Background: Endosulfan is an organochlorine compound with insecticidal and acaricidal properties and also with widespread agricultural use for insect control. The poison could enter the body through respiration, absorption via skin and ingestion in human beings, or grazing by farm animals. The long-term effects of endosulfan EC 35% on sex hormones and sperm morphology were studied in mature male rats. Methods: In this study, 40 male rats were divided into five groups: the control group did not receive any substances while the placebo group received normal saline and the three test groups, respectively received endosulfan 5, 10 and 20 ml/kg of the total body weight every two days for three weeks. At the end of the experiment, the rats were anesthetized by chloroform and blood samples were collected from their heart for sex hormone evaluation. The rats were later sacrificed and their testes and epididymides were harvested for morphological studies of sperm. Results: Following endosulfan administration, LH and FSH concentrations increased significantly (p<0.05 ) while testosterone underwent a meaningful decrease. moreover, reproductive parameters such as sperm count, motility and testicular weight decreased significantly compared to the control group (p<0.05). conclusion: it seems that endosulfan has an undeniably damaging effect on the testis accompanied by its unfavorable effects on the reproductive system which may lead to infertility due to the changes in sex hormones concentration and sperm count and motility.< pan>0.05>
Keywords: Endosulfan, FSH, LH, Rat, Sperm count, Testosterone, Male infertility, Sex hormone, Sperm motility To cite this article:
- Oliveira-Silva JJ, Alves SR, Meyer A, Perez F, Sarcinelli PN, da Costa Mattos RC, et al. [Influence of socioeconomic factors on the pesticides poisoning, Brazil]. Rev Saude Publica. 2001;35(2):130-5. Portuguese.
- Hela DG, Lambropoulou DA, Konstantinou IK, Albanis TA. Environmental monitoring and ecological risk assessment for pesticide contamination and effects in Lake Pamvotis, northwestern Greece. Environ Toxicol Chem. 2005;24(6):1548-56.
- Betancourt M, Reséndiz A, Fierro EC. Effect of two insecticides and two herbicides on the porcine sperm motility patterns using computer-assisted semen analysis (CASA) in vitro. Reprod Toxicol. 2006;22 (3):508-12.
- Soto AM, Sonnenschein C, Chung KL, Fernandez MF, Olea N, Serrano FO. The E-SCREEN assay as a tool to identify estrogens: an update on estrogenic environmental pollutants. Environ Health Perspect. 1995;103 Suppl 7:113-22.
- Jamil K, Shaik AP, Mahboob M, Krishna D. Effect of organophosphorus and organochlorine pesticides (monochrotophos, chlorpyriphos, dimethoate, and endosulfan) on human lymphocytes in-vitro. Drug Chem Toxicol. 2004;27(2):133-44.
- Sinha N, Adhikari N, K Saxena D. Effect of endosulfan during fetal gonadal differentiation on spermatogenesis in rats. Environ Toxicol Pharmacol. 2001; 10(1-2):29-32.
- Grünfeld HT, Bonefeld-Jorgensen EC. Effect of in vitro estrogenic pesticides on human oestrogen receptor alpha and beta mRNA levels. Toxicol Lett. 2004;151(3):467-80.
- Walsh LP, Webster DR, Stocco DM. Dimethoate inhibits steroidogenesis by disrupting transcription of the steroidogenic acute regulatory (StAR) gene. J Endocrinol. 2000;167(2):253-63.
- Vittozzi L, Fabrizi L, Di Consiglio E, Testai E. Mechanistic aspects of organophosphorothionate toxicity in fish and humans. Environ Int. 2001;26 (3):125-9.
- Gaido K, Dohme L, Wang F, Chen I, Blankvoort B, Ramamoorthy K, et al. Comparative estrogenic activity of wine extracts and organochlorine pesticide residues in food. Environ Health Perspect. 1998;106 Suppl 6:1347-51.
- Boereboom FT, van Dijk A, van Zoonen P, Meulenbelt J. Nonaccidental endosulfan intoxication: a case report with toxicokinetic calculations and tissue concentrations. J Toxicol Clin Toxicol. 1998; 36(4):345-52.