haploid cell, Infertility, Spermiogenesis, Testosterone

To cite this article:

Figures, Charts, Tables


  1. Walker WH, Cheng J. FSH and testosterone signaling in Sertoli cells. Reproduction. 2005; 130(1): 15-28. Re-view.   [PubMed]
  2. Fan QR, Hendrickson WA. Structure of human follicle-stimulating hormone in complex with its receptor. Nature. 2005; 433(7023): 269-77.   [PubMed]
  3. Hess RA. Estrogen in the adult male reproductive tract: a review. Reprod Biol Endocrinol. 2003; 1: 52.Review.   [PubMed]
  4. Akingbemi BT. Estrogen regulation of testicular func-tion. Reprod Biol Endocrinol. 2005; 3: 51. Review.   [PubMed]
  5. Anjamrooz SH, Movahedin M, Mowla SJ, Bairanvand SP. Assessment of morphological and functional changes in the mouse testis and epididymal sperms following busulfan treatment. Iran Biomed J. 2007; 11(1): 15-22.   [PubMed]
  6. Hemsworth BN, Jackson H. Effect of Busulphan on the developing gonad of the male rat. J Reprod Fertil. 1963; 5: 187-94.   [PubMed]
  7. Sierens JE, Sneddon SF, Collins F, Millar MR, Saunders PT. Estrogens in testis biology. Ann N Y Acad Sci. 2005; 1061: 65-76. Review.   [PubMed]
  8. Meistrich ML, Shetty G. Inhibition of spermatogonial differentiation by testosterone. J Androl. 2003; 24(2): 135-48. Review.   [PubMed]
  9. Kula K, Walczak-Jedrzejowska R, S?owikowska-Hilczer J, Oszukowska E. Estradiol enhances the stimulatory effect of FSH on testicular maturation and contributes to precocious initiation of spermatogenesis. Mol Cell Endocrinol. 2001; 178(1-2): 89-97.   [PubMed]
  10. Meistrich ML. Hormonal stimulation of the recovery of spermatogenesis following chemo- or radiotherapy. APMIS. 1998 ; 106(1): 37-45. Review.   [PubMed]
  11. Delbès G, Levacher C, Habert R. Estrogen effects on fetal and neonatal testicular development. Reproduc-tion. 2006; 132(4): 527-38. Review.   [PubMed]
  12. Eddy EM, Washburn TF, Bunch DO, Goulding EH, Gladen BC, Lubahn DB, et al. Targeted disruption of the estrogen receptor gene in male mice causes alter-ation of spermatogenesis and infertility. Endocrinology. 1996 ; 137(11): 4796-805.   [PubMed]
  13. Toyama Y, Hosoi I, Ichikawa S, Maruoka M, Yashiro E, Ito H, et al. beta-estradiol 3-benzoate affects sperm-atogenesis in the adult mouse. Mol Cell Endocrinol. 2001; 178(1-2): 161-8.   [PubMed]
  14. Lambard S, Carreau S. Aromatase and oestrogens in human male germ cells. Int J Androl. 2005; 28(5): 254-9. Review.   [PubMed]
  15. MacCalman CD, Getsios S, Farookhi R, Blaschuk OW. Estrogens potentiate the stimulatory effects of follicle-stimulating hormone on N-cadherin messenger ribonucleic acid levels in cultured mouse Sertoli cells. Endocrinology. 1997; 138(1): 41-8.   [PubMed]
  16. Dorrington JH, Bendell JJ, Khan SA. Interactions between FSH, estradiol-17 beta and transforming growth factor-beta regulate growth and differentiation in the rat gonad. J Steroid Biochem Mol Biol. 1993; 44(4-6): 441-7. Review.   [PubMed]
  17. Fujita K, Ohta H, Tsujimura A, Takao T, Miyagawa Y, Takada S, et al. Transplantation of spermatogonial stem cells isolated from leukemic mice restores fertility without inducing leukemia. J Clin Invest. 2005; 115(7): 1855-61.   [PubMed]
  18. Ogawa T. Spermatogonial transplantation: the prin-ciple and possible applications. J Mol Med. 2001; 79(7): 368-74. Review.   [PubMed]


Home | About Us | Current Issue | Past Issues | Submit a Manuscript | Instructions for Authors | Subscribe | Search | Contact Us

"Journal of Reproduction & Infertility" is owned, published, and managed by Avicenna Research Institute .
Creative Commons License

This work is licensed under a Creative Commons Attribution –NonCommercial 4.0 International License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.

Journal of Reproductoin and Infertility (JRI) is a member of COMMITTEE ON PUBLICATION ETHICS . Verify here .

©2024 - eISSN : 2251-676X, ISSN : 2228-5482, For any comments and questions please contact us.